Adult heart muscle cells: Isolation, properties and applications

Front Matter....Pages 3-8
Isolated adult heart cells. The development of a new experimental model....Pages 9-12
Ca-tolerant guinea-pig ventricular myocytes as isolated by pronase in the presence of 250 µM free calcium....Pages 13-17
Guinea-pig atrial cardioballs....Pages 19-22
Determination of isolated myocyte viability: Staining methods and functional criteria....Pages 23-29
Sarcolemma-bound calcium. Its importance for cell viability....Pages 31-35
Anoxic injury of adult cardiac myocytes....Pages 37-41
Non-invasive measurements of cell surface receptors....Pages 43-46
Surface antigens of adult heart cells and their use in diagnosis....Pages 47-51
Substrate utilization of adult cardiac myocytes....Pages 53-56
Characteristics of lactate transfer in isolated cardiac myocytes....Pages 57-60
The insulin receptor of adult heart muscle cells....Pages 61-64
Kinetics of cellular uptake of tracers used in myocardial scintigraphy....Pages 65-68
Adhesion of cardiac myocytes to extracellular matrix components....Pages 69-74
Development of new intercellular contacts between adult cardiac myocytes in culture....Pages 75-78
Long-term primary cultures of adult human and rat cardiomyocytes....Pages 79-82
Morphological dedifferentiation of adult cardiac myocytes in coculture with hepatocytes....Pages 83-86
Electrophysiological properties of isolated ventricular myocytes....Pages 87-91
The effect of cyanide on the K-current in guinea-pig ventricular myocytes....Pages 93-96
Measurements of single-channel currents in the membrane of isolated cells: ATP-dependence of K + -channels....Pages 97-99
Evidence for Ca-mediated inactivation of I Ca in dialysed guinea-pig atrial cardioballs....Pages 101-105
Cardiac membrane currents and energetic state....Pages 107-110
Simultaneous measurements of action potentials and contractions in single cultured adult and embryonic heart muscle cells....Pages 111-115
The two components in the shortening of unloaded ventricular myocytes: Their voltage dependence....Pages 117-121
Temperature dependence of verapamil action....Pages 123-127
Microtubules and desmin filaments during the onset of heart growth in the rat....Pages 129-132
The correlation between catecholamine and lipid peroxidation induced damage in heart cells....Pages 133-136
Anoxia in neonatal rat heart cell cultures....Pages 137-142
Enzyme release and glycolytic energy production....Pages 143-147
Anoxia influences the lateral diffusion of a lipid probe in the plasma membrane of isolated cardiac myocytes....Pages 149-152
Summaries of the discussions of the symposium....Pages 153-159
Abstracts of the poster-session on the adult myocyte model....Pages 161-164
Back Matter....Pages 165-166