Adenylyl Cyclase, G Proteins, and Guanylyl Cyclase

Content: 
Contributors to volume 195
Pages ix-xii

Preface
Pages xiii-xiv
Roger A. Johnson, Jackie D. Corbin

Volumes in series
Pages xv-xxx

[1] Assay of adenylyl cyclase catalytic activity Original Research Article
Pages 3-21
Roger A. Johnson, Yoram Salomon

[2] Cellular responsiveness to hormones and neutrotransmitters: Conversion of [3H]Adenine to [3H]cAMP in cell monolayers, cell suspensions, and tissue slices Original Research Article
Pages 22-28
Yoram Salomon

[3] Preparation of О±-32P-Labeled nucleoside triphosphates, nicotinamide adenine dinucleotide, and cyclic nucleotides for use in determining adenylyl and guanylyl cyclases and cyclic nucleotide phosphodiesterase Original Research Article
Pages 29-44
Timothy F. Walseth, Peter S.T. Yuen, Malcolm C. Moos Jr.

[4] Synthesis of forskolin-agarose affinity matrices Original Research Article
Pages 44-51
Thomas Pfeuffer

[5] High-affinity binding sites for [3H]forskolin Original Research Article
Pages 52-65
Antonio Laurenza, Kenneth B. Seamon

[6] Purification and characterization of calmodulin-sensitive adenylyl cyclase from bovine brain Original Research Article
Pages 65-82
Ping Wang, Daniel R. Storm

[7] Purification of adenylyl cyclase from heart and brain Original Research Article
Pages 83-91
Elke Pfeuffer, Stefan Mollner, Thomas Pfeuffer

[8] Calmodulin-mediated adenylyl cyclase from equine sperm Original Research Article
Pages 91-110
William A. Toscano Jr., Mary K. Gross

[9] Isolation of polyclonal antibodies against bovine brain calmodulin-sensitive adenylyl cyclases Original Research Article
Pages 110-121
Gary B. Rosenberg, Ping Wang, Daniel R. Storm

[10] Characteristics and use of monoclonal antibodies to various forms of adenylyl cyclase Original Research Article
Pages 121-129
Stefan Mollner, Thomas Pfeuffer

[11] Purification of soluble form of adenylyl cyclase from testes Original Research Article
Pages 130-136
Theodor Braun

[12] Purification and assay of cell-invasive form of calmodulin-sensitive adenylyl cyclase from Bordetella pertussis Original Research Article
Pages 137-152
H.Robert Masure, Maura G. Donovan, Daniel R. Storm

[13] Purification and characterization of adenylyl cyclase from Bacillus anthracis Original Research Article
Pages 153-168
Stephen H. Leppla

Preparation and application of GTP-agarose matrices Original Research Article
Pages 171-177
Elke Pfeuffer, Thomas Pfeuffer

[15] Purification of G proteins Original Research Article
Pages 177-188
Juan Codina, Donna J. Carty, Lutz Birnbaumer, Ravi Iyengar

[16] Preparation of guanine nucleotide-free G proteins Original Research Article
Pages 188-192
Kenneth M. Ferguson, Tsutomu Higashijima

[17] Purification of recombinant GsО± Original Research Article
Pages 192-202
Michael P. Graziano, Michael Freissmuth, Alfred G. Gilman

[18] Purification of recombinant G1О± and GoО± proteins from Escherichia coli Original Research Article
Pages 202-215
Maurine E. Linder, Alfred G. Gilman

[19] Synthetic peptide antisera with determined specificity for G protein О± or ОІ subunits Original Research Article
Pages 215-233
Susanne M. Mumby, Alfred G. Gilman

[20] Quantitation and purification of ADP-ribosylation factor Original Research Article
Pages 233-242
Richard A. Kahn

[21] Soluble guanine nucleotide-dependent ADP-ribosylation factors in activation of adenylyl cyclase by cholera toxin Original Research Article
Pages 243-256
Joel Moss, Su-Chen Tsai, S. Russ Price, David A. Bobak, Martha Vaughan

[22] ADP-ribosylation of G proteins with pertussis toxin Original Research Article
Pages 257-266
Gregory S. Kopf, Marilyn J. Woolkalis

[23] Cholera toxin-catalyzed [32P]ADP-ribosylation of proteins Original Research Article
Pages 267-280
D. Michael Gill, Marilyn J. Woolkalis

[24] Photoaffinity labeling of GTP-binding proteins Original Research Article
Pages 280-286
Rolf Thomas, Thomas Pfeuffer

[25] Identification of receptor-activated G proteins with photoreactive GTP analog, [О±-32P]GTP azidoanilide Original Research Article
Pages 286-301
Stefan Offermanns, GГјnter Schultz, Walter Rosenthal

[26] Quantitative immunoblotting of G-protein subunits Original Research Article
Pages 302-315
Donna J. Carty, Richard T. Premont, Ravi Iyengar

[27] Assay of g-protein ОІОі-subunit complex by catalytic support of ADP-ribosylation of GoО± Original Research Article
Pages 315-321
Patrick J. Casey, Iok-Hou Pang, Alfred G. Gilman

[28] Tryptophan fluorescence of G proteins: Analysis of guanine nucleotide binding and hydrolysis Original Research Article
Pages 321-328
Tsutomu Higashijima, Kenneth M. Ferguson

[29] Reconstitution of receptors and G proteins in phospholipid vesicles Original Research Article
Pages 329-342
Richard A. Cerione, Elliott M. Ross

[30] Assay of guanylyl cyclase catalytic activity Original Research Article
Pages 345-355
Steven E. Domino, D. Janette Tubb, David L. Garbers

[31] Preparative polyacrylamide gel electrophoresis apparatus for purification of guanylyl cyclase Original Research Article
Pages 355-363
Alexander MГјlsch, Rupert Gerzer

[32] Detergent interactions and solubilization techniques for membrane guanylyl cyclase Original Research Article
Pages 363-373
Arnold A. White, Pushkaraj J. Lad

[33] Purification of membrane form of guanylyl cyclase Original Research Article
Pages 373-377
Chodavarapu S. Ramarao, David L. Garbers

[34] Purification of heme-containing soluble guanylyl cyclase Original Research Article
Pages 377-383
Alexander MГјlsch, Rupert Gerzer

[35] Preparation of soluble guanylyl cyclase from bovine lung by immunoaffinity chromatography Original Research Article
Pages 384-391
Peter Humbert, Feraydoon Niroomand, Gabriela Fischer, Bernd Mayer, Doris Koesling, Klaus-Dieter Hinsch, Günter Schultz, Eycke Böhme

[36] Immunoaffinity purification of soluble guanylyl cyclase Original Research Article
Pages 391-396
Scott A. Waldman, Dale C. Leitman, Ferid Murad

[37] Copurification of atrial natriuretic peptide receptor and particulate guanylyl cyclase Original Research Article
Pages 397-404
Scott A. Waldman, Dale C. Leitman, Ferid Murad

[38] Copurification of atrial natriuretic factor receptors and guanylyl cyclase from adrenal cortex Original Research Article
Pages 404-413
Tadashi Inagami, Ryoichi Takayanagi, Rudolf M. Snajdar

[39] Molecular cloning of membrane forms of guanylyl cyclase Original Research Article
Pages 414-423
Sujay Singh, David L. Garbers

[40] Radiation-inactivation analysis of multidomain proteins: The case of particulate guanylyl cyclase Original Research Article
Pages 423-435
Michel Potier, CГ©line Huot, Caroline Koch, Pavel Hamet, Johanne Tremblay

[41] Identification of atrial natriuretic peptide receptors in cultured cells Original Research Article
Pages 436-446
Dale C. Leitman, Scott A. Waldman, Ferid Murad

[42] Evaluating atrial natriuretic peptide-induced cGMP production by particulate guanylyl cyclase stimulation in Vitro and in Vivo Original Research Article
Pages 447-461
Pavel Hamet, Johanne Tremblay

[43] Phosphorylation and dephosphorylation of sea urchin sperm cell guanylyl cyclase Original Research Article
Pages 461-466
J. Kelley Bentley

[44] Calcium-regulated guanylyl cyclases from Paramecium and Tetrahymena Original Research Article
Pages 466-474
Joachim E. Schultz, Susanne Klumpp

Author index
Pages 475-494

Subject index
Pages 495-512