Adeno-Associated Virus Vectors: Design and Delivery

Front Matter ....Pages i-xii
Front Matter ....Pages 1-1
 Design of AAV Vectors for Delivery of RNAi  (Florie Borel, Christian Mueller)....Pages 3-18
 Design of AAV Vectors for Delivery of Large or Multiple Transgenes  (Aman Patel, Junling Zhao, Dongsheng Duan, Yi Lai)....Pages 19-33
 Ligand Coupling to the AAV Capsid for Cell-Specific Gene Transfer  (Johanna Reul, Alexander Muik, Christian J. Buchholz)....Pages 35-50
 Quantitative and Digital Droplet-Based AAV Genome Titration  (Julio Sanmiguel, Guangping Gao, Luk H. Vandenberghe)....Pages 51-83
 Single-Stranded DNA Virus Sequencing (SSV-Seq) for Characterization of Residual DNA and AAV Vector Genomes  (Emilie Lecomte, Adrien Leger, Magalie Penaud-Budloo, Eduard Ayuso)....Pages 85-106
 In Situ Hybridization for Detection of AAV-Mediated Gene Expression  (Jacqueline E. Hunter, Brittney L. Gurda, Sea Young Yoon, Michael J. Castle, John H. Wolfe)....Pages 107-122
 Use of AAV Vectors for CRISPR-Mediated In Vivo Genome Editing in the Retina  (Wenhan Yu, Zhijian Wu)....Pages 123-139
Front Matter ....Pages 141-141
 Intravenous Infusion of AAV for Widespread Gene Delivery to the Nervous System  (Dominic J. Gessler, Phillip W. L. Tai, Jia Li, Guangping Gao)....Pages 143-163
 Intraspinal and Intracortical Delivery of AAV Vectors for Intersectional Circuit Tracing in Non-transgenic Species  (James M. Conner, Greg L. Bain, Jennifer N. Dulin)....Pages 165-176
 MRI-Guided Focused Ultrasound for Targeted Delivery of rAAV to the Brain  (Zeinab Noroozian, Kristiana Xhima, Yuexi Huang, Brian K. Kaspar, Sebastian Kügler, Kullervo Hynynen et al.)....Pages 177-197
 AAV-Mediated Gene Delivery to the Spinal Cord by Intrathecal Injection  (Cristina D. Peterson, Alexander G. J. Skorput, Kelley F. Kitto, George L. Wilcox, Lucy Vulchanova, Carolyn A. Fairbanks)....Pages 199-207
 Subpial AAV Delivery for Spinal Parenchymal Gene Regulation in Adult Mammals  (Mariana Bravo-Hernández, Takahiro Tadokoro, Martin Marsala)....Pages 209-233
Front Matter ....Pages 235-235
 Peripheral AAV Injection for Retrograde Transduction of Dorsal Root and Trigeminal Ganglia  (David C. Bloom, Zachary L. Watson, Donna M. Neumann)....Pages 237-247
 SubILM Injection of AAV for Gene Delivery to the Retina  (Paul D. Gamlin, John J. Alexander, Sanford L. Boye, C. Douglas Witherspoon, Shannon E. Boye)....Pages 249-262
 Intracameral Delivery of AAV to Corneal Endothelium for Expression of Secretory Proteins  (Jeffrey O’Callaghan, Matthew Campbell, Peter Humphries)....Pages 263-270
 AAV-Mediated Gene Delivery to the Inner Ear  (Omar Akil, Lawrence Lustig)....Pages 271-282
 Intranasal Delivery of Adenoviral and AAV Vectors for Transduction of the Mammalian Peripheral Olfactory System  (Cedric R. Uytingco, Jeffrey R. Martens)....Pages 283-297
 AAV-Mediated Gene Delivery to Taste Cells of the Tongue  (Akiyuki Taruno, Makiko Kashio)....Pages 299-307
Front Matter ....Pages 309-309
 AAV Vectors for Efficient Gene Delivery to Rodent Hearts  (Estrella Lopez-Gordo, Erik Kohlbrenner, Michael G. Katz, Thomas Weber)....Pages 311-332
 AAV-Mediated Gene Delivery to the Liver: Overview of Current Technologies and Methods  (Brett Palaschak, Roland W. Herzog, David M. Markusic)....Pages 333-360
 AAV-Mediated Gene Delivery to the Lung  (Laura P. van Lieshout, Jakob M. Domm, Sarah K. Wootton)....Pages 361-372
 Simplified Purification of AAV and Delivery to the Pancreas by Intraductal Administration  (Ping Guo, John Wiersch, Xiangwei Xiao, George Gittes)....Pages 373-387
 rAAV-Mediated Gene Delivery to Adipose Tissue  (Wei Huang, Nicholas J. Queen, Lei Cao)....Pages 389-405
 AAV-Mediated Gene Delivery to the Enteric Nervous System by Intracolonic Injection  (Reshma Gore, Maureen S. Riedl, Kelley F. Kitto, Carolyn A. Fairbanks, Lucy Vulchanova)....Pages 407-415
Back Matter ....Pages 417-426