ADAMTS Proteases: Methods and Protocols

Front Matter ....Pages i-x
 ADAMTS Proteins: Concepts, Challenges, and Prospects  (Suneel S. Apte)....Pages 1-12
 Inhibition of ADAMTS1 Expression by Lentiviral CRISPR/Cas9 Gene Editing Technology  (Carlos Peris-Torres, Orlando Serrano, María del Carmen Plaza-Calonge, Juan Carlos Rodríguez-Manzaneque)....Pages 13-24
 Analyzing the Effects of O-Fucosylation on Secretion of ADAMTS Proteins Using Cell-Based Assays  (Steven John Berardinelli, Robert S. Haltiwanger)....Pages 25-43
 Challenges and Solutions for Purification of ADAMTS Proteases: An Overview  (Alain C. Colige)....Pages 45-53
 Purification of Native or Recombinant ADAMTS2, and Procollagen I Cleavage Assay  (Alain C. Colige)....Pages 55-62
 ADAMTS7: Recombinant Protein Expression and Purification  (Rens de Groot)....Pages 63-73
 Purification and Activity Determination of ADAMTS-4 and ADAMTS-5 and Their Domain Deleted Mutants  (Milan M. Fowkes, Ngee H. Lim)....Pages 75-91
 Expression and Preparation of Recombinant Reelin and ADAMTS-3 Proteins  (Takao Kohno, Himari Ogino, Yuko Yamakage, Mitsuharu Hattori)....Pages 93-104
 Assay for Reelin-Cleaving Activity of ADAMTS and Detection of Reelin and Its Fragments in the Brain  (Himari Ogino, Yuko Yamakage, Mihoshi B. Yamashita, Takao Kohno, Mitsuharu Hattori)....Pages 105-111
 Chemical Modification of Proteoglycanases with Biotin  (Salvatore Santamaria)....Pages 113-123
 Analysis of Aggrecanase Activity Using Neoepitope Antibodies  (Salvatore Santamaria, Kazuhiro Yamamoto)....Pages 125-136
 Molecular Cloning, Lentiviral Transduction, and Expression of Recombinant ADAMTSL2 and ADAMTSL4  (Mukti Singh, Stuart A. Cain, Clair Baldock)....Pages 137-155
 Purification of Recombinant ADAMTSL2  (Mukti Singh, Clair Baldock)....Pages 157-172
 Expression Analysis by RNAscope™ In Situ Hybridization  (Timothy J. Mead, Suneel S. Apte)....Pages 173-178
 Analysis of ADAMTS Effects on Cell Adhesion and Migration  (Jordi Lambert, Dylan R. Edwards)....Pages 179-193
 Cell-Based Interaction Analysis of ADAMTS Proteases and ADAMTS-Like Proteins with Fibrillin Microfibrils  (Dirk Hubmacher)....Pages 195-206
 Alizarin Red and Alcian Blue Preparations to Visualize the Skeleton  (Timothy J. Mead)....Pages 207-212
 Biophysical Techniques to Analyze Elastic Tissue Extracellular Matrix Proteins Interacting with ADAMTS Proteins  (Valentin Nelea, Dieter P. Reinhardt)....Pages 213-235
 Quantification of Extracellular Matrix Fiber Systems Related to ADAMTS Proteins  (Rong-Mo Zhang, Heena Kumra, Dieter P. Reinhardt)....Pages 237-250
 Visualization of Perineuronal Nets in Central Nervous System Tissue Sections  (Luke Souter, Jessica C. F. Kwok)....Pages 251-260
 Visualization and Quantification of Pericellular Matrix  (Timothy J. Mead, Suneel S. Apte)....Pages 261-264
 Quantitative Mass Spectrometry-Based Secretome Analysis as a Tool to Investigate Metalloprotease and TIMP Activity  (Chun-Yao Yang, Linda Troeberg, Simone D. Scilabra)....Pages 265-273
 Preparation and Application of a Decellularized Extracellular Matrix for Identification of ADAMTS Substrates  (Rahel Schnellmann, Ruth Chiquet-Ehrismann)....Pages 275-284
 Mapping the N-Terminome in Tissue Biopsies by PCT-TAILS  (Louise Bundgaard, Simonas Savickas, Ulrich auf dem Keller)....Pages 285-296
Back Matter ....Pages 297-299