Laser Capture Microdissection: Methods and Protocols

Front Matter ....Pages i-xv
 Laser Capture Microdissection: Insights into Methods and Applications  (Meera Mahalingam)....Pages 1-17
 Laser Microdissection-Based Microproteomics of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues  (Rémi Longuespée, Dominique Baiwir, Gabriel Mazzucchelli, Nicolas Smargiasso, Edwin De Pauw)....Pages 19-31
 Laser Microdissection Workflow for Isolating Nucleic Acids from Fixed and Frozen Tissue Samples  (Yelena G. Golubeva, Andrew C. Warner)....Pages 33-93
 Protocol for the Analysis of Laser Capture Microdissected Fresh-Frozen Tissue Homogenates by Silver-Stained 1D SDS-PAGE  (DaRue A. Prieto, Gordon Whitely, Donald J. Johann Jr., Josip Blonder)....Pages 95-110
 Next-Generation Sequencing Analysis of Laser-Microdissected Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens  (Lavinia Mägel, Stephan Bartels, Ulrich Lehmann)....Pages 111-118
 Adaptation of Laser Microdissection Technique to Nanostring RNA Analysis in the Study of a Spontaneous Metastatic Mammary Carcinoma Mouse Model  (Nadia P. Castro, Yelena G. Golubeva)....Pages 119-137
 Laser Capture Microdissection as a Tool to Study the Mucosal Immune Response in Celiac Disease  (Giuseppe Iacomino, Vera Rotondi Aufiero, Pasquale Marena, Antonella Venezia, Riccardo Troncone, Salvatore Auricchio et al.)....Pages 139-154
 Laser Capture Microdissection and Isolation of High-Quality RNA from Frozen Endometrial Tissue  (Michele Cummings, Georgia Mappa, Nicolas M. Orsi)....Pages 155-166
 Laser Microdissection for Human Papillomavirus (HPV) Genotyping Attribution and Methylation Pattern Analyses of Squamous Intraepithelial Lesions  (Monica Molano, Suzanne M. Garland, Alyssa M. Cornall)....Pages 167-189
 Laser Capture Microdissection and Transcriptional Analysis of Sub-Populations of the Osteoblast Lineage from Undecalcified Bone  (Efrain Pacheco, Rong Hu, Scott Taylor)....Pages 191-202
 Cell Type-Specific Laser Capture Microdissection for Gene Expression Profiling in the Human Brain  (Sarah A. Mauney, Tsung-Ung W. Woo, Kai C. Sonntag)....Pages 203-221
 The Isolation of Pure Populations of Neurons by Laser Capture Microdissection: Methods and Application in Neuroscience  (Renée Morris, Prachi Mehta)....Pages 223-233
 Laser Capture Microdissection in Traumatic Brain Injury Research: Obtaining Hippocampal Subregions and Pools of Injured Neurons for Genomic Analyses  (Deborah R. Boone, Harris A. Weisz, Stacy L. Sell, Helen L. Hellmich)....Pages 235-245
 Isolation of Distinct Types of Neurons from Fresh Brain Tissue Using Laser Microdissection in Combination with High-Performance Liquid Chromatography—Mass Spectrometry  (Luisa Aring, Simone Steinbach, Katrin Marcus, Caroline May)....Pages 247-260
 Immuno-Guided Laser-Capture Microdissection of Glial Cells for mRNA Analysis  (Arnaud B. Nicot, Justine Rambeau, Flora Guillot, Alexandra Garcia, David A. Laplaud)....Pages 261-271
 Immuno-Laser-Capture Microdissection for the Isolation of Enriched Glial Populations from Frozen Post-Mortem Human Brain  (Julie E. Simpson, Stephen B. Wharton, Paul R. Heath)....Pages 273-284
 Laser-Capture Microdissection for the Analysis of Rat and Human Spinal Cord Ependyma by qPCR  (Daniel Garcia-Ovejero, Beatriz Paniagua-Torija, Angel Arevalo-Martin, Beatriz Navarro-Galve, Eduardo Molina-Holgado)....Pages 285-318
 Isolation of Amyloid Plaques and Neurofibrillary Tangles from Archived Alzheimer’s Disease Tissue Using Laser-Capture Microdissection for Downstream Proteomics  (Eleanor Drummond, Shruti Nayak, Geoffrey Pires, Beatrix Ueberheide, Thomas Wisniewski)....Pages 319-334
 Cell-Specific RNA Quantification in Human SN DA Neurons from Heterogeneous Post-mortem Midbrain Samples by UV-Laser Microdissection and RT-qPCR  (Johanna Duda, Michael Fauler, Jan Gründemann, Birgit Liss)....Pages 335-360
 Laser-Capture Microdissection for Layer-Specific Analysis of Enteric Ganglia  (Corinna Rosenbaum, Martina Böttner, Thilo Wedel, Marco Metzger)....Pages 361-369
 A Laser Microdissection–Liquid Chromatography–Tandem Mass Spectrometry Workflow for Post-mortem Analysis of Brain Tissue  (David C. Hondius, Jeroen J. M. Hoozemans, Annemieke J. M. Rozemuller, Ka Wan Li, August B. Smit)....Pages 371-383
 Laser-Capture Microdissection and RNA Extraction from Perfusion-Fixed Cartilage and Bone Tissue from Mice Implanted with Human iPSC-Derived MSCs in a Calvarial Defect Model  (Xiaonan Xin, Xi Jiang, Alexander Lichtler, Mark Kronenberg, David Rowe, Joel S. Pachter)....Pages 385-396
 Laser Capture Microdissection-Based RNA-Seq of Barley Grain Tissues  (Ronny Brandt, Martin Mascher, Johannes Thiel)....Pages 397-409
Back Matter ....Pages 411-412