Bacterial Multidrug Exporters: Methods and Protocols

Front Matter ....Pages i-xii
Front Matter ....Pages 1-1
 High-Resolution Crystallographic Analysis of AcrB Using Designed Ankyrin Repeat Proteins (DARPins)  (Heng Keat Tam, Viveka Nand Malviya, Klaas M. Pos)....Pages 3-24
 Crystallographic Analysis of Drug and Inhibitor-Binding Structure of RND-Type Multidrug Exporter AcrB in Physiologically Relevant Asymmetric Crystals  (Ryosuke Nakashima, Keisuke Sakurai, Akihito Yamaguchi)....Pages 25-36
 Crystallographic Analysis of MATE-Type Multidrug Exporter with Its Inhibitors  (Tsukasa Kusakizako, Yoshiki Tanaka, Christopher J. Hipolito, Hiroaki Suga, Osamu Nureki)....Pages 37-57
 Crystallographic Analysis of the CusBA Heavy-Metal Efflux Complex of Escherichia coli  (Jared A. Delmar, Edward W. Yu)....Pages 59-70
 Purification of AcrAB-TolC Multidrug Efflux Pump for Cryo-EM Analysis  (Dijun Du, Zhao Wang, Wah Chiu, Ben F. Luisi)....Pages 71-81
 NMR Spectroscopy Approach to Study the Structure, Orientation, and Mechanism of the Multidrug Exporter EmrE  (Maureen Leninger, Nathaniel J. Traaseth)....Pages 83-96
 Generation of Conformation-Specific Antibody Fragments for Crystallization of the Multidrug Resistance Transporter MdfA  (Frank Jaenecke, Yoshiko Nakada-Nakura, Kumar Nagarathinam, Satoshi Ogasawara, Kehong Liu, Yunhon Hotta et al.)....Pages 97-109
Front Matter ....Pages 111-111
 Biochemical Reconstitution and Characterization of Multicomponent Drug Efflux Transporters  (Martin Picard, Elena B. Tikhonova, Isabelle Broutin, Shuo Lu, Alice Verchère, Helen I. Zgurskaya)....Pages 113-145
 Covalently Linked Trimers of RND (Resistance-Nodulation-Division) Efflux Transporters to Study Their Mechanism of Action: Escherichia coli AcrB Multidrug Exporter as an Example  (Hiroshi Nikaido)....Pages 147-165
 Determining Ligand Path Through a Major Drug Transporter, AcrB, in Escherichia coli  (Fasahath Husain, Hiroshi Nikaido)....Pages 167-175
Front Matter ....Pages 177-177
 Molecular Modeling of Multidrug Properties of Resistance Nodulation Division (RND) Transporters  (Pierpaolo Cacciotto, Venkata K. Ramaswamy, Giuliano Malloci, Paolo Ruggerone, Attilio V. Vargiu)....Pages 179-219
 A Transcriptomic Approach to Identify Novel Drug Efflux Pumps in Bacteria  (Liping Li, Sasha G. Tetu, Ian T. Paulsen, Karl A. Hassan)....Pages 221-235
Front Matter ....Pages 237-237
 Regulation of the Expression of Bacterial Multidrug Exporters by Two-Component Signal Transduction Systems  (Kunihiko Nishino)....Pages 239-251
 Study of the Expression of Bacterial Multidrug Efflux Pumps in Anaerobic Conditions  (Jingjing Sun, Ziqing Deng, Danny Ka Chun Fung, Aixin Yan)....Pages 253-268
 Identification of a Staphylococcus aureus Efflux Pump Regulator Using a DNA–Protein Affinity Technique  (Que Chi Truong-Bolduc, David C. Hooper)....Pages 269-291
 High-Throughput Flow Cytometry Screening of Multidrug Efflux Systems  (Mark K. Haynes, Matthew Garcia, Ryan Peters, Anna Waller, Pietro Tedesco, Oleg Ursu et al.)....Pages 293-318
Front Matter ....Pages 319-319
 Single-Molecule Analysis of Membrane Transporter Activity by Means of a Microsystem  (Rikiya Watanabe, Naoki Soga, Shin-ya Ohdate, Hiroyuki Noji)....Pages 321-330
 Large-Scale Femtoliter Droplet Array for Single Cell Efflux Assay of Bacteria  (Ryota Iino, Shouichi Sakakihara, Yoshimi Matsumoto, Kunihiko Nishino)....Pages 331-341
 Reconstitution and Transport Analysis of Eukaryotic Transporters in the Post-Genomic Era  (Hiroshi Omote, Yoshinori Moriyama)....Pages 343-352
Back Matter ....Pages 353-355