Cancer Immunosurveillance: Methods and Protocols

Front Matter ....Pages i-xiii
 Usage of Droplet Digital PCR (ddPCR) Assays for T Cell Quantification in Cancer  (Willem H. Zoutman, Rogier J. Nell, Pieter A. van der Velden)....Pages 1-14
 An Economical, Quantitative, and Robust Protocol for High-Throughput T Cell Receptor Sequencing from Tumor or Blood  (Imran Uddin, Kroopa Joshi, Theres Oakes, James M. Heather, Charles Swanton, on behalf of the TRACERx consortium et al.)....Pages 15-42
 Generation of Tumor Antigen-Specific Cytotoxic T Lymphocytes from Pluripotent Stem Cells  (Xiaoniao Chen, Fengyang Lei, Liqiang Wang, Xiaofang Xiong, Jianxun Song)....Pages 43-55
 Expansion and Adoptive Transfer of Human Vδ2+ T Cells to Assess Antitumor Effects In Vivo  (Akshat Sharma, Nicholas A. Zumwalde, Jenny E. Gumperz)....Pages 57-72
 Usage of Single-Cell RNA Sequencing to Unveil Immune Lymphoid Cell Precursors  (Yong Yu, Pengtao Liu)....Pages 73-86
 Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance  (Monica Parodi, Raffaella Meazza, Chiara Vitale, Gabriella Pietra, Paolo Carrega, Massimo Vitale)....Pages 87-117
 CD107a Degranulation Assay to Evaluate Immune Cell Antitumor Activity  (Seila Lorenzo-Herrero, Christian Sordo-Bahamonde, Segundo Gonzalez, Alejandro López-Soto)....Pages 119-130
 A Flow Cytometric NK Cell-Mediated Cytotoxicity Assay to Evaluate Anticancer Immune Responses In Vitro  (Seila Lorenzo-Herrero, Christian Sordo-Bahamonde, Segundo González, Alejandro López-Soto)....Pages 131-139
 Mouse Models to Study Natural Killer Cell-Mediated Immunosurveillance and Metastatic Latency  (Vidhya R. Nair, Srinivas Malladi)....Pages 141-150
 Tumor-Associated Macrophage Isolation and In Vivo Analysis of Their Tumor-Promoting Activity  (Nicholas M. Clark, Paula D. Bos)....Pages 151-160
 Conditional Genetic Ablation Mouse Models as a Tool to Study Cancer Immunosurveillance In Vivo  (Magali Dupont, Christian A. J. Vosshenrich)....Pages 161-176
 Determination and Isolation of Immune Populations from Brain Tumor Microenvironments  (Javier Godino, Alberto J. Schuhmacher)....Pages 177-188
 Mouse Model of Colitis-Associated Colorectal Cancer (CAC): Isolation and Characterization of Mucosal-Associated Lymphoid Cells  (Llipsy Santiago, Marta Castro, Julián Pardo, Maykel Arias)....Pages 189-202
 Cancer Exome-Based Identification of Tumor Neo-Antigens Using Mass Spectrometry  (Shelly Kalaora, Yardena Samuels)....Pages 203-214
 Single-Cell Mass Cytometry of Archived Human Epithelial Tissue for Decoding Cancer Signaling Pathways  (Cherie’ R. Scurrah, Alan J. Simmons, Ken S. Lau)....Pages 215-229
 Purification of Leukemia-Derived Exosomes to Study Microenvironment Modulation  (Marina Wierz, Sandrine Pierson, Ernesto Gargiulo, Coralie Guerin, Etienne Moussay, Jerome Paggetti)....Pages 231-245
 Molecular Characterization of Circulating Tumor Cells to Study Cancer Immunoevasion  (Chiara Nicolazzo, Angela Gradilone, Guido Carpino, Paola Gazzaniga, Cristina Raimondi)....Pages 247-258
 Quantitative Identification of Senescent Cells in Cancer  (Anat Biran, Ziv Porat, Valery Krizhanovsky)....Pages 259-267
 Generation and Harnessing of Heterotypic Tumor-Stroma Spheroids to Study Cancer Immunosurveillance  (Dirk Zboralski, Anna Frömming)....Pages 269-281
 Establishment of Slice Cultures as a Tool to Study the Cancer Immune Microenvironment  (Xiuyun Jiang, Y. David Seo, Kevin M. Sullivan, Venu G. Pillarisetty)....Pages 283-295
 Gold Standard Assessment of Immunogenic Cell Death in Oncological Mouse Models  (Juliette Humeau, Sarah Lévesque, Guido Kroemer, Jonathan G. Pol)....Pages 297-315
 A Novel Dendritic Cell-Based Vaccination Protocol to Stimulate Immunosurveillance of Aggressive Cancers  (Annunziata Nigro, Barbara Montico, Vincenzo Casolaro, Jessica Dal Col)....Pages 317-333
 Development of Bispecific Antibody Derivatives for Cancer Immunotherapy  (Yuan He, Wijnand Helfrich, Edwin Bremer)....Pages 335-347
 Generation of CAR-T Cells for Cancer Immunotherapy  (Qumiao Xu, Hizkia Harto, Robert Berahovich, Shirley Xu, Hua Zhou, Vita Golubovskaya et al.)....Pages 349-360
Back Matter ....Pages 361-363