Xenopus laevis: Practical Uses in Cell and Molecular Biology

Content: 
Series Editor
Page ii

Editorial page
Page iii

Copyright Page
Page iv

Contributors
Pages xiii-xvi

Foreword
Pages xvii-xviii
P.D. Nieuwkoop

Preface
Pages xix-xxii
Brian K. Kay, H. Benjamin Peng

Chapter 1 Raising Xenopus in the Laboratory Original Research Article
Pages 3-18
M. Wu, J. Gerhart

Chapter 2 Genetics of Xenopus laevis Original Research Article
Pages 19-34
Jean-Daniel Graf, Hans Rudolf Kobel

Chapter 3 Experimentally Induced Homozygosity in Xenopus laevis Original Research Article
Pages 35-44
Robert Tompkins, Dana Reinschmidt

Chapter 4 Oogenesis and Oocyte Isolation Original Research Article
Pages 45-46,46a,46b,47-60
L. Dennis Smith, Weilong Xu, Robert L. Varnold

Chapter 5 Early Embryonic Development of Xenopus laevis Original Research Article
Pages 61-113
Ray Keller

Chapter 6 Vitellogenin Uptake and in Vitro Culture of Oocytes Original Research Article
Pages 117-132
Lee K. Opresko

Chapter 7 Biochemical Fractionation of Oocytes Original Research Article
Pages 133-148
Janice P. Evans, Brian K. Kay

Chapter 8 Lampbrush Chromosomes Original Research Article
Pages 149-166
Joseph G. Gall, Christine Murphy, Harold G. Callan, Zheng'an Wu

Chapter 9 Preparation of Synthetic mRNAs and Analyses of Translational Efficiency in Microinjected Xenopus Oocytes Original Research Article
Pages 167-183
Mike Wormington

Chapter 10 Use of Oligonucleotides for Antisense Experiments in Xenopus laevis Oocytes Original Research Article
Pages 185-210
Carol Prives, Diana Foukal

Chapter 11 Fertilization of Cultured Xenopus Oocytes and Use in Studies of Maternally Inherited Molecules Original Research Article
Pages 213-218,218a,218b,219-230
J. Heasman, S. Holwill, C.C. Wylie

Chapter 12 Isolation of Extracellular Matrix Structures from Xenopus laevis Oocytes, Eggs, and Embryos Original Research Article
Pages 231-247
Jerry L. Hedrick, Daniel M. Hardy

Chapter 13 Analysis of Cellular Signaling Events, the Cytoskeleton, and Spatial Organization of Macromolecules during Early Xenopus Development Original Research Article
Pages 249-270
David G. Capco, William M. Bement

Chapter 14 Generation of Body Plan Phenotypes in Early Embryogenesis Original Research Article
Pages 271-284
Ken Kao, Mike Danilchik

Chapter 15 Fluorescent Dextran Clonal Markers Original Research Article
Pages 285-294,294a,294b,295-297
Robert L. Gimlich

Capter 16 Nuclear Transplantation in Xenopus Original Research Article
Pages 299-309
J.B. Gurdon

Chapter 17 Mesoderm Induction Original Research Article
Pages 311-328
Igor B. Dawid

Chapter 18 Neural Induction Original Research Article
Pages 329-346
Carey R. Phillips

Chapter 19 Analysis of Class II Gene Regulation Original Research Article
Pages 347-350,350a,350b,351-365
Thomas D. Sargent, Peter H. Mathers

Chapter 20 Assays for Gene Function in Developing Xenopus Embryos Original Research Article
Pages 367-374,374a,374b,375-387
Peter D. Vize, Douglas A. Melton, Ali Hemmati-Brivanlou, Richard M. Harland

Chapter 21 Histological Preparation of Xenopus laevis Oocytes and Embryos Original Research Article
Pages 389-402,402a,402b,403-417
Gregory M. Kelly, Douglas W. Eib, Randall T. Moon

Chapter 22 Whole-Mount Staining of Xenopus and Other Vertebrates Original Research Article
Pages 419-428,428a,428b,429-441
Michael W. Klymkowsky, James Hanken

Chapter 23 In Situ Hyridization Original Research Article
Pages 443-463
Heather Perry O'Keefe, Douglas A. Melton, Beatriz Ferreiro, Chris Kintner

Chapter 24 DNA Recombination and Repair in Oocytes Eggs, and Extracts Original Research Article
Pages 467-486
Dana Carroll, Chris W. Lehman

Chapter 25 Expression of Ion Channels by Injection of mRNA into Xenopus Oocytes Original Research Article
Pages 487-509
Alan L. Goldin

Chapter 26 Tissue Culture of Xenopus Neurons and Muscle Cells as a Model for Studying Synaptic Induction Original Research Article
Pages 511-526
H. Benjamin Peng, Lauren P. Baker, Qiming Chen

Chapter 27 The Xenopus Embryo as a Model System for the Study of Cell—Extracellular Matrix Interactions Original Research Article
Pages 527-539
Douglas W. Desimone, Kurt E. Johnson

Chapter 28 Chromatin Assembly Original Research Article
Pages 541-559
Alan P. Wolffe, Caroline Schild

Chapter 29 DNA Replication in Cell-Free Extracts from Xenopus laevis Original Research Article
Pages 561-574,574a,574b,575-579
Gregory H. Leno, Ronald A. Laskey

Chapter 30 Cell Cycle Extracts Original Research Article
Pages 581-605
Andrew W. Murray

Chapter 31 Egg Extracts for Nuclear Import and Nuclear Assembly Reactions Original Research Article
Pages 607-634
Donald D. Newmeyer, Katherine L. Wilson

Chapter 32 Xenopus Cell Lines Original Research Article
Pages 635-654
J.C. Smith, J.R. Tata

Appendix A: Solutions and Protocols
Pages 657-662
H. Benjamin Peng

Appendix B: Injection of Oocytes and Embryos
Pages 663-669
Brian K. Kay

Appendix C: Mutants of Xenopus laevis
Pages 671-673
Anne Droin

Appendix D: Codon Usage Table for Xenopus laevis
Pages 675-677
J. Michael Cherry

Appendix E: Pictorial Collage of Embryonic Stages
Pages 679-681
Mike Danilchick, H. Benjamin Peng, Brian K. Kay

Appendix F: Xenopus Suppliers in the United States
Page 683

Appendix G: In Situ Hybridization: An Improved Whole-Mount Method for Xenopus Embryos
Pages 685-695
Richard M. Harland

Index
Pages 697-709

Contents of Recent Volumes
Pages 711-718