Visualizing RNA Dynamics in the Cell

Content: 
Series Page Page ii  
Copyright Page iv  
Contributors Pages xi-xiii  
Preface Pages xv-xvi Grigory S. Filonov, Samie R. Jaffrey 
Chapter One - RNA Imaging with Multiplexed Error-Robust Fluorescence In Situ Hybridization (MERFISH) Pages 1-49 J.R. Moffitt, X. Zhuang 
Chapter Two - Imaging Single mRNA Dynamics in Live Neurons and Brains Pages 51-64 H.C. Moon, H.Y. Park 
Chapter Three - Monitoring of RNA Dynamics in Living Cells Using PUM-HD and Fluorescent Protein Reconstitution Technique Pages 65-85 H. Yoshimura, T. Ozawa 
Chapter Four - Applications of Hairpin DNA-Functionalized Gold Nanoparticles for Imaging mRNA in Living Cells Pages 87-103 S.R. Jackson, A.C. Wong, A.R. Travis, I.E. Catrina, D.P. Bratu, D.W. Wright, A. Jayagopal 
Chapter Five - In Vivo RNA Visualization in Plants Using MS2 Tagging Pages 105-122 E.J. Peña, M. Heinlein 
Chapter Six - TRICK: A Single-Molecule Method for Imaging the First Round of Translation in Living Cells and Animals Pages 123-157 J.M. Halstead, J.H. Wilbertz, F. Wippich, T. Lionnet, A. Ephrussi, J.A. Chao 
Chapter Seven - Fluctuation Analysis: Dissecting Transcriptional Kinetics with Signal Theory Pages 159-191 A. Coulon, D.R. Larson 
Chapter Eight - IMAGEtags: Quantifying mRNA Transcription in Real Time with Multiaptamer Reporters Pages 193-213 J. Ray, I. Shin, M. Ilgu, L. Bendickson, V. Gupta, G.A. Kraus, M. Nilsen-Hamilton 
Chapter Nine - A Method for Expressing and Imaging Abundant, Stable, Circular RNAs In Vivo Using tRNA Splicing Pages 215-236 C.A. Schmidt, J.J. Noto, G.S. Filonov, A.G. Matera 
Chapter Ten - RNA-ID, a Powerful Tool for Identifying and Characterizing Regulatory Sequences Pages 237-253 C.E. Brule, K.M. Dean, E.J. Grayhack 
Chapter Eleven - Fluorescent Protein-Based Quantification of Alternative Splicing of a Target Cassette Exon in Mammalian Cells Pages 255-268 N.G. Gurskaya, D.B. Staroverov, K.A. Lukyanov 
Chapter Twelve - IRAS: High-Throughput Identification of Novel Alternative Splicing Regulators Pages 269-289 S. Zheng 
Chapter Thirteen - Analysis of Nonsense-Mediated mRNA Decay at the Single-Cell Level Using Two Fluorescent Proteins Pages 291-314 N.G. Gurskaya, A.P. Pereverzev, D.B. Staroverov, N.M. Markina, K.A. Lukyanov 
Chapter Fourteen - Developing Fluorogenic Riboswitches for Imaging Metabolite Concentration Dynamics in Bacterial Cells Pages 315-333 J.L. Litke, M. You, S.R. Jaffrey 
Author Index Pages 335-349  
Subject Index Pages 351-368