Viral Polymerases and Related Proteins

Content: 
Contributors to volume 275
Pages ix-xi

Preface
Pages xiii-xiv
Lawrence C. Kuo, David B. Olsen, Steven S. Carroll

Volumes in series
Pages xv-xxx

[1] Purification of viral polymerases: General considerations Original Research Article
Pages 3-16
Vinod Sardana, Mohinder Sardana

[2] Expression, purification, and characterization of the herpes simplex virus type-1 DNA polymerase Original Research Article
Pages 16-35
Paul E. Boehmer

[3] Assays for poliovirus polymerase, 3DPol, and authentic RNA replication in HeLa S10 extracts Original Research Article
Pages 35-57
David J. Barton, B. Joan Morasco, James B. Flanegan

[4] RNA-dependent RNA polymerase of hepatitis C virus Original Research Article
Pages 58-67
Raffaele De Francesco, Sven-Erik Behrens, Licia Tomei, Sergio Altamura, Josef Jiricny

[5] Characterization of coronavirus RNA polymerase gene products Original Research Article
Pages 68-89
Jens Herold, Stuart Siddell, John Ziebuhr

[6] Expression, purification, and characterization of orthomyxovirus: Influenza transcriptase Original Research Article
Pages 90-99
Joanne E. Tomassini

[7] Expression, purification, and characterization of rhabdovirus polymerase Original Research Article
Pages 99-122
Tapas Das, Bishnu P. De, Amiya K. Banerjee

[8] Expression and purification of retroviral HIV-1 reverse transcriptase Original Research Article
Pages 122-133
Mark W. Stahlhut, David B. Olsen

[9] Expression, purification, and characterization of DNA polymerases involved in papovavirus replication Original Research Article
Pages 133-167
Linda H. Malkas, Robert J. Hickey

[10] Heterologous expression, purification, and characterization of adenovirus DNA polymerase and preterminal protein Original Research Article
Pages 168-194
M. Ramachandra, Y. Sasaguri, R. Nakano, R. Padmanabhan

[11] Expression and characterization of hepadnavirus reverse transcriptases Original Research Article
Pages 195-208
Jianming Hu, Christoph Seeger

[12] Expression, purification, and characterization of vaccinia virus-encoded RNA and poly(A) polymerases Original Research Article
Pages 208-227
Paul Gershon, Bernard Moss

[13] Viral polymerase-associated 5′ → 3′-exonucleases: Expression, purification, and uses Original Research Article
Pages 227-238
Jon R. Sayers

[14] Isotopic assays of viral polymerases and related proteins Original Research Article
Pages 241-257
Robert D. Kuchta

[15] Nonisotopic assays of viral polymerases and related proteins Original Research Article
Pages 257-276
Josef Eberle, Charles Werner Knopf

[16] Catalytic activities associated with retroviral and viral polymerases Original Research Article
Pages 276-310
Sam Gabbara, James A. Peliska

[17] Approaches to high-volume screening assays of viral polymerases and related proteins Original Research Article
Pages 310-328
James L. Cole

[18] In situ DNA polymerase and RNase H activity gel assays as applied to hepadnavirus particles Original Research Article
Pages 328-347
Stephanie M. Oberhaus, John E. Newbold

[19] A system to analyze and identify inhibitors of HIV-1 gene regulation using a defective integrated provirus Original Research Article
Pages 348-361
Vera Byrnes, Daria Hazuda

[20] Preparation and use of synthetic oligoribonucleotides as tools for study of viral polymerases Original Research Article
Pages 365-382
Steven S. Carroll, Fritz Benseler, David B. Olsen

[21] Use of bacteriophage RNA polymerase in RNA synthesis Original Research Article
Pages 382-397
Vsevolod V. Gurevich

[22] Inhibition of viral polymerases by chain-terminating substrates: A kinetic analysis Original Research Article
Pages 398-424
Jeanne E. Wilson, David J.T. Porter, John E. Reardon

[23] Design of nucleoside analog inhibitors of herpesvirus polymerases Original Research Article
Pages 425-440
Ernest J. Prisbe, Ming S. Chen

[24] Development of nonnucleoside HIV reverse transcriptase inhibitors Original Research Article
Pages 440-470,IN1,471-472
Thomas J. Tucker, William C. Lumma, J. Christopher Culberson

[25] Analysis of inhibition of retroviral reverse transcriptase Original Research Article
Pages 472-502
Jan Balzarini, Erik De Clercq

[26] Novel methods of generating specific oligonucleotide inhibitors of viral polymerases Original Research Article
Pages 503-520
Hang Chen, David Brown, Larry Gold

[27] Analyzing the fidelity of reverse transcription and transcription Original Research Article
Pages 523-537
Jayne C. Boyer, Katarzyna Bebenek, Thomas A. Kunkel

[28] Site-directed mutagenic analysis of viral polymerases and related proteins Original Research Article
Pages 538-555
Paul L. Boyer, Stephen H. Hughes

[29] Analysis of resistance mutants of viral polymerases Original Research Article
Pages 555-600
John S. Oxford, Ali A. Al-Jabri, Christian A. Stein, Petros Levantis

Author index
Pages 601-641

Subject index
Pages 643-658