Viral Gene Techniques

Content: 
Contributors to volume 7
Pages ix-xiii

Preface
Page xv
Kenneth W. Adolph

Methods in molecular genetics
Page xvii

[1] Adeno-associated virus: Integrating vectors for human gene therapy Original Research Article
Pages 3-12
A.W. Skulimowski, R.J. Samulski

[2] Techniques for human adenovirus vector construction and characterization Original Research Article
Pages 13-30
Mary Hitt, Andrew J. Bett, Christina L. Addison, Ludvik Prevec, Frank L. Graham

[3] Construction and isolation of recombinant adenoviruses with gene replacements Original Research Article
Pages 31-44
David J. Spector, Lorna A. Samaniego

[4] Construction and use of recombinant vaccinia virus vectors Original Research Article
Pages 45-64
Douglas Grosenbach, Dennis E. Hruby

[5] Epstein-barr viral plasmid vectors and their amplifiable derivatives Original Research Article
Pages 65-86
Ann L. Kirchmaier, Tim A. Bloss, David Mackey, Bill Sugden

[6] Methods to study epstein-barr virus and p53 status in human cells Original Research Article
Pages 89-100
Alison J. Sinclair, Paul J. Farrell

[7] The SV40 minichromosome Original Research Article
Pages 101-113
Claudia Gruss, Rolf Knippers

[8] Site-specific integration of multigenic shuttle plasmids into the herpes simplex virus type 1 genome using a cell-free cre-lox recombination system Original Research Article
Pages 114-130
Siyamak Rasty, William F. Goins, Joseph C. Glorioso

[9] Strategies for studying transcription regulation of neurotropic viruses in the central nervous system: Lesson from JC virus Original Research Article
Pages 131-151
Jay Rappaport, Douglas A. Kerr, Kamel Khalili

[10] Analysis of the nuclear DNA-binding activity in cells overexpressing hepatitis B viral X and 3′ truncated S transactivators Original Research Article
Pages 152-166
Volker Schlüter, Wolfgang H. Caselmann

[11] Investigations on virus-host interactions: An abortive system Original Research Article
Pages 167-192
Christiane Zock, Walter Doerfler

[12] Quantitation of virus stocks produced from cloned human immunodeficiency virus DNA Original Research Article
Pages 195-208
Carol A. Deminie, Michael Emerman

[13] Quantitation of HIV-1 RNA in plasma or serum samples Original Research Article
Pages 209-217
V. Natarajan, N.P. Salzman

[14] Detection of cell fusion mediated by the envelopes of human retroviruses by transactivation of a reporter gene Original Research Article
Pages 218-236
Tatjana Dragic, Uriel Hazan, Marc Alizon

[15] Assembly of recombinant retroviral gag precursors into pseudovirions in the baculovirus-insect cell expression system Original Research Article
Pages 237-253
Gregory J. Tobin, Jane K. Battles, Lynn Rasmussen, Matthew A. Gonda

[16] Retrovirus reverse transcription and integration Original Research Article
Pages 254-266
Jonathan Leis, Ashok Aiyar

[17] In Vitro binding and transcription assays using the human T-cell leukemia virus type I tax protein Original Research Article
Pages 267-279
Mark G. Anderson, William S. Dynan, Maura-Ann H. Matthews

[18] Polymerase chain reaction-based techniques for utilizing retroviruses as cell lineage markers Original Research Article
Pages 280-295
Christopher A. Walsh

[19] New methods to study poliovirus assembly and encapsidation of genomic RNA Original Research Article
Pages 299-314
Casey D. Morrow, Donna C. Porter, David C. Ansardi

[20] Molecular studies of hepatitis delta virus RNAs Original Research Article
Pages 315-328
Tie-Bo Fu, Ting-Ting Wu, David Lazinski, John Taylor

[21] Systems to express recombinant RNA molecules by the influenza A virus polymerase in vivo Original Research Article
Pages 329-342
Ignacio Mena, Carmen Albó, Agustín Portela, Susana de la Luna, Javier Martín, Beatriz Perales, Amelia Nieto, Juan Ortín

[22] Analysis of membrane association in vivo and targeting of viral proteins in polarized epithelial cells Original Research Article
Pages 343-358
Debi P. Nayak, Amitabha Kundu, Christopher M. Sanderson

[23] Cloning of viral double-stranded RNA genomes by single primer amplification Original Research Article
Pages 359-372
Paul R. Lambden, Ian N. Clarke

[24] Using the RNA-capture assay to assess the RNA-binding activity of viral proteins Original Research Article
Pages 373-387
John T. Patton, Jian Hua

[25] Protein RNA-binding activity measured by northwestern blot analysis: The interferon-inducible RNA-dependent protein kinase PKR Original Research Article
Pages 388-403
Stephen J. McCormack, Charles E. Samuel

Erratum
Page 404

Index
Pages 405-416