Vascular Biology Protocols

Cover......Page 1
Frontmatter......Page 2
Rat Carotid Artery Balloon Injury Model......Page 13
Histological and Morphometric Analyses for Rat Carotid Balloon Injury Model......Page 43
Plaque Rupture Model in Mice......Page 79
Immunostaining of Mouse Atherosclerotic Lesions......Page 88
Surgical Animal Model of Ventricular Hypertrophy......Page 106
Animal Models of Hypertension......Page 116
Rat Models of Cardiac Insulin Resistance......Page 123
The Isolated, Perfused Pseudo-Working Heart Model......Page 154
Altering and Analyzing Glucose Metabolism in Perfused Hearts of Transgenic Mice......Page 160
Methods in the Evaluation of Cardiovascular Renin Angiotensin Aldosterone Activation and Oxidative Stress......Page 171
Detection and Quantification of Apoptosis in the Vasculature......Page 188
Endothelial Progenitor Cell and Mesenchymal Stem Cell Isolation, Characterization, Viral Transduction......Page 203
Assessment of Endothelial Damage/Dysfunction: A Focus on Circulating Endothelial Cells......Page 217
Evaluation of Endothelial Function by In Vivo Microscopy......Page 231
Haptotaxis of Endothelial Cell Migration Under Flow......Page 242
Isolation, Culture, and Functional Analysis of Adult Mouse Cardiomyocytes......Page 256
Mechanical Measurement of Contractile Function of Isolated Ventricular Myocytes......Page 268
Studying Ischemia and Reperfusion in Isolated Neonatal Rat Ventricular Myocytes Using Coverslip Hypoxia......Page 276
Isolation and Functional Studies of Rat Aortic Smooth Muscle Cells......Page 287
Detection of Reactive Oxygen Species and Nitric Oxide in Vascular Cells and Tissues......Page 297
Assessment of Protein Glycoxidation in Ventricular Tissues......Page 316
Assessment of PI-3 Kinase and Akt in Ischemic Heart Diseases in Diabetes......Page 332
Two-Dimensional Gel Electrophoresis in Platelet Proteomics Research......Page 342
Stem Cell Therapy in the Heart and Vasculature......Page 357
Index......Page 368