Two-dimensional DNA typing : a parallel approach to genome analysis

Content: Two-dimensional DNA typing: background and technology --
Analysis of DNA: general aspects --
Measuring DNA sequence variation --
DNA sequencing --
Restriction enzyme digestion: RFLP analysis --
Analysis of basepairing --
Evaluation --
Measuring DNA sequence variation on a total genome scale --
Two-dimensional separation of DNA fragments --
Enzymatic digestion as second dimension separation criterion --
DGGE in the second dimension --
Two-dimensional DNA typing --
Summary and discussion: general characteristics of 2D DNA typing. Genome analysis: general considerations --
Mapping genomes --
Physical mapping --
Genetic mapping --
DNA markers --
Micro- and minisatellite sequences --
Other interspersed repetitive sequences --
Total genome analysis with DNA markers --
Combining genetic and physical mapping --
Strategies for identifying genetic traits --
Role of two-dimensional DNA typing in genome analysis --
General methodology --
Methods --
DNA isolation --
DNA digestion and sample preparation --
Electrophoretic separations --
Electrotransfer of separation patterns. Hybridization analysis --
Experimental considerations --
Denaturing gradient gel constellation --
Buffer dependency of separation patterns --
Reproducibility --
Pattern evaluation by image analysis --
Image capturing --
Spot detection --
Calibration --
Matching --
Database generation, searching and matching --
Applications --
Genome analysis of lower organisms --
Analysis of viral and bacterial genomes --
Analysis of lower eukaryotes --
Genetic linkage analysis --
Locus-specific melting behaviour of minisatellite alleles --
Anatomy of a 2D DNA typing gel --
Association studies. Genetic analysis of cancer --
Mutation analysis --
Future developments --
Positional 2D DNA typing: the concerted use of genetic and physical mapping --
Two-dimensional cDNA typing: analysing the expressed part of the genome --
Gene scanning: diagnosis of all possible mutations in disease genes.