Tumor Microenvironment: Study Protocols

Front Matter....Pages i-viii
Staining Against Phospho-H2AX (γ-H2AX) as a Marker for DNA Damage and Genomic Instability in Cancer Tissues and Cells....Pages 1-10
Measuring DNA Replication in Hypoxic Conditions....Pages 11-25
Isolation of Proteins on Nascent DNA in Hypoxia and Reoxygenation Conditions....Pages 27-40
In Vivo Interrogation of the Hypoxic Transcriptome of Solid Tumors: Optimizing Hypoxic Probe Labeling with Laser Capture Microdissection for Isolation of High-Quality RNA for Deep Sequencing Analysis....Pages 41-58
Gas Chromatography Coupled to Mass Spectrometry (GC–MS) to Study Metabolism in Cultured Cells....Pages 59-88
Probing Cancer Cell Metabolism Using NMR Spectroscopy....Pages 89-111
Measuring the Impact of Microenvironmental Conditions on Mitochondrial Dehydrogenase Activity in Cultured Cells....Pages 113-120
Measuring Autophagy in the Context of Cancer....Pages 121-143
Methods for Studying Autophagy Within the Tumor Microenvironment....Pages 145-166
Methods: Using Three-Dimensional Culture (Spheroids) as an In Vitro Model of Tumour Hypoxia....Pages 167-196
Isolation of Glioma-Initiating Cells for Biological Study....Pages 197-209
Isolation of Mouse and Human Tumor-Associated Macrophages....Pages 211-229
Mouse Models of Brain Metastasis for Unravelling Tumour Progression....Pages 231-244
Quantification of Lung Metastases from In Vivo Mouse Models....Pages 245-251
A Multimodal Data Analysis Approach for Targeted Drug Discovery Involving Topological Data Analysis (TDA)....Pages 253-268
Efficient Protocol for the Identification of Hypoxic Cell Radiosensitisers....Pages 269-290
Back Matter....Pages 291-297