Tuberculosis : laboratory diagnosis and treatment strategies

Contents......Page 6
Contributors......Page 8
Abbreviations......Page 10
Introduction......Page 12
PART I – DIAGNOSIS......Page 14
1. Improving on Sputum Smear Microscopy for Diagnosis of Tuberculosis in Resource-poor Settings......Page 16
2. Molecular Diagnosis of Active Pulmonary Tuberculosis......Page 26
3. Improving on the LJ slope – Automated Liquid Culture......Page 47
4. Interferon-gamma Release Assays in the Diagnosis of Latent Tuberculosis Infection......Page 59
5. Measuring Tuberculosis Immune Responses in the Lung – The Correct Target?......Page 80
6. Sniffing Out Tuberculosis......Page 94
PART II – MEASURING RESISTANCE......Page 106
7. Role of Phenotypic Methods for Drug Susceptibility Testing of M. tuberculosis Isolates in the Era of MDR and XDR Epidemics......Page 108
8. Genotypic Measures of Antibiotic Susceptibility......Page 121
9. Molecular Tools for Fast Identification of Resistance and Characterization of MDR/XDR-TB......Page 138
PART III – UNDERSTANDING TREATMENT......Page 150
10. Monitoring Therapy by Bacterial Load......Page 152
11. Modelling Responses to Tuberculosis Treatment......Page 160
12. Measuring Gene Expression by Quantitative PCR (qPCR)......Page 177
13. Transcriptomic Approaches to Mapping Responses to Drug Therapy for Tuberculosis......Page 186
14. Mycobacterial Lipid Bodies and Resuscitation-promoting Factor Dependency as Potential Biomarkers of Response to Chemotherapy......Page 197
PART IV – TREATMENT STRATEGIES......Page 208
15. Clinical Trials in Tuberculosis Chemotherapy: The Challenges......Page 210
16. The Identification of 2-Aminothiazole-4-carboxylates (ATCs) as a New Class of Tuberculosis Agent: A Lesson in ‘HIT’ Identification......Page 219
17. Rifamycins Revisited......Page 232
18. Therapy of the XDR-TB Patient with Thioridazine – An Old Drug with New Applications......Page 245
19. Vaccines for Tuberculosis......Page 256
A......Page 274
D......Page 275
E......Page 276
I......Page 277
L......Page 278
M......Page 279
R......Page 280
S......Page 281
T......Page 282
Z......Page 283