Trichoderma reesei: Methods and Protocols

Front Matter ....Pages i-xi
 Ecological Genomics and Evolution of Trichoderma reesei  (Komal Chenthamara, Irina S. Druzhinina, Mohammad J. Rahimi, Marica Grujic, Feng Cai)....Pages 1-21
 Industrial Relevance of Trichoderma reesei as an Enzyme Producer  (Amanda J. Fischer, Suchindra Maiyuran, Debbie S. Yaver)....Pages 23-43
 The Potential of Synthetic Biology for Trichoderma reesei  (Roland Martzy, Astrid R. Mach-Aigner)....Pages 45-54
 Resistance Marker- and Gene Gun-Mediated Transformation of Trichoderma reesei  (Monika Schmoll, Susanne Zeilinger)....Pages 55-62
 Use of Auxotrophic Markers for Targeted Gene Insertions in Trichoderma reesei  (Irene Tomico-Cuenca, Christian Derntl)....Pages 63-72
 Open the Pores: Electroporation for the Transformation of Trichoderma reesei  (Franziska Wanka)....Pages 73-78
 Sexual Crossing of Trichoderma reesei  (Rita B. Linke)....Pages 79-85
 CRISPR/Cas9-Mediated Genome Editing of Trichoderma reesei  (Gen Zou, Zhihua Zhou)....Pages 87-98
 The Copper-Controlled RNA Interference System in Trichoderma reesei  (Lei Wang, Weixin Zhang, Xiangfeng Meng, Weifeng Liu)....Pages 99-111
 Batch Cultivation of Trichoderma reesei  (Birgit Jovanović)....Pages 113-118
 Image Analysis Method for the Characterization of Trichoderma reesei During Fermentations  (Nicolas Hardy, Maxime Moreaud, Fadhel Ben Chaabane)....Pages 119-133
 Measurement of Cellulase and Xylanase Activities in Trichoderma reesei  (Qing-Shan Meng, Fei Zhang, Chen-Guang Liu, Feng-Wu Bai, Xin-Qing Zhao)....Pages 135-146
 Flow Cytometry for Filamentous Fungi  (Matthias G. Steiger)....Pages 147-155
 Molecular Identification of Trichoderma reesei  (Mohammad J. Rahimi, Feng Cai, Marica Grujic, Komal Chenthamara, Irina S. Druzhinina)....Pages 157-175
 In Vivo Footprinting Analysis in Trichoderma reesei  (Alice Rassinger)....Pages 177-189
 RNA Characterization in Trichoderma reesei  (Petra Till)....Pages 191-235
 Proteomic Profiling of the Secretome of Trichoderma reesei  (So Fong Cam Ngan, Siu Kwan Sze)....Pages 237-249
 Transcriptomics in Trichoderma reesei  (Amanda C. C. Antonieto, Roberto N. Silva)....Pages 251-269
 The Comprehensive and Reliable Detection of Secondary Metabolites in Trichoderma reesei: A Tool for the Discovery of Novel Substances  (Bernhard Seidl, Christoph Bueschl, Rainer Schuhmacher)....Pages 271-295
 In Silico Gene Analysis and Oligonucleotide Design for the Construction of Expression Vectors  (Bernhard Seiboth)....Pages 297-309
 PacBio Long-Read Sequencing, Assembly, and Funannotate Reannotation of the Complete Genome of Trichoderma reesei QM6a  (Wan-Chen Li, Ting-Fang Wang)....Pages 311-329
 TSETA: A Third-Generation Sequencing-Based Computational Tool for Mapping and Visualization of SNPs, Meiotic Recombination Products, and RIP Mutations  (Hou-Cheng Liu, Wan-Chen Li, Ting-Fang Wang)....Pages 331-361
Back Matter ....Pages 363-364