Trichoderma: a Manual

Cover......Page 1
Trichoderma : Ganoderma Disease Control in Oil Palm: A Manual......Page 4
Copyright......Page 5
Contents......Page 8
Preface to manual: Trichoderma: Disease Control in Oil Palm......Page 10
Acknowledgements......Page 12
 1.1 Trichoderma – Biology, Life-cycle and Antagonism......Page 16
 1.2 Trichoderma as a Biological Control Agent......Page 18
 1.3 Basal Stem Rot Disease in Oil Palm......Page 19
 1.4 Causes of Basal Stem Rot Disease......Page 20
 1.5 Control of Basal Stem Rot caused by Ganoderma......Page 21
 1.6 Trichoderma as a Biological Control Agent for  Ganoderma......Page 24
  References......Page 26
 2.1 Health and Safety in the Laboratory......Page 32
 2.2 Health and Safety in the Nursery......Page 33
 2.4 On the Safety of Trichoderma spp. in Biocontrol Applications......Page 34
  Bibliography......Page 35
 3.1 Potato Sucrose Agar (PSA) Medium Preparation......Page 38
   Steps in preparing one litre of PSA medium......Page 39
 3.2 Maize Medium Preparation......Page 43
  Steps in making maize media......Page 44
  Steps in making culture medium......Page 46
  Bibliography......Page 49
  Equipment and tools needed for Trichoderma multiplication......Page 52
  Steps in Trichoderma multiplication on PSA......Page 53
  Materials, equipment and tools......Page 54
  Steps in Trichoderma multiplication on maize medium......Page 55
  Bibliography......Page 56
 5.1 Collection of Ganoderma Basidiocarps......Page 58
  Steps in basidiocarp collection......Page 59
 5.2 Ganoderma Culture Preparation......Page 61
  Steps in preparing one litre of PSA plus antibiotics medium......Page 62
  Steps in Ganoderma culture from basidiocarp to PSA medium......Page 64
  Steps in multiplying Ganoderma in culture......Page 66
 5.4 Preparation of Rubber Wood Blocks......Page 67
  Steps in the preparation of rubber wood blocks......Page 68
 5.5 Inoculation of Rubber Wood Blocks......Page 70
  Steps in the inoculation of rubber wood blocks......Page 71
 5.6 Incubation of Rubber Wood Blocks with Ganoderma......Page 72
  Steps in the incubation of rubber wood blocks......Page 73
 5.7 Pathogenicity Screening of Ganoderma Isolates......Page 74
  Bibliography......Page 75
 Steps in dual culture testing......Page 78
 Bibliography......Page 80
 7.1 Nursery Preparation......Page 82
 7.2 Selection of Inoculated Rubber Wood Blocks......Page 85
  Steps in selecting inoculated RWBs......Page 86
 7.3 Preparation of Trichoderma Isolates......Page 87
 7.4 Nursery Inoculation......Page 89
 7.5 Planting......Page 90
  Bibliography......Page 93
 8.1 External Observations......Page 96
  Steps in taking external observations......Page 97
  Steps in taking internal observations......Page 99
  Bibliography......Page 100
9 Production of Trichoderma for Commercial Application......Page 102
 Steps in commercial Trichoderma production......Page 103
 Bibliography......Page 104
 10.1 Trichoderma Application in the Field......Page 106
  Steps in Trichoderma application in the field......Page 107
 10.2 Post-application Monitoring......Page 110
  Bibliography......Page 111
Index......Page 114
Back Cover......Page 119