Transgenic Plants: Methods and Protocols

Front Matter ....Pages i-xiv
Front Matter ....Pages 1-1
 Repurposing Macromolecule Delivery Tools for Plant Genetic Modification in the Era of Precision Genome Engineering  (Qiudeng Que, Mary-Dell M. Chilton, Sivamani Elumalai, Heng Zhong, Shujie Dong, Liang Shi)....Pages 3-18
 The Use of an Automated Platform to Assemble Multigenic Constructs for Plant Transformation  (David G. J. Mann, Scott A. Bevan, Anthony J. Harvey, Rachelle A. Leffert-Sorenson)....Pages 19-35
 Ensifer-Mediated Transformation (EMT) of Rice (Monocot) and Oilseed Rape (Dicot)  (Dheeraj Singh Rathore, Evelyn Zuniga-Soto, Ewen Mullins)....Pages 37-48
 Setaria viridis as a Model Plant for Functional Genomic Studies in C4 Crops  (Polyana Kelly Martins, Bárbara Andrade Dias Brito da Cunha, Adilson Kenji Kobayshi, Hugo Bruno Correa Molinari)....Pages 49-65
 Transient Transformation Using Particle Bombardment for Gene Expression Analysis  (Andika Gunadi, Eric A. Dean, John J. Finer)....Pages 67-79
 Maize Transformation Using the Morphogenic Genes Baby Boom and Wuschel2  (Todd Jones, Keith Lowe, George Hoerster, Ajith Anand, Emily Wu, Ning Wang et al.)....Pages 81-93
 Efficient and Fast Production of Transgenic Rice Plants by Agrobacterium-Mediated Transformation  (Chuanyin Wu, Yi Sui)....Pages 95-103
 Protocol for Agrobacterium-Mediated Transformation and Transgenic Plant Production of Switchgrass  (QiuXia Chen, Guo-Qing Song)....Pages 105-115
 Biolistic Transformation of Wheat  (Bin Tian, Mónica Navia-Urrutia, Yueying Chen, Jordan Brungardt, Harold N. Trick)....Pages 117-130
 Mesophyll Protoplasts and PEG-Mediated Transfections: Transient Assays and Generation of Stable Transgenic Canola Plants  (Sareena Sahab, Matthew J. Hayden, John Mason, German Spangenberg)....Pages 131-152
 A Unified Agrobacterium-Mediated Transformation Protocol for Alfalfa (Medicago sativa L.) and Medicago truncatula  (Qingzhen Jiang, Chunxiang Fu, Zeng-Yu Wang)....Pages 153-163
 Poplar Transformation  (Tobias Bruegmann, Olaf Polak, Khira Deecke, Julia Nietsch, Matthias Fladung)....Pages 165-177
 The Genetic Transformation of Sweet Orange (Citrus sinensis L. Osbeck) for Enhanced Resistance to Citrus Canker  (Lorena Noelia Sendin, María Paula Filippone)....Pages 179-190
 Genetic Modification of Grapevine Embryogenic Cultures  (S. A. Dhekney, S. K. Sessions, M. Brungart-Rosenberg, C. Claflin, Z. T. Li, D. J. Gray)....Pages 191-201
 Agrobacterium-Mediated Transformation of Solanum tuberosum L., Potato  (Myron A. Bruce, Jessica L. Shoup Rupp)....Pages 203-223
 Agrobacterium tumefaciens-Mediated Transformation of Tomato  (Joyce Van Eck, Patricia Keen, Michelle Tjahjadi)....Pages 225-234
Front Matter ....Pages 235-235
 DNA Break Repair in Plants and Its Application for Genome Engineering  (Carla Schmidt, Michael Pacher, Holger Puchta)....Pages 237-266
 Gene Stacking in Plants Through the Application of Site-Specific Recombination and Nuclease Activity  (Vibha Srivastava)....Pages 267-277
 CRISPR/Cas9 for Mutagenesis in Rice  (Si Nian Char, Riqing Li, Bing Yang)....Pages 279-293
 Plant Biotechnology Applications of Zinc Finger Technology  (Stephen Novak)....Pages 295-310
Front Matter ....Pages 311-311
 Overview of Biotechnology-Derived Herbicide Tolerance and Insect Resistance Traits in Plant Agriculture  (Tejinder Mall, Manju Gupta, Tarlochan Singh Dhadialla, Sarria Rodrigo)....Pages 313-342
 Developing Transgenic Agronomic Traits for Crops: Targets, Methods, and Challenges  (John P. Davies, Cory A. Christensen)....Pages 343-365
 Transgenic and Genome Editing Approaches for Modifying Plant Oils  (Laura L. Wayne, Daniel J. Gachotte, Terence A. Walsh)....Pages 367-394
Front Matter ....Pages 395-395
 Molecular Analysis for Characterizing Transgenic Events  (Wei Chen, PoHao Wang)....Pages 397-410
 Detection of Transgenic Proteins by Immunoassays  (Satyalinga Srinivas Gampala, Bryant Wulfkuhle, Kimberly A. Richey)....Pages 411-417
 Systematic Evaluation of Field Crop Performance Using Modern Phenotyping Tools and Techniques  (Christopher R. Boomsma, Vladimir A. da Costa)....Pages 419-440
Back Matter ....Pages 441-444