Transgenic Mouse: Methods and Protocols

Front Matter ....Pages i-x
 The History of Transgenesis  (Thomas L. Saunders)....Pages 1-26
 Pronuclear Microinjection of One-Cell Embryos  (Melissa A. Larson)....Pages 27-33
 Integrase-Mediated Targeted Transgenics Through Pronuclear Microinjection  (Ruby Yanru Chen-Tsai)....Pages 35-46
 In Vivo Validation of CRISPR Reagents in Preimplantation Mouse Embryos  (Melissa A. Larson, Katelin A. Gibson, Jay L. Vivian)....Pages 47-57
 Targeted Mutations in the Mouse via Embryonic Stem Cells  (Marina Gertsenstein, Joffrey Mianné, Lydia Teboul, Lauryl M. J. Nutter)....Pages 59-82
 Blastocyst Microinjection with Embryonic Stem Cells  (Melissa A. Larson)....Pages 83-88
 Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos  (Bin Gu, Marina Gertsenstein, Eszter Posfai)....Pages 89-100
 Embryo Transfer Surgery  (Melissa A. Larson)....Pages 101-106
 Nonsurgical Embryo Transfer Protocol for Use with the NSET™ Device  (Barbara J. Stone)....Pages 107-111
 Nuclear Transfer and Cloning  (Ling Liu)....Pages 113-124
 Sperm-Mediated Genetic Modifications  (Marialuisa Lavitrano, Laura Farina, Maria Grazia Cerrito, Roberto Giovannoni)....Pages 125-132
 Genotyping Genetically Modified (GM) Mice  (Neeraj K. Aryal, Jan Parker-Thornburg)....Pages 133-148
 Nomenclature: Naming Your Gene-Modified Mouse  (Alicia Valenzuela)....Pages 149-162
 Breeding Strategies for Genetically Modified Mice  (Jan Parker-Thornburg)....Pages 163-169
 Strategies for Behaviorally Phenotyping the Transgenic Mouse  (Kenneth E. McCarson)....Pages 171-194
 Cryobanking and Recovery of Genetically Modified Mice  (Toru Takeo, Naomi Nakagata)....Pages 195-209
 Simple Transportation of Genetically Engineered Mice via Cold Storage Techniques  (Hidetaka Yoshimoto, Toru Takeo, Naomi Nakagata)....Pages 211-216
 Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells Using Sendai Virus  (Julia M. Draper, Jay L. Vivian)....Pages 217-234
Back Matter ....Pages 235-236