Transgenic Animals: Generation and Use

Cover
Half Title
Title Page
Copyright Page
Table of Contents
Preface
Contributors
Part I Overview
	1: Overview
Part II: The Techniques for Gene Transfer
	Section A: Gene Transfer into Mammal Embryos
		2: Transgenic Rats
		3: The Generation of Transgenic Rabbits
		4: Techniques and Problems in Producing Transgenic Pigs
		5: Gene Transfer into Goat Embryos
		6: The Generation of Transgenic Sheep by Pronuclear Microinjection
		7: Gene Microinjection into Bovine Pronuclei
		8: DNA Delivery by Cytoplasmic Injection
		9: The Direct Gene Transfer through Mammal Spermatozoa
	Section B: In Vitro Mammal Embryo Manipulation
		10: In Vitro Generation of One Cell Embryos in Sheep and Goat
		11: In Vitro Generation of Bovine Embryos
		12: The Use of Cryopreserved Fertilized Ova to Generate Transgenic Mammals
	Section C: Gene Transfer into Bird Embryos
		13: The Complete In Vitro Development of Quail Embryo
		14: The Microinjection of DNA into Early Chicken Embryo
		15: Production of Chimeric Chickens as Intermediates for Gene Transfer
		16: The Use of Retroviral Vectors for Gene Transfer into Bird Embryo
		17: Transgenic Chickens by Liposome-Sperm-Mediated Gene Transfer
		18: Ballistic Transfection of Avian Primordial Germ Cells In Ovo
	Section D: Gene Transfer into Fish and Lower Vertebrates
		19: Transgenic Salmonids
		20: Transferring Foreign Genes into Zebrafish Eggs by Microinjection
		21: Gene Injection into Xenopus Embryos
		22: The Techniques Using Electroporation to Generate Transgenic Fish
	Section E: Gene Transfer into Invertebrates
		23: Gene Transfer in Drosophila Melanogaster
		24: Genetic Transformation in Caenorhabditis Elegans
		25: Gene Transfer Technology in Marine Invertebrates
	Section F: The Use of ES Cells for Gene Transfer and Gene Targeting
		26: What are ES Cells?
		27: Formation of Mouse Chimeric Embryos from ES Cells
		28: Bovine Pluripotent Stem Cells for Transgenic Vectors
		29: Towards the Use of Primordial Germ Cells for Germline Modification
	Section G: The Direct Gene Transfer into Somatic Cells of Animals
		30: The Adenovirus Vectors for Gene Delivery
		31: The Development of Adeno-Associated Virus as a Vector for In Vivo Gene Therapy
		32: Advances Toward Synthetic Retro-Vectoring
		33: The Biolistic
Part III: The Fate of Microinjected DNA into Mammal Embryos
	Section A: The Integration and Modification of Foreign DNA
		34: The Fate of Microinjected Genes in Preimplantation Embryos
		35: Chromosomal Insertion of Foreign DNA
		36: Detection of Transgenes in the Preimplantation Stage Embryo
		37: Genetic Mosaicism in the Generation of Transgenic Mice
		38: Transgene Methylation in Transgenic Animals
		39: Influence of Genomic Imprinting on Transgene Expression
	Section B: The Vectors for Transgenesis
		40: The Use of Episomal Vectors for Transgenesis
		41: Insulation of Transgenes from Chromosomal Position Effects
		42: Transgene Rescue
		43: The Use of PI Bacteriophage Clones to Generate Transgenic Animals
		44: Bacterial Artificial Chromosomes and Animal Transgenesis
		45: The Use of Yeast Artificial Chromosomes for Transgenesis
		46: Gene Transfer Using Microdissected Chromosome Fragments
		47: Prospects for Directing Temporal and Spatial Gene Expression in Transgenic Animals
		48: Mouse Genetic Manipulation via Homologous Recombination
		49: Improvements in Transgene Expression by Gene Targeting
		50: Nuclear Antisense RNA as a Tool to Inhibit Gene Expression
		51: Controlled Genetic Ablation by Immunotoxin-Mediated Cell Targeting
		52: Reporter Genes and Detection of Mutational Activity in Mice
Part IV: The Use of Transg Enic Animals
	Section A: The Study of Gene Functions
		53: Gene Activity in the Preimplantation Mouse Embryo
		54: Insertional Mutagenesis in Transgenic Mice
		55: Enhancer-Trap Studies of the Drosophila Brain
		56: Transgenic Strategies for the Study of Mouse Development: An Overview
		57: Gene Transfer in Laboratory Fish: Model Organisms for the Analysis of Gene Function
		58: The Preparation of Human Antibodies from Mice Harbouring Human Immunoglobin Loci
	Section B: The Generation of Animal Models for Biomedical and Pharmaceutical Studies
		59: Standardization of Transgenic Lines: from Founder to an Established Animal Model
		60: The Use of Mouse Knockouts to Study Tumor Suppressor Genes
		61: The Generation of New Immortalized Cell Lines through Transgenesis
		62: The Use of Transgenic Mammals for AIDS Studies
		63: The Role of Mouse Models in the Development of New Therapies for Cystic Fibrosis
		64: Transgenic Animals in Atherosclerosis Research
		65: Genetically Engineered Mice in Obesity Research
		66: The Generation and Use of Transgenic Animals for Xenotransplantation
	Section C: The Preparation of Recombinant Proteins from Transgenic Animals
		67: The Preparation of Recombinant Proteins from Mouse and Rabbit Milk for Biomedical and Pharmaceutical Studies
		68: Production of Complex Human Pharmaceuticals in the Milk of Transgenic Goats Using the Goats Beta Casein Promoter
		69: Purification of Recombinant Proteins from Sheep\'s Milk
	Section D: The Modification of Milk Composition
		70: The Modification of Milk Protein Composition through Transgenesis: Progress and Problems
	Section E: The Protection of Animals Against Diseases
		71: The Use of Host-Derived Antiviral Genes
		72: The Use of Antisense RNA and Ribozyme Sequences to Prevent Infectious Diseases in Transgenic Animals
		73: Antibody Encoding Transgenes – Their Potential Use in Congenital and Intracellular Immunisation of Farm Animals
		74: Intracellular Antibodies (Intrabodies): Potential Applications in Transgenic Animal Research and Engineered Resistance to Pathogens
	Section F: Towards the Introduction of New Genetic Traits in Domestic Animals by Transgenesis
		75: Improved Wool Production from Insulin-Like Growth Factor 1 Targeted to the Wool Follicle in Transgenic Sheep
		76: The Transfer of Isolated Genes Having Known Functions
		77: Recent Progress in Mammalian Genomics and its Application for the Selection of Candidate Transgenes in Livestock Species
Part V: The New Problems Generated by Transgenic Animals
	78: To Save or Not to Save: The Role of Repositories in a Period of Rapidly Expanding Development of Genetically Engineered Strains of Mice
	79: TBASE: The Relationalized Database of Transgenic Animals and Targeted Mutations
	80: The Patenting of Transgenic Animals
	81: The Biosafety Problems of Transgenic Animals
	82: Food Safety Evaluation of Transgenic Animals
	83: From a Moral Point of View: Ethical Problems of Animal Transgenesis
Index