Tissue Culture. Methods and Applications

Content: 
Front Matter, Page iii
Copyright, Page iv
List of Contributors, Pages xv-xxii
Preface, Pages xxiii-xxv, PAUL F. KRUSE JR., M.K. PATTERSON JR.
Dr. Paul F. Kruse, Jr. (1921–1973), Pages xxvi-xxvii, M.K. PATTERSON JR.
Introduction to Primary Tissue Dissociation, Page 1
CHAPTER 1 - Pronase, Pages 3-5, Ralph B.L. Gwatkin
CHAPTER 2 - Trypsin: A. Mammalian Tissues, Pages 5-8, Charles Shipman Jr.
CHAPTER 2 - Trypsin: B. High Yield Method for Kidney Tissue, Pages 8-12, Héctor Montes de Oca
CHAPTER 2 - Trypsin: C. Marine Teleost Fish Tissues, Pages 12-14, M. Michael Sigel, Annie R. Beasley
CHAPTER 2 - Trypsin: D. Amphibian Tissues, Pages 14-15, Jerome J. Freed
CHAPTER 3 - Collagenase Treatment of Chick Heart and Thyroid, Pages 16-20, S. Robert Hilfer
CHAPTER 4 - Sequential Enzyme Treatment of Mouse Mammary Gland, Pages 21-24, F.J.A. Prop, G.J. Wiepjes
CHAPTER 5 - Nonenzymatic Dissociations: A. Leukocyte Cell Separation on Glass, Pages 25-29, Y. Rabinowitz
CHAPTER 5 - Nonenzymatic Dissociations: B. Specific Cell Fractionation on Chemically Derivatized Surfaces, Pages 29-36, Gerald M. Edelman
Introduction to Preparation of Primary Cultures, Page 37
CHAPTER 1 - Human Skin Fibroblasts, Pages 39-43, G.M. Martin
CHAPTER 2 - Fetal Human Diploid Cells, Pages 43-45, Leonard Hayflick
CHAPTER 3 - Human Mammary Tumors, Pages 45-50, E.Y. Lasfargues
CHAPTER 4 - Human Glia Cells, Pages 50-53, Jan Pontén
CHAPTER 5 - Human Liver Cells, Pages 54-58, M. Edward Kaighn
CHAPTER 6 - Human Blood Leukocytes, Pages 58-61, Paul S. Moorhead
CHAPTER 7 - Amniocentesis, Pages 62-64, Lewis L. Coriell
CHAPTER 8 - Human Lymph Nodes, Pages 65-69, Magdalena Eisinger
CHAPTER 9 - Human Biopsy Material from Genetic Abnormalities, Pages 69-72, Arthur E. Greene
CHAPTER 10 - Mammalian Myocardial Cells, Pages 72-81, Frederick H. Kasten
CHAPTER 11 - Mammalian Glomerular Cells, Pages 82-86, Leonard J. Quadracci, Gary E. Striker
CHAPTER 12 - Organotypic Mammalian Central and Peripheral Nerve Tissue, Pages 86-92, Murray B. Bornstein
CHAPTER 13 - Marmoset Bone Marrow, Pages 93-97, Raymond P. Porter, Nazareth Gengozian
CHAPTER 14 - Monkey Kidney Cells, Pages 97-101, Robert N. Hull, George Weaver, William R. Cherry
CHAPTER 15 - Rabbit Kidney and Skin, Pages 102-105, James G. Gallagher
CHAPTER 16 - Rat Skeletal Muscle Cells, Pages 106-114, David Yaffe
CHAPTER 17 - Rat Pituitary Explants, Pages 114-119, R.H. Kahn
CHAPTER 18 - Chick Embryo Cells, Pages 119-122, H. Rubin
CHAPTER 19 - Frog Embryos (Haploid Lines), Pages 123-128, Jerome J. Freed, Liselotte Mezger-Freed
CHAPTER 20 - Adult Amphibian Tissues and Leukocytes, Pages 129-133, D.E. Rounds
CHAPTER 21 - Marine Teleost Fish Tissues, Pages 133-135, M. Michael Sigel, Annie R. Beasley
CHAPTER 22 - Fish Lymphocytes and Blastogenesis, Pages 135-138, M. Michael Sigel, E. Churchill McKinney, John C. Lee
CHAPTER 23 - Freshwater Fishes, Pages 138-143, Ken Wolf
CHAPTER 24 - Goldfish Tissues, Pages 143-146, Laura S. McKenzie, N.G. Stephenson
CHAPTER 25 - Reptiles, Pages 147-150, H Fred Clark
CHAPTER 26 - Dipteran Embryos and Larvae (Diploid Lines), Pages 150-152, Imogene Schneider
CHAPTER 27 - Cockroach and Grasshopper Embryo Tissue, Pages 153-156, E.P. Marks
CHAPTER 28 - Plants: Haploid Tissue Cultures, Pages 157-161, I.K. Vasil
CHAPTER 29 - Aspen Callus, Pages 161-165, L.L. Winton, M.C. Mathes
CHAPTER 30 - Plant Embryos, Pages 166-170, John G. Torrey
CHAPTER 31 - Somatic Plant Cells, Pages 170-172, T. Murashige
CHAPTER 32 - Roots, Pages 173-178, H.E. Street
CHAPTER 33 - Leaves, Pages 179-181, T.A. Steeves
Introduction to Cell Harvesting, Page 183
CHAPTER 1 - Pronase, Pages 185-188, John F. Foley, Byron Th. Aftonomos
CHAPTER 2 - Trypsinization of Diploid Human Fibroblasts, Pages 188-192, Jack Litwin
CHAPTER 3 - Transplantable Rat Tumors and Cold Temperature Release, Pages 192-194, M.K. Patterson Jr.
CHAPTER 4 - Cells at Defined Stages of the Cell Cycle: A. Selection of Mitotic Cells from Monolayer Culture, Pages 195-198, S. Shall
CHAPTER 4 - Cells at Defined Stages of the Cell Cycle: B. Sedimentation in Sucrose and Ficoll Gradients of Cells Grown in Suspension Culture, Pages 198-204, S. Shall
CHAPTER 5 - Processing Cells for Enzyme Assays, Pages 204-207, V.J. Cristofalo, Joan Kabakjian
Introduction to Replicate Culture Methods, Page 209
CHAPTER 1 - Monolayer Cultures, Pages 211-215, Virginia J. Evans, William G. Taylor
CHAPTER 2 - Plant Cell Suspension Cultures, Pages 215-219, A.C. Hildebrandt
CHAPTER 3 - Subculturing Human Diploid Fibroblast Cultures, Pages 220-223, Leonard Hayflick
CHAPTER 4 - Apparatus for Changing Tissue Culture Media, Pages 224-226, D.O. Cliver
CHAPTER 5 - Single “Subculture” in Situ Using Glass Beads, Pages 226-230, G.M. Kolodny
CHAPTER 6 - Maintenance of Diploid Fibroblast Cultures as Nonmitotic Populations, Pages 231-233, R.T. Dell'Orco, J.G. Mertens, P.F. Kruse Jr.
Introduction to Single Cell Isolations and Cloning, Page 235
CHAPTER 1 - Animal Cells: A. Capillary Techniques, Pages 237-241, K.K. Sanford
CHAPTER 1 - Animal Cells: B. Microdrop Techniques, Pages 241-244, Ian Macpherson
CHAPTER 2 - Plant Cells, Pages 244-254, A.C. Hildebrandt
CHAPTER 3 - Dilution Plating and Nutritional Considerations: A. Animal Cells, Pages 254-261, Richard G. Ham
CHAPTER 3 - Dilution Plating and Nutritional Considerations: B. Plant Cells, Pages 261-264, D.K. Dougall
CHAPTER 4 - Dilution Plating on Coverslip Fragments, Pages 264-266, G.M. Martin
CHAPTER 5 - Microtest Plates: A. Single Cell Clones, Pages 266-269, John E.K. Cooper
CHAPTER 5 - Microtest Plates: B. Replica Plating, Pages 270-274, James A. Robb
CHAPTER 6 - Low Oxygen Tension Technique, Pages 274-276, Alan Richter
CHAPTER 7 - Soft Agar Techniques, Pages 276-280, Ian Macpherson
Introduction to Perfusion and Mass Culture Techniques, Page 281
CHAPTER 1 - Dual-Rotary Circumfusion System, Pages 283-291, George G. Rose
CHAPTER 2 - Automated Single Rose Chamber for Cardiac and Other Cells, Pages 291-297, Frederick H. Kasten
CHAPTER 3 - Culture Dish Perfusion with Cinemicrography, Pages 298-303, LaRoy N. Castor
CHAPTER 4 - Sykes-Moore Chamber, Pages 303-308, J.A. Sykes
CHAPTER 5 - Culture of the Lens (33°), Pages 309-316, Bernard Schwartz
CHAPTER 6 - Cardiac Cells in a Capillary Tube, Pages 316-321, M.A. Savageau
CHAPTER 7 - Artificial Capillaries: An Approach to Tissue Growth in Vitro, Pages 321-327, R.A. Knazek, P.M. Gullino
CHAPTER 8 - Replicate Roller Bottles, Pages 327-331, W.L. Whittle, P.F. Kruse Jr.
CHAPTER 9 - Replicate Tube Chambers for Bones, Pages 331-333, J. Bornstein, P.L. Schwartz, R.E.H. Wettenhall
CHAPTER 10 - Multisurface Stacked Plate Propagators, Pages 333-338, Joseph B. Schleicher
CHAPTER 11 - Bulk Culture of Cell Monolayers, Pages 338-344, William House
CHAPTER 12 - Spin Filter Device for Suspension Cultures, Pages 345-351, Philip S. Thayer
CHAPTER 13 - Automation of Continuous Suspension Cultures by Means of a Nephelostat, Pages 351-358, Carl Peraino, William J. Eisler Jr.
CHAPTER 14 - A Chemostat For Single Cell Cultures of Higher Plants, Pages 359-363, W.G.W. Kurz
Introduction to Cell Propagation on Miscellaneous Culture Supports, Page 365
CHAPTER 1 - Collagen-Coated Cellulose Sponge, Pages 367-371, Joseph Leighton
CHAPTER 2 - Microcarrier Cultures of Animal Cells, Pages 372-377, A.L. van Wezel
CHAPTER 3 - Polyester Sheeting, Pages 378-379, H. Firket
CHAPTER 4 - Protein Polymers as a Substratum for the Modulation of Cell Proliferation in Vitro, Pages 379-383, A. Macieira-Coelho, S. Avrameas
CHAPTER 5 - Titanium Disks, Pages 383-387, Jack Litwin
CHAPTER 6 - Filter Paper Supports for Plant Cultures, Pages 387-392, René Heller
Introduction to Evaluation of Culture Dynamics, Page 393
CHAPTER 1 - Hemocytometer Counting, Pages 395-397, Marlene Absher
CHAPTER 2 - Electronic Enumeration and Sizing of Cells: A. Primary Tissue Cells, Pages 398-399, W.F. Daly
CHAPTER 2 - Electronic Enumeration and Sizing of Cells: B. Tissue Culture Cells, Pages 400-405, M. Harris
CHAPTER 3 - Dye Exclusion Tests for Cell Viability, Pages 406-408, Hugh J. Phillips
CHAPTER 4 - Quantitative Methods for Measuring Cell Growth and Death, Pages 408-412, H.T. Holden, W. Lichter, M.M. Sigel
CHAPTER 5 - Cell Cycle Analysis: A. Mammalian Cells, Pages 412-422, A. Macieira-Coelho
CHAPTER 5 - Cell Cycle Analysis: B. Control and Analysis of the Mitotic Cycle in Cultured Plant Root Meristems, Pages 423-428, J. Van't Hof
Introduction to Recent Techniques Facilitating Microscopic Observation of Cells, Page 429
CHAPTER 1 - Contrast Methods in the Microscopy of Living Tissue, Pages 431-436, Robert F. Smith
CHAPTER 2 - Rapid Embedding of Cell Culture Monolayers and Suspensions, Pages 436-437, E. Robbins
CHAPTER 3 - Embedding in Situ, Pages 438-443, B.R. Brinkley, Jeffrey P. Chang
CHAPTER 4 - Vertical Sectioning: A. Cells on Millipore Filters, Pages 443-448, H. Dalen
CHAPTER 4 - Vertical Sectioning: B. Cells in Plastic Flasks, Pages 448-451, L.N. Keen, R. Reynolds, W.L. Whittle, P.F. Kruse Jr.
CHAPTER 4 - Vertical Sectioning: C. Cells Grown on Decalcified Eggshell Membrane with Special Reference to Electron Microscopic Study, Pages 452-458, Joseph Leighton, Nabil Abaza, Sunder Mansukhani
CHAPTER 5 - Hydraulically Operated Microscope Stage, Pages 458-462, Frederick H. Kasten
CHAPTER 6 - Culture Dish for High Resolution Microscopy, Pages 463-466, A.D. Deitch, A.F. Miranda, G.C. Godman
Introduction to Cell Hybridization, Page 467
CHAPTER 1 - Somatic Hybridization in Studies of Heredity of Cell Malignancy, Pages 469-475, Georges Barski
CHAPTER 2 - Production and Characterization of Proliferating Somatic Cell Hybrids, Pages 475-500, Richard E. Giles, Frank H. Ruddle
CHAPTER 3 - Plant Protoplast Culture, Pages 500-505, R.A. Miller, K.N. Kao, O.L. Gamborg
Introduction to Virus Propagation and Assay, Page 507
CHAPTER 1 - Microculture Procedures: A. Vesicular Stomatitis Virus, Pages 509-516, Leonard J. Rosenthal, Isaac L. Shechmeister
CHAPTER 1 - Microculture Procedures: B. Simian Virus 40, Pages 517-524, James A. Robb
CHAPTER 1 - Microculture Procedures: C. Antibody Neutralization Surveys, Pages 524-527, S.S. Kalter
CHAPTER 2 - Quantal and Enumerative Titration of Virus in Cell Cultures, Pages 527-532, C.H. Cunningham
CHAPTER 3 - Diagnosis of Virus-Infected Cultures with Fluorescein-Labeled Antisera, Pages 532-535, G. Shramek, L. Falk Jr.
CHAPTER 4 - Rapid Method for Detection of Budding Virus by Electron Microscopy, Pages 535-539, B. Kramarsky
CHAPTER 5 - Monitoring for Presence of Oncogenic Virus in Tissue Culture, Pages 539-548, A.J. Hackett
CHAPTER 6 - Tobacco Mosaic Virus in Plant Tissue Culture, Pages 549-558, A.C. Hildebrandt
Introduction to Production of Hormones and Intercellular Substances, Page 559
CHAPTER 1 - Growth Hormone and Prolactin from Rat Pituitary Tumor Cells, Pages 561-569, P.S. Dannies, A.H. Tashjian Jr.
CHAPTER 2 - Enhancement of Growth Hormone Production by Cortisol, Pages 570-575, P.O. Kohler, W.E. Bridson
CHAPTER 3 - Human Chorionic Gonadotropin, Pages 575-578, R.A. Pattillo
CHAPTER 4 - Parathyroid Hormone and Calcitonin, Pages 578-586, K.M. Gautvik, A.H. Tashjian Jr.
CHAPTER 5 - Measurement of Collagen Synthesis, Pages 586-588, B. Goldberg
CHAPTER 6 - Evaluation of Milk Protein Synthesis, Pages 589-593, B.L. Larson, Corine R. Andersen
CHAPTER 7 - Interferon Yield Versus Cell Genotype, Pages 593-599, J.M. Moehring, W.R. Stinebring
CHAPTER 8 - Immunoglobulins, Pages 599-607, Yuji Matsuoka
Introduction to Diagnosis and Understanding of Disease, Page 609
CHAPTER 1 - Some Cell Culture Procedures in Diagnostic Medical Virology, Pages 611-617, Margaret A.J. Moffat
CHAPTER 2 - Diagnostic Use of Cell Cultures Initiated from Amniocentesis, Pages 617-622, Carlo Valenti
CHAPTER 3 - Expressions of Citrullincmia in Skin Fibroblast Cultures, Pages 623-626, T.A. Tedesco, W.J. Mellman
CHAPTER 4 - The Hurler Cell in Culture, Pages 627-633, B. Shannon Danes
CHAPTER 5 - Characteristics of Fibroblasts Cultured from Patients with Galactosemia or Galactokinase Deficiency, Pages 633-638, Robert S. Krooth, Elizabeth K. Sell
CHAPTER 6 - Evaluation of Amino Acid Metabolism in Maple Syrup Urine Disease, Pages 639-643, Joseph Dancis, Joel Hutzler, Rody P. Cox
CHAPTER 7 - Malignant Transformation of Cells in Culture Using Oncogenic Chemicals, Pages 644-653, Charles Heidelberger, Catherine A. Reznikoff, David F. Krahn
CHAPTER 8 - Malignant Transformation of Cells in Culture, Pages 653-658, F. Rapp
CHAPTER 9 - Some in Vitro Models of Normal and Malignant Cell Interaction, Pages 659-664, G. Barski, J. Belehradek Jr.
CHAPTER 10 - Growth and Isolation of Nutritional Variants, Pages 665-667, M.K. Patterson Jr., E. Conway
Introduction to Quality Control Measures, Page 669
CHAPTER 1 - Glassware Preparation, Sterilizations, and Use of Laminar Flow Systems, Pages 671-673, Lewis L. Coriell
CHAPTER 2 - Purification and Standardization of Water for Tissue Culture, Pages 674-677, R.W. Pumper
CHAPTER 3 - A Surveillance Procedure Applied to Sera, Pages 677-682, Charles W. Boone
CHAPTER 4 - Preparation of Sera for Nutritional Studies, Pages 682-686, M.K. Patterson Jr., M.D. Maxwell
CHAPTER 5 - Sample Preparations of Media: A. Vertebrate Cells, Pages 686-692, Helen J. Morton, Joseph F. Morgan
CHAPTER 5 - Sample Preparations of Media: B. Invertebrate Cells, Pages 692-697, James L. Vaughn
CHAPTER 5 - Sample Preparations of Media: C. Plant Cultures, Pages 698-703, T. Murashige
CHAPTER 6 - Determination and Survey of Osmolality in Culture Media, Pages 703-709, Charity Waymouth
CHAPTER 7 - Control of Culture pH with Synthetic Buffers, Pages 709-712, Charles Shipman Jr.
CHAPTER 8 - Freezing, Storage, and Recovery of Cell Stocks, Pages 712-718, John E. Shannon, Marvin L. Macy
CHAPTER 9 - Detection, Elimination, and Prevention of Bacteria and Fungi in Tissue Cultures, Pages 718-721, Lewis L. Coriell
CHAPTER 10 - Screening Tissue Cultures for Mycoplasma Infections, Pages 722-728, Leonard Hayflick
CHAPTER 11 - Mycoplasma Contamination of Cell Cultures: Incidence, Source, Prevention, and Problems of Elimination, Pages 729-735, Michael F. Barile
CHAPTER 12 - Elimination of Pathogens from Shoot Tip Cultures, Pages 735-739, Ralph Baker, Harold Kinnaman
CHAPTER 13 - Contamination of Tissue Culture and Its Cure: Some Examples, Pages 739-744, W. House
CHAPTER 14 - Serological Identifications of Cells in Culture: A. Animal Cells by Fluorescent-Labeled Antibody, Pages 744-749, C.S. Stulberg, W.F. Simpson
CHAPTER 14 - Serological Identifications of Cells in Culture: B. Poikilotherm Cell Identity by Cytotoxic Antibody Test, Pages 750-753, Arthur E. Greene, Jesse Charney
CHAPTER 14 - Serological Identifications of Cells in Culture: C. Invertebrate Cell Cultures, Pages 753-758, Arthur E. Greene, Jesse Charney
CHAPTER 14 - Serological Identifications of Cells in Culture: D. Detection of HL-A Antigens on Cultured Normal Human Diploid Fibroblasts, Pages 758-763, Chaim Brautbar
CHAPTER 15 - Karyology of Cells in Culture: A. Preparation and Analysis of Karyotypes and Idiograms, Pages 764-767, T.C. Hsu
CHAPTER 15 - Karyology of Cells in Culture: B. Harvesting Human Leukocyte Cultures for Chromosome Cytology, Pages 768-773, P.S. Moorhead
CHAPTER 15 - Karyology of Cells in Culture: C. Constitutive Heterochromatin (C-Bands), Pages 773-777, Frances E. Arrighi
CHAPTER 15 - Karyology of Cells in Culture: D. Fluorescent Banding of Chromosomes (Q-bands), Pages 778-781, C.C. Lin, Irene A. Uchida
CHAPTER 15 - Karyology of Cells in Culture: E. Trypsin Technique to Reveal G-Bands, Pages 782-787, H.C. Wang, S. Fedoroff
CHAPTER 15 - Karyology of Cells in Culture: F. Characteristics of Insect Cells, Pages 788-790, Imogene Schneider
CHAPTER 15 - Karyology of Cells in Culture: G. Characteristics of Plant Cells, Pages 791-797, Carl R. Partanen
CHAPTER 16 - Biochemical Identification of Cells in Culture: A. Human Cell Lines by Enzyme Polymorphism, Pages 797-804, S.M. Gartler, R.A. Farber
CHAPTER 16 - Biochemical Identification of Cells in Culture: B. Enzymatic “Fingerprinting”, Pages 804-807, John E. Shannon, Marvin L. Macy
CHAPTER 17 - Introduction to Cytoenzymological Methods and Isozymes, Pages 808-821, P.J. Melnick
CHAPTER 18 - Transport and Shipping of Cultures, Including International Regulations, Pages 822-827, Leonard Hayflick
Author Index, Pages 829-851
Subject Index, Pages 852-868