Therapeutic Monoclonal Antibodies: From Bench to Clinic

THERAPEUTIC MONOCLONAL ANTIBODIES......Page 5
CONTENTS......Page 7
FOREWORD......Page 11
PREFACE......Page 13
CONTRIBUTORS......Page 15
PART I ANTIBODY BASICS......Page 19
1. Therapeutic Monoclonal Antibodies: Past, Present, and Future......Page 21
2. Antibody Molecular Structure......Page 69
3. Glycosylation of Therapeutic IgGs......Page 85
4. Antibody Databases and Tools: The IMGT(®) Experience......Page 109
PART II ANTIBODY SOURCES......Page 133
5. Human Antibodies from Transgenic Mice......Page 135
6. Rabbit Hybridoma......Page 169
7. Human Antibody Repertoire Libraries......Page 187
PART III IN VITRO DISPLAY TECHNOLOGY......Page 207
8. Antibody Phage Display......Page 209
9. Yeast Surface Display......Page 231
10. Ribosomal Display......Page 257
11. Bacterial Display of Antibodies......Page 273
12. Antibody Selection from Immunoglobulin Libraries Expressed in Mammalian Cells......Page 301
PART IV ANTIBODY ENGINEERING......Page 327
13. Antibody Engineering: Humanization, Affinity Maturation, and Selection Techniques......Page 329
14. Modulation of Serum Protein Homeostasis and Transcytosis by the Neonatal Fc Receptor......Page 353
15. Engineering the Antibody Fc Region for Optimal Effector Function......Page 367
PART V PHYSIOLOGY AND IN VIVO BIOLOGY......Page 389
16. Antibody-Complement Interaction......Page 391
17. Bacteria Immunoglobulin-Binding Proteins: Biology and Practical Applications......Page 403
18. Immunogenicity Screening Using in Silico Methods: Correlation between T-Cell Epitope Content and Clinical Immunogenicity of Monoclonal Antibodies......Page 435
19. Monoclonal Antibody Pharmacokinetics and Pharmacodynamics......Page 457
20. Biodistribution and Imaging......Page 479
21. Antibodies and the Blood-Brain Barrier......Page 501
PART VI ANTIBODY CHARACTERIZATION......Page 521
22. Determination of Equilibrium Dissociation Constants......Page 523
23. Molecular and Functional Characterization of Monoclonal Antibodies......Page 543
24. Characterization of Heterogeneity in Monoclonal Antibody Products......Page 559
PART VII ANTIBODY EXPRESSION......Page 573
25. Antibody Expression in Mammalian Cells......Page 575
26. Production of Antibodies in Pichia pastoris......Page 591
27. Production of Antibody Fab\' Fragments in E. coli......Page 607
28. Production of Human Therapeutic Monoclonal Antibodies in Chicken Eggs......Page 641
29. Production of Antibodies in Plants......Page 669
PART VIII THERAPEUTIC ANTIBODIES......Page 691
30. The Formulation and Delivery of Monoclonal Antibodies......Page 693
31. Therapeutic Antibodies in Clinical Use and Leading Clinical Candidates......Page 729
32. Follow-On Protein Products: What, Where, When, How?......Page 781
33. Monomeric Fc Fusion Molecules......Page 797
34. Radioimmunotherapy: Current Status and Future Directions......Page 815
35. Antibody-Drug Conjugate Therapy......Page 839
ABBREVIATIONS......Page 875
INDEX......Page 883