The Sodium Pump: Structure Mechanism, Hormonal Control and its Role in Disease

Front Matter....Pages I-XIX
Transcription factors regulating the Na + /K + -ATPase genes....Pages 1-10
Expression of Functional Na + /K + -ATPase in Yeast....Pages 11-20
Expression of Functional Na + /K + -ATPase in Insect Cells Using Baculovirus....Pages 21-32
Sp1 and an E-Box Binding Protein Regulate Na + /K + -ATPase α2-Subunit Gene....Pages 33-36
Cloning and Analysis of the 5’-Flanking Region of the Rabbit Na + /K + -ATPase α2 Subunit Gene....Pages 37-40
Positive and Negative Transcriptional Elements in the Chicken Na + /K + -ATPase α1-Subunit Gene....Pages 41-44
Transcriptional Regulation of Na + /K + -ATPase α1 Gene Expression by 8-Bromo-Cyclic AMP in Renal Cells....Pages 45-48
Cloning of the Dog Na + /K + -ATPase α1-Subunit....Pages 49-52
Cloning and Characterization of a New Member of the Family of Na + /K + -ATPase Genes....Pages 53-56
The Cloning of Homologous Na + /K + -ATPase Genes from New Species....Pages 57-60
Functional expression of the gastric H,K-ATPase in Xenopus oocytes....Pages 61-65
Over-expression of the peptide containing the ATP-binding site in Na/K-ATPase α-subunit....Pages 66-69
Expression of Na + ,K + -ATPase in Saccharomyces cerevisiae ....Pages 70-73
Regulation of Na + /K + -ATPase α1 and ß1 Expression in Epithelial Cells....Pages 74-77
The α-Subunit of the Na + /K + -ATPase has Catalytic Activity Independent of the ß-subunit....Pages 78-81
Functional Expression of Na + /K + -ATPase α and β Isoforms....Pages 82-85
Ca 2+ /Calmodulin-Sensitive Na + /K + -ATPase Activity Expressed in Chimeric Molecules between the Plasma Membrane Ca 2+ -ATPase and the Na + /K + -ATPase α-Subunit....Pages 86-89
Expression of Na + /K + -ATPase and H + /K + -ATPase fusion proteins in Sf -9 insect cells....Pages 90-93
In Vitro Synthesis of H + /K + -ATPase Polypeptides in Insect Cells Using the Baculovirus Expression System....Pages 94-97
Functional domains of sarcoplasmic reticulum Ca 2+ -ATPase studied by site-directed mutagenesis....Pages 98-109
Conservation Patterns Define Interactions Between Transmembrane Helices of P-type Ion Pumps....Pages 110-119
Structural Studies of Ca 2+ -ATPase by Cryoelectron Microscopy....Pages 120-130
The Topology of Sarcoplasmic Reticulum Ca 2+ -ATPase and Na + ,K + -ATPase in the M5/M6 Region....Pages 131-134
Changes of molecular structure and interaction in the catalytic cycle of sarcoplasmic reticulum Ca 2+ - ATPase....Pages 135-138
Ca 2+ binding to sarcoplasmic reticulum Ca 2+ -ATPase in the absence of chelators....Pages 139-142
Electrogenicity and Countertransport Properties of Reconstituted Sarcoplasmic Reticulum Ca 2+ -ATPase....Pages 143-146
Endo- Exocytotic Sorting of Na + /K + -Pumps in Developing Oocytes and Embryos of Xenopus laevis ....Pages 147-158
Determinants of Cell Surface Distributions of Na + /K + ATPase in Polarized Epithelial Cells....Pages 159-169
Physiologic Relevance of the α2 Isoform of Na,K-ATPase in Muscle and Heart....Pages 170-180
Na + /K + -ATPase of Neurons and Glia....Pages 181-191
Expression of a Truncated α Subunit of the Na + ,K + -ATPase (α-1-T) in Smooth Muscle Cells....Pages 192-199
The C-Terminus of Na + ,K + -ATPase β-Subunit Contains a Hydrophobic Assembly Domain....Pages 200-202
Synthesis and secretion of the extracellular domain of the β1 subunit isoform of NA + /K + -ATPase into the culture medium of CHO cells....Pages 203-205
The Amino-terminal Transmembrane Region of the β Subunit is Important for Na + /K + -ATPase Complex Stability....Pages 206-209
Construction and Expression of Chimeric cDNAs of Na + /K + and H + /K + -ATPase β-subunits....Pages 210-213
Segregation of Maternal Na + /K + -pump α1 and β3 mRNAs to Animal Blastomeres of Early Xenopus Embryos....Pages 214-217
Isoform Specific Antisera for the Na + /K + -ATPase β1 and β2 Subunits: Localization in Fetal Rat Tissues....Pages 218-221
Polyclonal Antibodies to Extramembrane Domains of Na + /K + -ATPase α1 and α3 Isoforms....Pages 222-225
Differential Axonal Transport of Individual (Na,K)-ATPase α Subunit Isoforms in Rat Sciatic Nerve....Pages 226-229
Differential Expression of Na + /K + -ATPase Isoforms in Cultured Rodent Cardiac and Skeletal Muscle Cells....Pages 230-233
Na,K-ATPase Abundance and Activity in HeLa Cells Transfected with Rat α Isoforms....Pages 234-237
Aldosterone Induces a Delayed Parallel Increase in Total and Cell-Surface Na + /K + -ATPase in A6 Kidney Cells....Pages 238-241
Differential aldosterone effects on two epithelial cell pools of [ 3 H]ouabain binding sites....Pages 242-245
Branchial Na + /K + -ATPase Expression in the European Eel (Anguilla anguilla) following Saltwater Acclimation....Pages 246-249
Differentiation of Na + /K + -ATPase by digoxigenin-labelled lectins on blots. Evidence for organ-typical glycoforms....Pages 250-253
Structural Requirements for the Interaction between the Na + /K + -ATPase α - and β-Subunits....Pages 254-263
Structure and Function of Ion Pumps Studied by Atomic Force Microscopy and Gene-transfer Experiments Using Chimeric Na + /K + - and Ca 2+ ATPases....Pages 264-275
Na,K-ATPase: Cardiac Glycoside Binding and Functional Importance of Negatively Charged Amino Acids of Transmembrane Regions....Pages 276-286
Functional Analysis of the Disulfide Bonds of Na + /K + -ATPase β-subunit by Site-directed Mutagenesis....Pages 287-296
Purified Renal Na + /K + -ATPase; Subunit Structure and Structure-Function Relationships of the N-Terminus of the α1- Subunit....Pages 297-308
Organization of the Cation Binding Domain of the Na + /K + -pump....Pages 309-320
The Stabilization of Cation Binding and its Relation to Na + /K + -ATPase Structure and Function....Pages 321-331
Is the Sodium Pump a Functional Dimer?....Pages 332-341
Atomic Force Microscopy of Crystallized Na + /K + -ATPase....Pages 342-345
Scanning force microscopical studies of the Na + /K + -ATPase....Pages 346-349
Molecular Imaging of Purified Na + /K + -ATPase Molecules in Canine Kidney Membranes Using Atomic Force Microscopy....Pages 350-353
Cytoplasmic and Extracellular Domains in Two-dimensional Crystals of Na + /K + -ATPase....Pages 354-357
Membrane Topology of the α Subunit of Na,K-ATPase....Pages 358-361
Identification of epitopes recognized by Na + /K + -ATPase-directed antibodies....Pages 362-365
Probing the α-subunit folding by dot-sandwich immunochemical analysis....Pages 366-369
Determination of sidedness of COOH-terminus of Na + /K + ATPase alpha subunit by vectorial labeling....Pages 370-373
Approaches to Disulfide Bonds Identification in the Na + /K + -ATPase Alpha Subunit....Pages 374-376
Is the Cysteine 104 Part of the Sugar Binding Subsite of the Cardiac Glycoside Receptor of Na + /K + -ATPase?....Pages 377-380
Towards Understanding the Molecular Mechanism of the Inhibitory Cardiac Glycoside Interaction with the Receptor Enzyme Na + /K + -Transporting ATPase....Pages 381-384
Palytoxin induces K + release from yeast cells expressing Na + , K + -ATPase.....Pages 385-388
Bafilomycin and Concanamycin Derivatives as Specific Inhibitors of P- and V-Type ATPases....Pages 389-392
Tertiary Amines as Cytosolic K + -Antagonists of Gastric H + /K + -ATPase....Pages 393-396
Probing the cation binding site of Na + /K + -ATPase with competitive Na + antagonists....Pages 397-400
Structure of the Cation Occlusion Domain of Na + /K + ATPase Studied by Proteolysis, Interaction with Ca 2+ ions and Thermal Denaturation....Pages 401-404
Functional consequences of alterations to Glu329 and other residues in the transmembrane sector of Na + /K + -ATPase....Pages 405-408
Identification of Glu 779 as an Essential Part of the Cation Binding Site of the Sodium Pump....Pages 409-412
Rearrangements of the membrane-associated segments of Na + /K + -ATPase upon phosphorylation, ion-occlusion and ouabain binding....Pages 413-416
Na + /K + -ATPase: Regulation of K + Access Channels by K + ....Pages 417-420
The Ribose Conformation of ATP Affects the Kinetic Properties of Na + /K + -ATPase....Pages 421-424
ATP accelerates phosphorylation of the Na + /K + -ATPase acting with low apparent affinity....Pages 425-428
Effects of ATP on the Steady-State Level of the Phosphoenzyme of Pig Kidney Na + /K + -ATPase....Pages 429-432
A Phase-Change Model of Transient Kinetics of Phosphoenzyme of Na + /K + -ATPase....Pages 433-436
The E 2 Conformation of Na + /K + -ATPase During the Na + ATPase Activity....Pages 437-440
Parallel Pathway Models for Electric Organ Na + - and Na + /K + -ATPases....Pages 441-444
Enzymic activities of solubilised and dissociated (α + ß) chains and of soluble αß protomers of pig kidney Na + /K + -ATPase....Pages 445-448
One Phosphorylation Site per αβ-Protomer in Reconstituted Shark Na + /K + -ATPase.....Pages 449-452
Protomer Association of Solubilized Na + /K + -ATPase Induced by ATP....Pages 453-456
Arginine Appears not to be Located in the Active Site of Na + /K + -ATPase....Pages 457-459
Does The α-Subunit of the Red Cell Membrane Na + /K + -ATPase Exist as a Homodimer?....Pages 460-463
Functional Diversity of Tissue-Specific Na + /K + -Pumps Delivered from Exogenous Sources into Erythrocytes....Pages 464-467
Two-sided bi-directional Na + /K + - ATPase-liposomes. Structure and function....Pages 468-471
Voltage Sensitivity of the Na + /K + Pump: Structural Implications....Pages 472-481
Electrogenic Properties of the Endogenous and of Modified Torpedo Na + /K + -Pumps in Xenopus Oocytes: The Access Channel for External Cations....Pages 482-494
Electrogenic and electroneutral partial reactions in Na + /K + ATPase from eel electric organ....Pages 495-506
Flexibility and Constraint in the Interpretation of Na + /K + Pump Electrogenicity: What is an Access Channel?....Pages 507-516
Partial Reactions in Na + /K + - and H + /K + -ATPase Studied with Voltage-sensitive Fluorescent Dyes....Pages 517-528
Changes of membrane capacitance coupled with electrogenic transport by the Na + /K + -ATPase....Pages 529-532
Reversal potential of Na + pump current in cardioballs at various ΔG of ATP hydrolysis....Pages 533-536
Characteristics of sodium pump current in guinea-pig and rat dorsal root ganglion neurons....Pages 537-540
Kinetic Analysis and Voltage Dependency of Alpha-3 Na + /K + -Pump....Pages 541-544
Access Channel Model for Na + and K + Translocation by the Na + /K + -pump....Pages 545-548
Na + /K + -pump mutant with a slow charge translocating step....Pages 549-552
Analysing the Na + /K + -pump in Outside-out Giant Membrane Patches of Xenopus Oocytes....Pages 553-556
The Role of Putative Intramembraneous Glutamic Acid Residues of the α-Subunit of the Sodium Pump in External Cation Binding....Pages 557-560
Conformational States of Na + /K + -ATPase Probed with RH-421 and Eosin....Pages 561-564
Do Lipophilic Anions Affect the Spatial Organization of the ATP Binding Site of the Na + /K + -ATPase?....Pages 565-568
Na + /K + -ATPase: Probing for a High Affinity Binding Site for Na + by Spectrofluorometry....Pages 569-572
Interaction between cytoplasmic sodium binding sites and cardiotonic steroids....Pages 573-576
Dielectric Coefficients of the Extracellular Release of Sodium Ions....Pages 577-580
Changes in the Conformational State of Probe-Labeled Na + /K + -ATPase in Real Time....Pages 581-592
Mechanism of the Conformational Change in Fluorescein 5′-Isothiocyanate-Modified Na + /K + ATPase....Pages 593-604
Relaxation Spectroscopy Applied to Determination of Rate Constants for Ligand Binding and Dissociation....Pages 605-608
Does Eosin Treat all P-type ATPases Equally?....Pages 609-612
Kinetic Analyses of Conformational Changes and Charge Transfer by Na + /K + -ATPase....Pages 613-616
Reassignment of cation-induced population of main conformational states of FITC-Na + /K + -ATPase as detected by fluorescence spectroscopy and characterized by equilibrium binding studies....Pages 617-620
Caged Na + and K + ligands: photochemical properties, application for membrane transport studies and selective fluorimetric detection of alkali ions....Pages 621-624
Kinetic and spectroscopic characterisation of the cardiac glycoside binding site of Na + /K + -ATPase employing a dansylated ouabain derivative....Pages 625-628
Kinetics and mechanism of cation-binding to Na + ,K + -ATPase....Pages 629-632
Comparison of E 1 and E 2 Conformers of Membrane Bound Na + /K + -ATPase Using Time-Resolved Phosphorescence Anisotropy Measurements....Pages 633-636
Application of Time-Resolved Phosphorescence Anisotropy Measurement for the Study of Rotational Mobility of Eosin-Labelled Na + /K + -ATPase and Ca 2+ ATPase....Pages 637-640
Pyridoxal-5’ -phosphate Probe at Lys 480 can Monitor Conformational Events Induced by Acetyl Phosphate in Na + /K + -ATPase....Pages 641-644
Phosphorylation of Na + /K + -ATPase by p-Nitrophenylphosphate and Other Phosphatase Substrates....Pages 645-648
Estimation of Distance Changes Between Cys-964 and Lys-501 in Na + /K + -ATPase Intermediates and the Orientation of Transmembrane Segment Containing Cys-964....Pages 649-652
Heterogeneity of distribution and of regulation by corticosteroids of transport ATPases along the rat collecting tubule....Pages 653-661
Hormonal regulation of Na + ,K + -ATPase activity....Pages 662-669
Regulation of the Na + ,K + -pump by Insulin....Pages 670-681
Phosphorylation of Na,K-ATPase by Protein Kinases: Structure-Functions Relationship....Pages 682-685
Stimulation by Phorbol 12-Myristate 13-Acetate (PMA) of Na,K-Pump Mediated 86 Rb + -Influx Into Human Lymphocytes....Pages 686-689
Regulation of the Sodium Pump by Fatty Acids and Acylglycerols....Pages 690-693
Relations of Na + /K + -Pump Kinetics to Polyunsaturated Molecular Species of Plasmalogen-Phospatidylethanolamine in Intact Human Erythrocytes....Pages 694-697
Inhibitory Actions of Monoclonal Antibody to Phosphatidylserine on Na + /K + -ATPase....Pages 698-701
Regulation of sodium pump with physiologically active ligands (prostaglandins, galanin)....Pages 702-703
Characterization of the molecular isoforms of Na + /K + -ATPase submits along the rat nephron by polymerase chain reaction....Pages 704-709
Increase in Na Pump Activity of Brain-type Isoforms via Increased Turnover Rate after Glutamate Excitation of Cerebral Neurons....Pages 710-713
The α3 isoform protein of the Na + /K + -ATPase is associated with the sites of neuromuscular and cardiac impulse transmission....Pages 714-717
Direct Correlation of Na + /K + -ATPase -Isoform Abundance and Myocardial Contractility in Mouse Heart....Pages 718-721
Discovery of Endogenous Ouabain — A New Mammalian Hormone....Pages 722-731
Structure and Biological Activity of the Na + /K + -ATPase Inhibitor Isolated from Bovine Hypothalamus: Difference from Ouabain....Pages 732-742
Identification and physiological significance of Na + /K + -pump inhibitors in rats....Pages 743-754
Endogenous Digitalis-Like Factor from Umbilical Cord and Ouabain: Comparison of Biochemical Properties....Pages 755-758
New steroidal digitalis-like compounds in human cataractous lenses....Pages 759-762
Large Scale Purification of an Endogenous Na + /K + -pump Inhibitor from Bovine Adrenal Glands....Pages 763-766
Demonstration of Inhibitors of the Sodium Pump in Human Plasma and Bovine Adrenals Cross-Reacting with Proscillaridin A Antibodies....Pages 767-770
A Labile Endogenous Na-pump Inhibitor in Man....Pages 771-774
Ouabain-like immunoreactivity in the brain and the adrenal medulla is increased in DOCA-salt hypertensive rats....Pages 775-778
Changes in the Endogenous Ouabain-like Substance in the Brain and Cerebrospinal Fluid after Cold-induced Vasogenic Brain Edema....Pages 779-782
Modulation of the Ca 2+ /Mg 2+ -ATPase Activity of Synaptosomal Plasma Membrane Vesicles by the Hypothalamic Hypophysary Na + /K + -ATPase Inhibitory Factor (HHIF)....Pages 783-786
Endogenous Digitalis in Acute Myocardial Ischemia....Pages 787-790
Sensitivity of the failing and nonfailing human heart to cardiac glycosides....Pages 791-796
Autoimmune gastritis and H+/K+-ATPase....Pages 797-806
Na + Modulation of K + Transport by Na + /K + -Pump in Red Cells of Salt-sensitive and Salt-resistant Dahl Rats....Pages 807-811
Biochemical characterization of the Na + /K + -ATPase isoforms in human heart....Pages 812-815
The Canine Cardiac α 3 Isoform of Na + /K + -ATPase is Highly Sensitive to Digitoxigenin....Pages 816-819
Heterogeneous Na + Sensitivities of Na + /K + -ATPase Isoenzymes in Membranes ; Modulation by Anaesthesia and Diets....Pages 820-823
Na + /K + -ATPase Isoforms in Human Heart; Variation with Mammalian Species and Pathophysiological States....Pages 824-827
Non Effectiveness of Ouabain and Decrease in Na + /K + -ATPase Affinity for Ouabain in Failing Rabbit Heart....Pages 828-831
Digoxin Treatment and Congestive Heart Failure in Light of Human Cardiac and Skeletal Muscle Digitalis Glycoside Receptor Studies....Pages 832-835
Age-Related Changes in Na + /K + -ATPase and Ca 2+ -ATPase Concentration in Rat Heart Ventricle....Pages 836-839
Digoxin Induced Dysfunction of Skeletal Muscle Potassium Homeostasis During Exercise....Pages 840-843
Na + /K + -ATPase Isoform Expression in Cardiac Muscle from Dahl Salt-Sensitive and Salt-Resistant Rats....Pages 844-847
Na + /K + -ATPase from spontaneously hypertensive rats....Pages 848-851
Na + /K + -pump α3 Isoform in Rat Vascular Smooth Muscle and its Physiological Role....Pages 852-855
Hormonal Effects on the Expression of Na + /K + -ATPase Beta Subunit During Pregnancy....Pages 856-859
Does Human Skeletal Muscle Na + /K + -ATPase Concentration Correlate With Capacity for Muscle Performance?....Pages 860-863
Regulation of the Na + /K + -pump proteins in skeletal muscle: Acute effects of insulin on α2 distribution and muscle fibre-type specific expression of β subunits....Pages 864-867
Insulin as Regulator of Skeletal Muscle Na + /K + -ATPase Concentration in Rats With Experimental Diabetes and Patients With Diabetes Mellitus....Pages 868-871
The maximum capacity for active sodium-potassium transport in the slow and fast skeletal muscle of obese (ob/ob) and lean mice....Pages 872-875
Alterations in Na + /K + ATPase Isoforms of Different Tissues in Streptozotocin Induced Diabetes Mellitus....Pages 876-879
Properties of the Oxidized Na + /K + -ATPase....Pages 880-883
Modification of Na + /K + -ATPase in a Model of Epilepsy in the Rat....Pages 884-887
Tumor development and changes in Na + /K + -pump parameters....Pages 888-890
Distinct Interaction of Mercury and Silver with Isolated Na + /K + -ATPase....Pages 891-894
Back Matter....Pages 895-906