The Maize Handbook

Front Matter....Pages i-xxvi
Front Matter....Pages 1-1
Fertilization and Embryogeny in Maize....Pages 3-10
The Maize Shoot....Pages 11-17
The Maize Leaf....Pages 17-28
The Maize Root....Pages 29-37
Morphology and Development of the Tassel and Ear....Pages 37-47
Gametogenesis in Maize....Pages 48-61
The Patterns of Plant Structures in Maize....Pages 61-65
Genetics and the Morphological Evolution of Maize....Pages 66-77
Overview of Key Steps in Aleurone Development....Pages 78-80
Front Matter....Pages 81-81
Light Microscopy I: Dissection and Microtechnique....Pages 83-95
Light Microscopy II: Observation, Photomicrography, and Image Analysis....Pages 95-108
Scanning Electron Microscopy....Pages 108-117
Transmission Electron Microscopy: Chemical Fixation, Freezing Methods, and Inmunolocalization....Pages 118-134
Techniques for Histology of Maize Megaspores and Embryo Sacs....Pages 135-139
Indirect Immunofluoreseence: Localization of the Cytoskeleton....Pages 140-149
Immunocytochemistry for Light and Electron Microscopy....Pages 149-157
Immunolocalization of Nuclear Proteins....Pages 158-164
In situ Hybridization....Pages 165-180
Photography of Maize....Pages 180-185
Front Matter....Pages 187-187
Genetic Experiments and Mapping....Pages 189-197
Front Matter....Pages 187-187
Growing Maize for Genetic Studies....Pages 197-209
A Nine-Step Way to Characterize a Morphological Mutant....Pages 209-211
Mutagenesis....Pages 212-219
Gene Tagging with Ac/Ds Elements in Maize....Pages 219-233
Using Cytogenetics to Enhance Transposon Tagging with Ac Throughout the Maize Genome....Pages 234-239
Transposon Tagging with Spm....Pages 240-242
Transposon Tagging with Mutator....Pages 243-249
Mapping Genes with Recombinant Inbreds....Pages 249-254
The Placement of Genes Using waxy-Marked Reciprocal Translocations....Pages 255-257
Chimeras for Genetic Analysis....Pages 258-262
The Use of Clonal Sectors for Lineage and Mutant Analysis....Pages 262-270
Use of Segmental Aneuploids for Mutant Analysis....Pages 270-273
Biased Transmission of Genes and Chromosomes....Pages 274-279
Anthocyanin Genetics....Pages 279-281
Cloned Anthocyanin Genes and Their Regulation....Pages 282-285
 Maize and Puccinia sorghi: A System for the Study of the Genetic and Molecular Basis of Host-Pathogen Interactions ....Pages 286-290
Disease Lesion Mutants....Pages 291-296
Classification of Pollen Abortion in the Field....Pages 297-298
Genetic Fine Structure as Revealed in Pollen Assays....Pages 298-302
Genetic Fine Structure from Testcross Progeny Analysis....Pages 303-306
Front Matter....Pages 187-187
Trisomic Manipulation....Pages 307-308
B-A Translocation Manipulation....Pages 308-314
Locating Recessive Genes to Chromosome Arm with B-A Translocations....Pages 315-327
Dosage Analysis Using B-A Translocations....Pages 328-329
Marker Systems for B-A Translocations....Pages 330-332
Construction of Compound B-A Translocations....Pages 332-334
A-B-A Compound Chromosomes....Pages 334-335
Comprehensive List of B-A Translocations in Maize....Pages 336-341
Chromosomal Translocations Involving the Nucleolus Organizer Region or Satellite of Chromosome 6....Pages 342-345
Inversions and List of Inversions Available....Pages 346-349
Use of Maize Monosomics for Gene Localization and Dosage Studies....Pages 350-358
Marker Systems for r-x1....Pages 359-360
Translocations as Genetic Markers....Pages 361-363
A-A Translocations: Breakpoints and Stocks....Pages 364-376
Segmental Aneuploid Analysis....Pages 377-383
Directed Synthesis of Segmental Transpositions....Pages 383-385
Practical Aspects of Haploid Production....Pages 386-388
Indeterminate Gametophyte ( ig ): Biology and Use....Pages 388-393
Production of a Ploidy Series....Pages 394-395
Absorption Cytophotometry of Nuclear DNA....Pages 396-400
Front Matter....Pages 187-187
Flow Cytometry for Endosperm Nuclear DNA....Pages 400-406
Allelism Testing of Lethal Mutations....Pages 407-413
Analysis of Cytoplasmically Inherited Mutants....Pages 413-417
Male Sterility and Restorer Genes in Maize....Pages 418-423
Inbred Lines of Maize and Their Molecular Markers....Pages 423-432
Traditional Analysis of Maize Pachytene Chromosomes....Pages 432-441
Techniques for Preparing Whole-Mount Spreads of Maize Pachytene Chromosome Complements for Electron-Microscopic Visualization of Synaptonemal Complex Structures....Pages 442-446
A Smear Technique for the Study of Meiosis in Pollen Mother Cells of Maize....Pages 447-449
Protocol for Preparing Maize Macrospore Mother Cells for the Study of Female Meiosis and Embryo-sac Development....Pages 450-454
Preparing a Suspension of Microsporocytes for Spreading and Electron Microscopy....Pages 454-456
Three-dimensional Fluorescence Microscopy of Maize Chromosomes....Pages 457-459
Chromosomal Behavior During Microsporogenesis....Pages 460-475
A Staining Procedure for Pollen Grain Chromosomes of Maize....Pages 476-480
A Technique for the Preparation of Somatic Chromosomes of Maize....Pages 481-483
A Technique for Somatic Chromosome Preparation and C-banding of Maize....Pages 484-493
Duplications....Pages 493-494
Deficiency Analysis....Pages 494-495
The Gametophyte Factors of Maize....Pages 496-503
Ring Chromosomes....Pages 503-504
In situ Hybridization of DNA and RNA Probes to Maize Chromosomes....Pages 504-508
Front Matter....Pages 187-187
Analysis of Traits With Complex Inheritance in Maize Using Molecular Markers....Pages 509-513
Heterofertilization....Pages 514-515
Front Matter....Pages 517-517
Isolation of Small Nuclear RNAs....Pages 519-521
Plant DNA Miniprep and Microprep: Versions 2.1–2.3....Pages 522-525
Urea-based Plant DNA Miniprep....Pages 526-527
High-Molecular-Weight Plant DNA Preparation for CHEF Gel Analysis....Pages 528-529
Preparation of High-Molecular-Weight Maize DNA and Analysis by Pulsed-Field Gel Electrophoresis....Pages 530-533
Isolation of Genomic DNA from Calli....Pages 534-535
Isolation of DNA from Immature Cobs....Pages 536-537
Preparation of Nucleic Acids from Maize Microspores and Pollen....Pages 538-540
Preparation of DNA and RNA from Leaves: Expanded Blades and Separated Bundle Sheath and Mesophyll Cells....Pages 541-545
Isolation of RNA from Wx and wx Endosperms....Pages 545-546
RNA Isolation From Electroporated Protoplasts....Pages 547-548
Procedures for Isolating Mitochondria and Mitochondrial DNA and RNA....Pages 549-556
Isolation of Maize Chloroplasts and Chloroplast DNA....Pages 556-558
In vitro Capping of Maize Mitochondrial RNA and Transcription Initiation Site Characterization by RNase Protection....Pages 559-565
Editors’ Note....Pages 565-565
Southern Blots of Maize Genomic DNA....Pages 566-568
Southern Blot Hybridization....Pages 569-572
Northern Blotting....Pages 572-574
Front Matter....Pages 517-517
RNase Protection Assay....Pages 575-578
Genomic Sequencing in Maize....Pages 579-585
The Polymerase Chain Reaction: Applications to Maize Transposable Elements....Pages 586-592
In vitro Synthesis of Capped and Polyadenylated mRNA for Translational Studies in vitro and in vivo....Pages 592-595
Construction of a Genomic Library in Lambda Phage....Pages 595-598
Agroinfection....Pages 599-603
Polyethylene Glycol-mediated DNA Uptake into Maize Protoplasts....Pages 603-609
DNA Delivery into Maize Cell Cultures Using Silicon Carbide Fibers....Pages 610-613
Transient Gene Expression Assay by Electroporation of Maize Protoplasts....Pages 613-615
Assay of Bacterial Chloramphenicol Acetyl Transferase in Transformed Maize Tissues....Pages 615-618
Assay of Firefly Luciferase in Transformed Maize Tissues....Pages 619-621
Storage of Frozen Maize Tissue....Pages 622-623
Maize Methods—Starch Biosynthetic Genes....Pages 624-629
Identifying and Characterizing the TATA Box Promoter Sequence Element in a Maize Nuclear Gene....Pages 630-633
Promoters....Pages 633-636
Introns....Pages 636-639
Characterization of Zein Genes and Their Regulation in Maize Endosperm....Pages 639-647
Overview of Cloning Genes Using Transposon Tagging....Pages 647-652
How RFLP Loci Can Be Used to Assist Transposon-Tagging Efforts....Pages 653-659
Front Matter....Pages 661-661
Regeneration of Plants from Somatic Cell Cultures: Applications for in vitro Genetic Manipulation....Pages 663-671
Front Matter....Pages 661-661
Initiation, Maintenance, and Plant Regeneration of Type II Callus and Suspension Cells....Pages 671-677
Production of Transgenic Maize Plants via Microprojectile-Mediated Gene Transfer....Pages 677-684
In vitro Selection....Pages 685-689
Selection of Stable Transformants From Black Mexican Sweet Maize Suspension Cultures....Pages 690-694
Maize Protoplast Culture....Pages 695-700
Anther and Microspore Culture....Pages 701-704
In vitro Culture of Maize Kernels....Pages 705-708
In vitro Ear Culture System....Pages 709-712
Maize Inflorescence Culture....Pages 712-714
Shoot Meristem Culture....Pages 715-719
Establishment and Culture of Maize Endosperm....Pages 719-722
In vitro Pollen Germination....Pages 723-724
Axillary Bud in vitro Culture: Asexual Propagation of Maize....Pages 725-726
Back Matter....Pages 727-761