The Cytoskeleton Part A. Cytoskeletal Proteins, Isolation and Characterization

Content: 
Advisory Board
Page ii

Edited By
Page III

Copyright Page
Page iv

Contributors
Pages xiii-xiv

Preface
Page xv
Leslie Wilson

Chapter 1 The Cytoskeleton: A Perspective Original Research Article
Pages 1-8
B.R. Brinkley

Chapter 2 Purification and Chemical Properties of Brain Tubulin Original Research Article
Pages 9-30
James C. Lee

Chapter 3 Assembly-Disassembly Purification and Characterization of Microtubule Protein without Glycerol Original Research Article
Pages 31-49
Douglas B. Murphy

Chapter 4 A Brain Microtubule Protein Preparation Depleted of Mitochondrial and Synaptosomal Components Original Research Article
Pages 51-60
Timothy L. Karr, Hillary D. White, Beth A. Coughlin, Daniel L. Purich

Chapter 5 Purification and Reassembly of Tubulin from Outer Doublet Microtubules Original Research Article
Pages 61-78
Kevin W. Farrell

Chapter 6 Production of Antisera and Radioimmunoassays for Tubulin Original Research Article
Pages 79-96
Livingston Van De Water III, Susan D. Guttman, Martin A. Gorovsky, J.B. Olmsted

Chapter 7 Immunofluorescence and Immunocytochemical Procedures with Affinity Purified Antibodies: Tubulin-Containing Structures Original Research Article
Pages 97-132
Mary Osborn, Klaus Weber

Chapter 8 An Automated Method for Defining Microtubule Length Distributions Original Research Article
Pages 133-144
David Kristofferson, Timothy L. Karr, Thomas R. Malefyt, Daniel L. Purich

Chapter 9 Measurement of Steady-State Tubulin Flux Original Research Article
Pages 145-158
Robert L. Margolis

Chapter 10 A Rapid Filtration Assay for Analysis of Microtubule Assembly, Disassembly, and Steady-State Tubulin Flux Original Research Article
Pages 159-169
Leslie Wilson, K. Bradford Snyder, William C. Thompson, Robert L Margolis

Chapter 11 A Direct Method for Analyzing the Polymerization Kinetics at the Two Ends of a Microtubule Original Research Article
Pages 171-187
Gary G. Borisy, Lawrence G. Bergen

Chapter 12 Dynein Decoration of Microtubules–Determination of Polarity Original Research Article
Pages 189-206
Leah T. Haimo

Chapter 13 Microtubule Polarity Determination Based on Conditions for Tubulin Assembly In Vitro Original Research Article
Pages 207-216
Steven R. Heidemann, Ursula Euteneuer

Chapter 14 The Use of Tannic Acid in Microtubule Research Original Research Article
Pages 217-233
Keigi Fujiwara, Richard W. Linck

Chapter 15 The Cyclic Tyrosination/Detyrosination of Alpha Tubulin Original Research Article
Pages 235-255
William C. Thompson

Chapter 16 Tubulin-Tyrosine Ligase from Brain Original Research Article
Pages 257-263
Martin Flavin, Takaaki Kobayashi, Todd M. Martensen

Chapter 17 Preparation of Brain Tyrosinotubulin Carboxypeptidase Original Research Article
Pages 265-269
Todd M. Martensen

Chapter 18 Purification of Muscle Actin Original Research Article
Pages 271-289
Joel D. Pardee, James A. Spudich

Chapter 19 Directional Growth of Actin Off Polylysine-Coated Beads Original Research Article
Pages 291-300
Susan S. Brown

Chapter 20 A Falling Ball Apparatus to Measure Filament Cross-linking Original Research Article
Pages 301-311
Thomas D. Pollard

Chapter 21 Antibody Production and Immunofluorescent Characterization of Actin and Contractile Proteins Original Research Article
Pages 313-331
Elias Lazarides

Chapter 22 Myosin Purification and Characterization Original Research Article
Pages 333-371
Thomas D. Pollard

Chapter 23 Preparation and Purification of Dynein Original Research Article
Pages 373-397
Christopher W. Bell, Clarence Fraser, Winfield S. Sale, Wen-Jing Y. Tang, I.R. Gibbons

Chapter 24 Isolation and Characterization of Intermediate Filaments Original Research Article
Pages 399-419
Peter Steinert, Robert Zackroff, Martha Aynardi-Whitman, Robert D. Goldman

Index
Pages 421-427

Contents of Recent Volumes
Pages 429-445