The Art and Science of Assisted Reproductive Techniques

Book Cover......Page 1
Half-Title......Page 2
Title......Page 4
Copyright......Page 5
Dedications......Page 6
Foreword......Page 8
Preface......Page 11
List of Contributors......Page 14
Contents......Page 28
Section 1: Introduction......Page 35
1. Advent of Medically Assisted Reproductive Technologies (MART) in India......Page 37
2. The Endocrinology of ART......Page 42
3. Efficient Classification of Infertility......Page 56
4. Modern Work-up of Infertility......Page 67
Section 2: Stimulation Strategies......Page 75
5. Ovulation Induction with Tamoxifen Citrate......Page 76
6. Defining the Poor Ovarian Response before Controlled Ovarian Hyperstimulation......Page 85
7. Aromatase InhibitorsTheir Role in the Treatment of Infertility......Page 105
8. Urinary Human FSH Versus Recombinant Human FSH......Page 110
9. Stimulation Strategies for Complex IVF Patients......Page 117
10. Programming the Cycle with Oral Contraceptives Antecedent to the use of Antagonists......Page 129
11. Agonists Versus Antagonists: Physiology to Clinical Success......Page 135
12. Microdose GnRH for the Stimulation of Low Responders......Page 146
13. The Role of GnRH Antagonist in the Management of Poor Responders......Page 152
14. Alternative Approaches to Ovarian Stimulation and Triggering of Ovulation......Page 158
15. Role of LH in Stimulation Protoco Is for ART......Page 194
16. Role of hMG-HP in Stimulated Cycles for ART......Page 204
17. Luteal Phase Support......Page 219
18. ’Severe OHSS: A Critical Care Physicians Point of View......Page 230
19. Ovulation Induction: Surgical Approach......Page 235
Section 3:  POLYCYSTIC OVARY SYNDROME (PCOS)......Page 254
20. Polycystic Oυary Syndrome (PCOS): An Update......Page 255
21. In vitro Oocyte Maturation......Page 268
22. Polycystic Ovary Syndrome: Genetics and Health Consequences......Page 271
Section 4: ART Procedures......Page 284
23. Vaginal Oocyte Retrieυal......Page 285
24. Gamete Intrafallopian Transfer (GIFT)......Page 301
25. Zygote Intrafallopian Tube Transfer (ZIFT): Patient Selection is the Key to Beneficial Utilization......Page 311
26. Fallopian Tube Sperm Perfusion......Page 317
27. Use of Lasers in ART: Clinical Applications and Potential Research Tools......Page 326
28. Blastocyst Transfer: One Step Further in the Quest for the Magic Bullet......Page 340
Section 5: Laboratory Issues......Page 347
29. Semen Analysis for Clinical Interpretation......Page 348
30. Fundamentals of Sperm Processing Techniques......Page 361
31. Sperm Separation Techniques: Comparison and Evaluation of Gradient Products......Page 369
32. Prediction of ART Outcome in Male Factor Infertility Patients by a New Semen Quality Score......Page 375
33. Why Should We Assess Oocyte and Embryo Morphology?......Page 398
34. Benefits and Drawbacks of Extended Embryo Culture......Page 407
35. Really, Just How Important is the Level of Room Lighting in the IVF Laboratory on Embryo Development?......Page 414
36. The Mouse Embryo Bioassay: Is It the “Gold Standard” for Quality Control Testing in the IVF Laboratory?......Page 421
37. Human Oocyte and Embryo Cryopreservation......Page 427
38. In Vitro Maturation: Future Clinical Applications......Page 441
39. Oxidative Stress and DNA Damage in Human Sperm: The Cleveland Clinic Story......Page 453
40. Quality Management in an Assisted Reproductive Therapy Environment......Page 480
Section 6: Contemporary Thoughts......Page 487
41. How to Improve Success Rates in IVF?......Page 488
42. Current Immunological Assays: Are they Enough to Uncover the Supposed Immune Causes for Assisted Reproduction Failure?......Page 495
43. Endometriosis and ART......Page 500
44. Infertility: Is ihere Success afterForty?......Page 506
45. Inheritance of Infertility......Page 515
46. Sperm Sepamtion......Page 525
47. Ovarian Tissue Cryopreservation in Cancerous Patients: State of the Art......Page 537
48. Molecular Biology Applied to ART......Page 558
49. Assisted Reproductive Technologies in Human Immunodeficiency Virus (HIV) Sero-discordant Couples: Practice, Prognosis and Future Prospects......Page 567
Section 7: Third Party Reproduction......Page 574
50. Gestational Surrogacy......Page 575
51. Oocyte Donation......Page 587
52. Oocyte-Sharing Programs......Page 595
53. Germinal Stem Cells: Culture and Replication......Page 605
54. Cytoplasmic and Nuclear Transfer: New Life for an Old Egg?......Page 621
Section 8: Implantation......Page 631
55. The Endometrium and Implantation......Page 632
56. Modulators of Endometrial Receptivity: A Molecular Symphony......Page 643
57. Endometrial Preparation for Patients Undergoing Frozen-Thawed Embryo Transfer Cycles......Page 657
58. Pathophysiology of Implantation Failure in IVF......Page 663
59. Recurrent Implantation Failures: The Preferred Therapeutic Approach......Page 676
60. Repeated Pregnancy Loss (RPL): Is Investigation Important?......Page 688
61. Antiphospho lipid Antibodies in ART......Page 693
Section 9: Cryopreservation......Page 707
62. Cryopreservation of Oocytes and Embryos......Page 708
63. Cryopreservation of Human Spermatozoa......Page 718
Section 10: Endoscopy and ART......Page 727
64. Fertility Following Laparoscopic Surgery and Hysteroscopic Surgery......Page 728
65. The Role of Hysteroscopy in the Management of Infertility......Page 744
66. Modern Management of the Ischemic Black-Blue Twisted Adenexa......Page 762
67. Fertiloscopy: A New Technique and an Alternative to Conventional Laparoscopy in Infertility......Page 768
68. Hysteroscopic Assessment of Selective Tubal Pressures and Tubal Cannulation by Air Bubble Stents......Page 783
69. Intrauterine Adhesions......Page 787
Section 11: Ultrasonography......Page 798
70. Update on Ultrasound Guided Embryo Transfer......Page 799
71. Cervical Mucus Evaluation by Transvaginal Ultrasonography: A Novel Approach......Page 811
72. Multifetal Pregnancy Reduction......Page 819
Section 12: The Male Factor......Page 825
73. Non-obstructive Azoospermia: Predictive Criteria for Sperm Retrieval......Page 826
74. Epididymal and Testicular Sperm Retrieval......Page 832
75. Microdeletions in the Y-Chromosome and Male Infertility......Page 840
76. Isolated Teratozoospermia—ICSI or IUI......Page 846
77. Hormone Substitution in Male Infertility......Page 855
Section 13:  Preimplantation Genetic Diagnosis (Pgd)......Page 860
78. Preimplantation Genetic Diagnosis in Cases with Abnormal Gamete Cell Morphology......Page 861
79. Preimplantation Genetic Diagnosis (PGD) for Sex Selection......Page 872
Section 14:  Present And Future Of Infertility......Page 877
80. Present and Future of Infertility Therapy......Page 878
81. Patient Support in the ART Program......Page 890
82. Politics, Partnerships and IVF......Page 905
83. ’IVF Success from a Clinicians Viewpoint: How to Get it, How to Keep it?......Page 915
84. Bandwagon IVF: All for One and One for All......Page 926
Index......Page 938