Techniques in Protein Chemistry IV

Content: 
Front Matter, Page iii
Inside Front Cover, Page ifc1
Copyright, Page iv
Foreword, Page xiii, Mark Hermodson
Preface, Page xv, Ruth Hogue Angeletti
Acknowledgements, Page xvii
Mass Spectrometry in Protein Sequence and Structural Investigations, Pages 3-21, A.L. Burlingame
Elucidation of Covalent Modifications and Noncovalent Associations in Proteins by Electrospray Ionization Mass Spectrometry, Pages 23-31, Joseph A. Loo, Rachel R. Ogorzalek Loo, David R. Goodlett, Richard D. Smith, Alfred F. Fuciarelli, David L. Springer, Brian D. Thrall, Charles G. Edmonds
Identification of Conserved Protein Surface Metal-Binding Sites in Related Proteins by Mass Spectrometry, Pages 33-40, T. William Hutchens, Tai-Tung Yip, Randall W. Nelson
Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of Membrane Proteins: The Scrapie Prion Protein, Pages 41-45, Michael A. Baldwin, Ken-Ming Pan, Rolf Hecker, Neil Stahl, Stanley B Prusiner, Rong Wang, Brian T. Chait
Characterization of the Proteins c-kit Ligand and DHFR by Electrospray Mass Spectrometry, Pages 47-54, Hanno Langen, Bernadette Sander, Francis Vilbois, Hans-Werner Lahm
RAPID PURIFICATION, SEPARATION AND IDENTIFICATION OF PROTEINS AND ENZYME DIGESTS USING PACKED CAPILLARY PERFUSION COLUMN LC AND LC/MS, Pages 55-64, D.B. Kassel, M.A. Luther, D.H. Willard, S.P. Fulton, J.-P. Salzmann
Sample Immobilisation Protocols for Matrix Assisted Laser Desorption Mass Spectrometry, Pages 65-72, K.K. Mock, C.W. Sutton, A. Keane, J.S. Cottrell
Assessing the Multimeric States of Proteins by Matrix-Assisted Laser Desorption Mass Spectrometry: Comparison with Other Biochemical Methods, Pages 73-81, Terry B. Farmer, Richard M. Caprioli
N-TERMINAL MODIFICATION OF MALARIAL ANTIGENS FROM E. coli, Pages 83-90, Jelle Lahnstein, Shanny L. Dyer, Neil H. Goss, Mark Duncan, Raymond S. Norton
Mass Spectrometric Characterisation of C-terminal “Ragged Ends” in Peanut Agglutinin, Pages 91-98, Pierre Thibault, David C. Watson, Makoto Yaguchi, N. Martin Young
ACCURATE DETERMINATION OF MOLECULAR MASS FOR PROTEINS ISOLATED FROM POLYACRYLAMIDE GELS, Pages 99-106, T.N. Asquith, R. Takigiku, M.P. Purdon, D.L. Gauggel, T.W. Keough, M.P. Lacey
Carbohydrate Characterisation of a Glycoprotein by Matrix Assisted Laser Desorption Mass Spectrometry, Pages 109-116, C.W. Sutton, A.C. Poole, J.S. Cottrell
Post-translational Modifications of Recombinant Proteins Determined by LC/Electrospray Mass Spectrometry and High Performance Tandem Mass Spectrometry, Pages 117-125, Katalin F. Medzihradszky, Christine A. Settineri, David A. Maltby, A.L. Burlingame
IDENTIFICATION AND CHARACTERIZATION OF GLYCOSYLATION SITES IN CARCINOEMBRYONIC ANTIGEN (CEA) BY MASS SPECTROMETRY, Pages 127-134, Kristine M. Swiderek, Constance S. Pearson, John E. Shively
Monosaccharide and Oligosaccharide Analysis of Recombinant Erythropoietin Electro-transferred onto Polyvinylidene Fluoride Membranes, Pages 135-142, Michael Weitzhandler, Douglas Kadlecek, Nebojsa Avdalovic, R. Reid Townsend
A Practical Approach for Isolation and Characterization of Glycosylation Sites of Glycoproteins Bearing N- and/or O-Linked Carbohydrate Chains, Pages 143-151, Kuo-Liang Hsi, Ling Chen, Pau-Miau Yuan
Location of the Keratan Sulfate Attachment Sites in the Hyaluronate Binding Region of the Proteoglycan, Aggrecan, Pages 153-159, Peter J. Neame, Frank P. Barry
Site Specific Heterogeneity of N-Linked Oligosaccharides on Recombinant Human Erythropoietin, Pages 161-168, Patricia L. Derby, Thomas W. Strickland, Michael F. Rohde
Analysis of Serine, Threonine and Tyrosine Phosphorylation Sites with Mass Spectrometry, Pages 171-178, John W. Crabb, Charles Johnson, Karen West, Janina Buczylko, Krzysztof Palczewski, Jinzhao Hou, Kerstin McKeehan, Mikio Kan, Wallace L. McKeehan, Michael J. Huddleston, Steven A. Carr
The Identification of Phosphorylation Sites in Large Membrane Proteins Following Their Isolation by SDS-PAGE, Pages 179-186, Karen S. De Jongh, Eric I. Rotman, Brian J. Murphy
Modification of Thiophosphorylated Proteins with Extrinsic Probes, Pages 187-192, Kevin C. Facemyer, Mark R. Tibeau, Christine R. Cremo
A Novel Method of Identifying Phosphorylation Sites Using a Thiophosphorylated Peptide and ESI-MS, Pages 193-200, David R. Stover, Kenneth A. Walsh
Protein Phosphorylation: Sequence-Specific Identification of In Vivo Phosphorylation Sites by MALDI-TOF Mass Spectrometry, Pages 201-210, Tai-Tung Yip, T. William Hutchens
Synthetic Phosphopeptide Epitope Mapping with Mass Spectrometric Verification, Pages 211-225, William T. Moore, Wan-Kyng Liu, Shu-Hui Yen, Frederick L. Hall, Richard M. Caprioli
Evaluation of Peptide Synthesis As Practiced in 53 Different Laboratories, Pages 229-238, Gregg B. Fields, Steven A. Carr, Daniel R. Marshak, Alan J. Smith, John T. Stults, Lynn C. Williams, Ken R. Williams, Janis D. Young
SYNTHESIS, PURIFICATION AND INTERACTIONS OF CASEIN SIGNAL PEPTIDES, Pages 239-248, C. Creuzenet, G. Korshunova, H. Naharisoa, H. Nedev, T. Haertlé
The Systematic Development of Peptides Having Potent Antimicrobial Activity Against E. coli Through the Use of Synthetic Peptide Combinatorial Libraries, Pages 249-256, Richard A. Houghten, Kim Thy Dinh, David E. Burcin, Sylvie E. Blondelle
Synthesis of Proteins by Chemical Ligation of Unprotected Peptide Segments: Mirror-Image Enzyme Molecules, D- & L-HIV Protease Analogs, Pages 257-267, R.C. de Lisle Milton, Saskia C.F. Milton, Martina Schnölzer, Stephen B.H. Kent
DIFFICULT SEQUENCES IN THE SYNTHESIS OF G-PROTEIN FRAGMENTS THAT HARBOR A PUTATIVE ADHESION MOTIF, Pages 269-275, Assaf Steinschneider
Cysteine and Tryptophan Amino Acid Analysis of ABRF92-AAA, Pages 279-288, Daniel J. Strydom, Thomas T. Andersen, Izydor Apostol, Jay W. Fox, Raymond J. Paxton, John W. Crabb
Compositional Protein Analysis Using 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate, a Novel Derivatization Reagent, Pages 289-298, Steven A. Cohen, Kathryn DeAntonis, Dennis P. Michaud
Sensitive Analysis of Cystine/Cysteine using 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate (AQC) Derivatives, Pages 299-306, Daniel J. Strydom, Steven A. Cohen
AMINO ACID ANALYSIS AND MULTIPLE METHYLATION OF LYSINE RESIDUES IN THE SURFACE PROTEIN ANTIGEN OF RICKETTSIA PROWAZEKI, Pages 307-314, W.-M. Ching, H. Wang, G.A. Dasch, J. Davis
CHIRALITY DETERMINATIONS OF D- AND L-AMINO ACIDS OF SYNTHETIC AND NATURALLY OCCURRING PEPTIDES, Pages 315-320, Brenda L. Purcell, Muriel S. Doleman
Development of Separation Strategies for Proteins by Capillary Electrophoresis, Pages 323-332, Judith A. Nolan, Richard Palmieri
High Resolution Full-Range (pI = 2.5 to 10.0) Isoelectric Focusing of Proteins and Peptides in Capillary Electrophoresis, Pages 333-341, Shiaw-Min Chen, John E. Wiktorowicz
Purification of Different Biologically Active Forms of Mouse β-Nerve Growth Factor by Immobilized Metal Ion Affinity Chromatography, Pages 343-351, Giuseppe Corona, Steven D. Skaper, Lanfranco Callegaro
Increasing the Antigen Binding Capacity of Immobilized Antibodies, Pages 353-360, Alvin S. Stren, Frank J. Podlaski
MICROPREPARATIVE CAPILLARY ELECTROPHORESIS (MPCE) AND MICROPREPARATIVE HPLC OF PROTEIN DIGESTS, Pages 363-370, James W. Kenny, J.I. Ohms, Alan J. Smith
Peptide Mapping of 2-D Gel Proteins by Capillary HPLC, Pages 371-378, Susan C. Wong, Christopher Grimley, Allan Padua, James H. Bourell, William J. Henzel
Internal Peptide Sequence of Proteins Digested In-Gel after One- or Two- Dimensional Gel Electrophoresis, Pages 379-387, Pascual Ferrara, Jorge Rosenfeld, Jean Claude Guillemot, Joel Capdevielle
IN SITU TRYPTIC DIGESTION OF PROTEINS SEPARATED BY SDS-PAGE: IMPROVED PROCEDURES FOR EXTRACTION OF PEPTIDES PRIOR TO MICROSEQUENCING, Pages 389-397, Tim Tetaz, Ian Smith, Esther Bozas, Ian Walker, Jerry Kanellos
In Situ Proteolytic Digestions Performed on Proteins Bound to the Hewlett-Packard Hydrophobic Sequencing Column, Pages 399-406, William Burkhart
ROUTINE PROTEIN SEQUENCE ANALYSIS BELOW TEN PICOMOLES: ONE SEQUENCING FACILITY\'S APPROACH, Pages 409-418, Donna Atherton, Joseph Fernandez, Michael DeMott, Lori Andrews, Sheenah M. Mische
Successful Peptide Sequencing With Femtomole Level PTH-Analysis: A Commentary, Pages 419-426, Hediye Erdjument-Bromage, Scott Geromanos, Amy Chodera, Paul Tempst
ON-LINE MICROBORE HPLC DETECTION OF FEMTOMOLE QUANTITIES OF PTH-AMINO ACIDS, Pages 427-433, Russell W. Blacher, John H. Wieser
Single Syringe-Pump Solid-Phase Protein Sequencer, Pages 435-441, Mark Stolowitz, Leroy Hood
ANALYSIS OF COMPLEX PROTEIN MIXTURES ON THE HP-G1000A SEQUENCER, Pages 443-452, T.K. Slattery, R.N. Harkins
PROTEIN SEQUENCING OF POST-TRANSLATIONALLY MODIFIED PEPTIDES and PROTEINS: DESIGN, CHARACTERIZATION AND RESULTS OF ABRF-92SEQ, Pages 453-461, Sheenah M. Mische, K. Ümit Yüksel, Liane M. Mende-Mueller, Paul Matsudaira, Dan L. Crimmins, Philip C. Andrews
Carboxy-terminal Protein Sequence Analysis using Carboxypeptidase P and Electrospray Mass Spectrometry, Pages 463-470, Christine E. Smith, Kevin L. Duffin
Protein Ladder Sequencing: A Conceptually Novel Approach To Protein Sequencing Using Cycling Chemical Degradation and One-Step Readout by Matrix-Assisted Laser Desorption Mass Spectrometry, Pages 471-478, Rong Wang, Brian T. Chait, Stephen B.H. Kent
The Use of Molecular Modelling to Delineate B-Cell and T-Cell Epitopes of Human Sperm-Specific LDH-C4, Pages 481-490, Patricia A. O\'Hern, Erwin Goldberg
Hydrophobic Contact Density Distribution Functions, Pages 491-498, Wayne J. Becktel, Lorraine M. Rellick
Construction and Functional Selection of a T4 Lysozyme Gene Library Randomly Mutagenized at Five Specific Sites, Pages 499-507, Enoch Baldwin, Jian Xu, Omid Hajiseyedjavadi
Horse Heart Ferricytochrome c: Anions, Buffers and Stability, Pages 509-516, Yash P. Myer
Measurement of Amide Hydrogen D/H Fractionation Factors in Proteins by NMR Spectroscopy, Pages 517-524, Stewart N. Loh, John L. Markley
A Model for the Molten Globule State of CTF Generated Using Molecular Dynamics, Pages 525-532, Valerie Daggett
Urea and Guanidine-HCl Yield Different Unfolding Free Energies for CheY: Which Denaturant Provides the Most Reliable Free Energy Values?, Pages 533-540, Gregory T. DeKoster, Andrew D. Robertson, Ann M. Stock, Jeffry B. Stock
Learning an Objective Alphabet of Amino Acid Conformations in Protein, Pages 541-548, Robert T. Miller, Richard J. Douthart, A. Keith Dunker
ΔΔG°(WT-mut) for BPTI Hydrophobic Core Mutants Measured by Hydrogen Isotope Exchange, Pages 549-556, Feng Tao, James Fuchs, Clare Woodward
Thermodynamics of Side Chain Internal Rotations – Effects on Protein Structure and Stability, Pages 557-566, Andrew J. Doig, Mark Gardner, Mark S. Searle, Dudley H. Williams
Evaluation of NMR Based Structure Determination of Flexible Peptides: Application to Desmopressin, Pages 569-575, Jianjun Wang, Frank D. Sönnichsen, Robert Boyko, Robert S. Hodges, Brian D. Sykes
Paramagnetic Proton NMR Methods Used in Studying the Hemeprotein Subunit of Escherichia coli Sulfite Reductase, Pages 577-584, Jeffrey Kaufman, Lewis M. Siegel, Leonard D. Spicer
One- and Two-Dimensional 19F-NMR Methods for Proteins and Nucleic Acids, Pages 585-594, Fraydoon Rastinejad, Pam Artz, Ponzy Lu
“Active Site” Dynamics: A Comparison of the Wild Type and Single Tryptophan Variants of E. Coli Thioredoxin, Pages 595-603, Marvin D. Kemple, Kenneth E. Nollet, Peng Yuan, Franklyn G. Prendergast
Proton Nuclear Magnetic Resonance Studies of Non-Covalent Complexes of Yeast Cytochrome c Peroxidase with Cytochromes c, Pages 605-613, Qian Yi, Steve Alam, Yihong Ge, James D. Satterlee, James E. Erman
The Application of Chemical Shift Calculation to Protein Structure Determination by NMR, Pages 615-622, Timothy S. Harvey, Wilfred F. van Gunsteren
Toward the Structure of Mosaic Proteins: Expression, Purification and Structural Analysis of a Pair of Fibronectin Type1 Modules, Pages 623-631, Michael J. Williams, Isabelle Phan, Martín Baron, Iain D. Campbell, Robin T. Applin
Index, Pages 633-640