Techniques in Protein Chemistry

Content: 
Front Matter, Page iii
Copyright, Page iv
Foreword, Page xiii, David Eisenberg
Preface, Pages xv-xvi, Tony E. Hugli
Acknowledgments, Page xvii
Introduction: CHEMICAL MICROSEQUENCING TECHNIQUES, Pages 3-6, John E. Shively, David W. Speicher
1 - STRATEGIES FOR MICROCHARACTERIZATION OF PROTEINS USING DIRECT CHEMISTRY ON SEQUENCER SUPPORTS, Pages 7-16, Jeffrey D. Hulmes, May C. Miedel, Yu-Ching E. Pan
2 - MANUAL GAS-PHASE MICROSEQUENCING OF PEPTIDES AND PROTEINS, Pages 17-23, Mitsuru Haniu, John E. Shively
3 - MICROSEQUENCING WITH PVDF MEMBRANES: EFFICIENT ELECTROBLOTTING, DIRECT PROTEIN ADSORPTION AND SEQUENCER PROGRAM MODIFICATIONS, Pages 24-35, David W. Speicher
4 - RECOVERY OF BIOLOGICAL ACTIVITY, BLOTTING AND SEQUENCE ANALYSIS OF PROTEIN HORMONES AFTER SDS-PAGE, Pages 36-41, Wolfgang H. Fischer, Joan Vaughan, David Karr, Richard McClintock, Joachim Spiess, Jean Rivier, Wylie Vale
5 - DIRECT SEQUENCE ANALYSIS OF RAT LIVER MEMBRANE GLYCOPROTEINS SEPARATED BY 2D-PAGE, Pages 42-50, David C. Parmelee, Timothy Benjamin, Chien-Hua Niu, Snorri S. Thorgeirsson
6 - THE USE OF PHENYLTHIOCARBAMYL AMINO ACID ESTERS FOR SENSITIVITY ENHANCEMENT IN EDMAN DEGRADATION, Pages 51-58, Marcus J. Horn, Sherrell L. Early, Sheila G. Magil
7 - STUDIES ON C-TERMINAL ANALYSIS, Pages 59-66, David H. Hawke, Suzanne M. Meister, Pau-Miau Yuan, Michael W. Hunkapiller, John H. Richards
8 - STUDIES ON THE USE OF SILYL COMPOUNDS FOR PROTEIN CARBOXY-TERMINAL SEQUENCE ANALYSIS, Pages 67-78, Chad G. Miller, Cheng-te Kong, John E. Shively
9 - AN IMPROVED INJECTION SYSTEM FOR ON-LINE LIQUID CHROMATOGRAPHS: APPLICATION TO HIGH SENSITIVITY PHENYLTHIOHYDANTOIN AMINO ACID ANALYSIS, Pages 79-88, GEOFFREY S. BEGG, RICHARD J. SIMPSON
10 - A SYNTHETIC PEPTIDE FOR EVALUATING PROTEIN SEQUENCER AND AMINO ACID ANALYZER PERFORMANCE IN CORE FACILITIES: DESIGN AND RESULTS, Pages 89-101, Ronald L. Niece, Kenneth R. Williams, Cynthia L. Wadsworth, James Elliott, Kathryn L. Stone, Walter J. McMurray, Audree Fowler, Donna Atherton, Rusty Kutny, Alan J. Smith
11 - PROTEIN SEQUENCING SAMPLE ADVISOR: AN EXPERT SYSTEM TO ASSESS SAMPLE SUITABILITY FOR MICROSEQUENCING, Pages 102-107, Joseph L. Modelevsky, Zafar I. Randawa
12 - DETECTION OF PROTEIN STRUCTURAL FEATURES WITH PROFILE ANALYSIS, Pages 108-117, Michael Gribskov, David Eisenberg
Introduction: Peptide and Protein Characterization by Mass Spectrometry, Pages 121-126, A.L. Burlingame
13 - STRUCTURAL ANALYSIS OF PROTEIN CARBOXYL METHYLTRANSFERASE UTILIZING TANDEM MASS SPECTROMETRY, Pages 127-134, William J. Henzel, John T. Stults, Dana W. Aswad
14 - SEQUENCE DETERMINATION OF N-TERMINALLY BLOCKED AND INTERNAL PEPTIDES AT THE PICOMOLE LEVEL BY TANDEM MASS SPECTROMETRY WITH ARRAY DETECTION, Pages 135-151, Bradford W. Gibson, Zhonghua Yu, Beth Gillece-Castro, William Aberth, F.C. Walls, A.L. Burlingame
15 - A NEW STRATEGY FOR MAPPING THE TOPOGRAPHY OF A TRANSMEMBRANE PROTEIN USING MASS SPECTROMETRY, Pages 152-159, Arnold M. Falick, Stephanie F. Mel, Robert M. Stroud, A.L. Burlingame
16 - LASER PHOTODISSOCIATION FOURIER TRANSFORM MASS SPECTROMETRY: NEW METHODOLOGY FOR SEQUENCE ANALYSIS OF OLIGOPEPTIDES AND LOCATION OF DISULFIDE BONDS, Pages 160-167, Patrick R. Griffin, Jeffrey Shabanowitz, John R. Yates III, Nian Zhou Zhu, Donald F. Hunt
17 - SEQUENCE ANALYSIS OF PROTEIN C-TERMINAL PROTEOLYTIC FRAGMENTS, PROTEIN ISOLATED FROM 2D-GELS, AND MURINE CALBINDIN: NEW METHODOLOGY, Pages 168-175, John R. Yates III, Jeffrey Shabanowitz, Patrick R. Griffin, Nian Zhou Zhu, Donald F. Hunt
18 - LOCATION OF DISULFIDE BONDS IN PROTEINS BY PARTIAL ACID HYDROLYSIS AND MASS SPECTROMETRY, Pages 176-185, Yiping Sun, Zhongrui Zhou, David L. Smith
19 - Evaluation of Methods for the Analysis of Disulfide Containing Peptides by Fast Atom Bombardment Mass Spectrometry, Pages 186-194, Henry Rodriguez, Byron Nevins, John Chakel
20 - EXAMINATION OF THE POSTTRANSLATIONAL MODIFICATIONS OF CARCINOEMBRYONIC ANTIGEN, Pages 195-206, Stanley A. Hefta, Raymond J. Paxton, Laura J.F. Hefta, Terry D. Lee, John E. Shively
21 - THE ASSIGNMENT OF PROTON RESONANCES IN 2D NMR SPECTRA OF PROTEINS, Pages 207-222, S. Walter Englander, A. Joshua Wand, Sarah J. Nelson
22 - SOLUTION STRUCTURE OF TGFα BY 2D 1H NMR AND MOLECULAR MODELLING, Pages 223-232, Bradley A. Katz, Marian Seto, Rick Harkins, James C. Jenson, Brian D. Sykes
23 - STUDY OF A PHOSPHORYLATED PROTEIN BY TWO-DIMENSIONAL NMR SPECTROSCOPY, Pages 233-238, Michael G. Wittekind, Rachel E. Klevit, E. Bruce Waygood
24 - ANALYTICAL ASPECTS OF 2D 1H NMR: PURITY VERSUS CONFORMATIONAL HETEROGENEITY IN CALBINDIN D9k, Pages 239-247, Johan Kördel, Sture Forsén, Walter J. Chazin
Introduction: AMINO ACID ANALYSIS TECHNIQUES, Pages 251-254, John A. Smith
25 - AN EVALUATION OF AN AUTOMATED HIGH SENSITIVITY AMINO ACID ANALYZER BASED ON THE 9-FLUORENYLMETHYLCHLOROFORMATE (FMOCI) CHEMISTRY, Pages 255-265, Alan J. Smith, Jack M. Presley, William McIntire
26 - SIMPLE, ECONOMICAL AMINO ACID ANALYSIS BASED ON PRE-COLUMN DERIVATIVIZATION WITH 9-FLUORENYLMETHYL CHLOROFORMATE (FMOC), Pages 266-272, James E. Carlton, William T. Morgan
27 - SUCCESSFUL PTC AMINO ACID ANALYSIS AT THE PICOMOLE LEVEL, Pages 273-283, Donna Atherton
28 - A COMPREHENSIVE APPROACH TO AMINO ACID ANALYSIS, Pages 284-294, David R. Dupont, Pamela S. Keim, Arthur H. Chui, Rose Bello, MeriLisa Bozzini, Kenneth J. Wilson
29 - AUTOMATIC HYDROLYSIS AND PTC AMINO ACID ANALYSIS A PROGRESS REPORT, Pages 295-304, Karen A. West, John W. Crabb
30 - AMINO ACID ANALYSIS AT THE FEMTOMOLE LEVEL USING THE DIMETHYLAMINOAZOBENZENE SULFONYL CHLORIDE PRECOLUMN DERIVATIZATION METHOD: Potential and limitation, Pages 305-314, J.-Y. CHANG, R. KNECHT, P. JENOE, S. VEKEMANS
31 - DETECTION OF NORLEUCINE INCORPORATION INTO RECOMBINANT PROTEINS SYNTHESIZED IN E. COLI, Pages 315-326, Bernard N. Violand, Ned R. Siegel, Gregg Bogosian, Wesley E. Workman, James F. Kane
32 - OCCURRENCE OF METHIONINE SULFOXIDE DURING PRODUCTION OF RECOMBINANT HUMAN INSULIN-LIKE GROWTH FACTOR I (IGF-I), Pages 327-333, Maris G.N. Hartmanis, Åke Engström
Introduction: SECTION IV. HPLC TECHNIQUES APPLIED TO PROTEINS, Pages 337-338, Kenneth J. Wilson
33 - TECHNIQUES IN PROTEIN CHEMISTRY OPTIMIZATION OF MONOCLONAL ANTIBODY PURIFICATION, Pages 339-347, David R. Nau Ph.D.
34 - NEW HYDROPHILIC POLYMER FOR PROTEIN SEPARATIONS BY HPLC, Pages 348-356, Naotaka Kitagawa
35 - EFFECTS OF pH AND ION PAIRING AGENTS ON THE REVERSE PHASE HPLC OF HYDROPHILIC PEPTIDES: A CASE STUDY OF PENTIGETIDE AND ITS FRAGMENTS, Pages 357-363, Gobi R. Nagarajan, Jo-Lynne S. Boone, Thomas J. Stolzer, Steven P. Richieri
36 - CHROMATOGRAPHIC ANALYSIS OF GLYCOPROTEIN DERIVED CARBOHYDRATES: PELLICULAR ION EXCHANGE RESINS AND PULSED ELECTROCHEMICAL DETECTION, Pages 364-376, Joseph D. Olechno, Steven R. Carter, William T. Edwards, Dennis G. Gillen, R. Reid Townsend, Y.C. Lee, Mark R. Hardy
37 - ENZYMATIC DIGESTION OF PROTEINS AND HPLC PEPTIDE ISOLATION IN THE SUB-NANOMOLE RANGE, Pages 377-391, Kathryn L. Stone, Mary B. LoPresti, Nancy D. Williams, J. Myron Crawford, Raymond DeAngelis, Kenneth R. Williams
38 - THE USE OF GLASS CAPILLARY TUBES AS DISPOSABLE MICROBORE COLUMNS FOR RP-HPLC OF PROTEINS AND PEPTIDES, Pages 392-398, Christopher Southan
39 - ANALYSIS OF PEPTIDES AT LOW LEVELS USING A CHROMATOGRAPHY SYSTEM OPTIMIZED FOR NARROW-BORE COLUMNS, Pages 399-413, Robert Burgoyne, Catherine Stacey, Patricia Young, Nancy Astephen, Michael Merion
Introduction: SECTION V PROTEIN AND PEPTIDES: GENERAL TECHNIQUES, Pages 417-419, Albert Light, Robert F. Pfeifer
40 - RAPID TWO-STEP PURIFICATION OF HUMAN PLACENTAL ESTROGEN SYNTHETASE BY SUBSTRATE-SPECIFIC AFFINITY CHROMATOGRAPHY, Pages 420-429, L. Tan
41 - COMPUTER ASSISTED DESIGN OF RECOGNITION PEPTIDES, Pages 430-438, James G. Omichinski, Arthur D. Olson, Snorri S. Thorgeirsson, Giorgio Fassina
42 - NON–CHROMATOGRAPHIC SEPARATION OF PEPTIDES, Pages 439-447, Sherrell L. Early, Sheila G. Magil, Curt Novak, Marcus J. Horn
43 - AN ENDOGENOUS BACTERIAL PROTEASE SPECIFICALLY CLEAVES A FUSION PROTEIN JUNCTION CONSISTING OF CONSECUTIVE BASIC AMINO ACIDS TO GENERATE A BIOLOGICALLY-ACTIVE EUKARYOTIC ADHESIVE PROTEIN, BINDIN, Pages 448-455, Charles G. Glabe, Sandra Brockman, Angelika Lopez, Ken Kimura, Laura Kennedy, Paul L. DeAngelis
44 - A RAPID PROCEDURE FOR THE QUANTITATIVE ANALYSIS OF MONOCLONAL ANTIBODIES BY HIGH PERFORMANCE CAPILARY ELECTOPHORESIS, Pages 456-467, Norberto A. Guzman, Luis Hernandez
45 - FACTORS AFFECTING PLATE HEIGHT IN HIGH PERFORMANCE ZONAL CAPILLARY ELECTROPHORESES (HPZCE), Pages 468-478, D. McManigill, S.A. Swedberg
46 - ANALYSIS OF PROTEINS IN BRONCHOALVEOLAR LAVAGE FLUIDS DURING PULMONARY EDEMA RESULTING FROM NITROGEN DIOXIDE AND CADMIUM EXPOSURE, Pages 479-489, L.R. Gurley, J.E. London, L.A. Dethloff, D.M. Stavert, B.E. Lehnert
47 - A NEW ASSAY FOR ENTEROKINASE, Pages 490-496, Hanna Janska, Albert Light
48 - MEETING THE CHALLENGE IN PEPTIDE FRAGMENT PURIFICATION FOR PROTEIN SEQUENCING, Pages 497-505, TIMOTHY D. SCHLABACH, JOEL C. COLBURN, ROBERT J. MATTALIANO, SYLVIA YUEN
49 - Fmoc Chemistry: Synthesis, Kinetics, Cleavage, and Deprotection of Arginine-Containing Peptides, Pages 506-516, Jeanette L. Harrison, Gordon M. Petrie, Richard L. Noble, Hal S. Beilan, Sarah N. McCurdy, Alan R. Culwell
50 - SALT BRIDGE CONVERSION TO AMIDE BONDS WITH C2N2, Pages 517-525, Richard A. Day, Jane Kirley, Rebecca Tharp, David Ficker, Candace Strange, Ghiorgis Ghenbot
51 - THERMODYNAMIC BOOKKEEPING, Pages 526-530, W. Terry Jenkins
52 - INVESTIGATION OF HELIX DIPOLE AND SALT BRIDGE STABILIZATION OF SYNTHETIC HELICAL PEPTIDES USING CD AND NMR, Pages 531-546, Erin K. Bradley, John F. Thomason, Fred E. Cohen, Irwin D. Kuntz
Introduction: THE STP-3 SAMPLE WORKSHOP, Pages 549-551, Jean Rivier
53 - DESIGN AND SYNTHESIS OF SYMPOSIUM TEST PEPTIDE-3 (STP-3), Pages 552-559, Carl Hoeger, Robert Galyean, Richard McClintock, John Dykert, N. Leo Benoiton, Jean Rivier
54 - STRUCTURE DETERMINATION OF STP-3, Pages 560-568, Stanley A. Hefta, Marc J. Besman, Terry D. Lee, John E. Shively, Raymond J. Paxton
55 - STRUCTURE OF SYMPOSIUM TEST PEPTIDE-3, Pages 569-579, James Elliott, Myron Crawford, Kathryn Stone, Janet Kapouch, William Roberts, Eric Jacobsen, Mary LoPresti, Kenneth Williams, Ray De Angelis, Walter J. McMurray, Chin-Kai Meng, Matthias Mann, John Fenn
56 - CHARACTERIZATION OF SYMPOSIUM TEST PEPTIDE-3, Pages 580-588, Donald F. Hunt, Patrick R. Griffin, John R. Yates III, Jeffrey Shabanowitz, Jay W. Fox, Linda K. Beggerly
57 - CHARACTERIZATION AND SYNTHESIS OF STP-3, Pages 589-597, Sylvia W. Yuen, Kenneth M. Otteson, Joel C. Colburn, William T. Moore, Tim D. Schlabach, David R. Dupont, Robert J. Mattaliano
Index, Pages 599-612