Super-resolution microscopy : methods and protocols

Front Matter ....Pages i-xi
 Super-Resolution Microscopy Techniques and Their Potential for Applications in Radiation Biophysics  (Jan Philipp Eberle, Alexander Rapp, Matthias Krufczik, Marion Eryilmaz, Manuel Gunkel, Holger Erfle et al.)....Pages 1-13
 Managing the Introduction of Super-Resolution Microscopy into a Core Facility  (Jeffrey A. Kamykowski, Brian Storrie)....Pages 15-19
 Live-Cell STED Imaging with the HyPer2 Biosensor  (Natalia M. Mishina, Vsevolod V. Belousov)....Pages 21-28
 Diffraction-Unlimited Fluorescence Imaging with an EasySTED Retrofitted Confocal Microscope  (André Klauss, Carsten Hille)....Pages 29-44
 Two-Photon STED Microscopy for Nanoscale Imaging of Neural Morphology In Vivo  (Mirelle J. T. ter Veer, Thomas Pfeiffer, U. Valentin Nägerl)....Pages 45-64
 STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells  (Roman S. Erdmann, Derek Toomre, Alanna Schepartz)....Pages 65-78
 Four-Channel Super-Resolution Imaging by 3-D Structured Illumination  (Ulrike Engel)....Pages 79-94
 Correlative SIM-STORM Microscopy  (O. Burri, T. Laroche, R. Guiet, A. Seitz)....Pages 95-103
 Correlative Super-Resolution Fluorescence Imaging and Atomic Force Microscopy for the Characterization of Biological Samples  (Patricia Bondia, Santiago Casado, Cristina Flors)....Pages 105-113
 Quantitative Single-Molecule Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic Analysis of Fluorophore Blinking Cycles  (Franziska Fricke, Joel Beaudouin, Sebastian Malkusch, Roland Eils, Mike Heilemann)....Pages 115-126
 Two-Color Single-Molecule Tracking in Live Cells  (Siegfried Hänselmann, Dirk-Peter Herten)....Pages 127-138
 Fully Automated Targeted Confocal and Single-Molecule Localization Microscopy  (Jan Philipp Eberle, Walter Muranyi, Holger Erfle, Manuel Gunkel)....Pages 139-152
 Brain Slice Staining and Preparation for Three-Dimensional Super-Resolution Microscopy  (Christopher L. German, Manasa V. Gudheti, Annette E. Fleckenstein, Erik M. Jorgensen)....Pages 153-162
 Correlative In-Resin Super-Resolution Fluorescence and Electron Microscopy of Cultured Cells  (Errin Johnson, Rainer Kaufmann)....Pages 163-177
 Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments  (Jonathan B. Grimm, Timothy A. Brown, Brian P. English, Timothée Lionnet, Luke D. Lavis)....Pages 179-188
 Measuring Nanometer Distances Between Fluorescent Labels Step-by-Step  (Susanna Maria Früh, Ingmar Schoen)....Pages 189-203
 Correlative Single-Molecule Localization Microscopy and Confocal Microscopy  (Christian Soeller, Yufeng Hou, Isuru D. Jayasinghe, David Baddeley, David Crossman)....Pages 205-217
 Correlative Fluorescence Super-Resolution Localization Microscopy and Platinum Replica EM on Unroofed Cells  (Kem A. Sochacki, Justin W. Taraska)....Pages 219-230
 In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT  (Brian J. Beliveau, Alistair N. Boettiger, Guy Nir, Bogdan Bintu, Peng Yin, Xiaowei Zhuang et al.)....Pages 231-252
 Super-Resolution High Content Screening and Analysis  (K. Soliman)....Pages 253-259
Back Matter ....Pages 261-262