Subtilisin Enzymes: Practical Protein Engineering

Front Matter....Pages i-ix
The Complex Between Mesentericopeptidase and Eglin-C....Pages 1-4
Crystallographic Study of Eglin-C Binding to Thermitase....Pages 5-9
Design of Specific Peptide Structures and Subtilisin Enzyme Inhibitors Using α, β-Dehydro-Residues....Pages 11-20
Structure and Function of Subtilisin BPN’ as Studied Through Crystallographic Studies on a Series of its Complexes with Genetically Engineered Proteinaceous Inhibitor SSI....Pages 21-27
Three Dimensional Structure of the Antibiotic Bacitracin a Complexed to Two Different Subtilisin Proteases: Novel Mode of Enzyme Inhibition....Pages 29-41
Active Site Binding Loop Stabilization in the Subtilisin Inhibitor Eglin C: Structural and Functional Studies on Specifically Designed Mutants in Complex with Subtilisin and the Uncomplexed Inhibitor....Pages 43-47
Crystal Structures of the Alkaline Proteases Savinase and Esperase from Bacillus Lentus ....Pages 49-61
Modelling and Engineering of Enzyme/Substrate Interactions in Subtilisin-Like Enzymes of Unknown 3-Dimensional Structure....Pages 63-73
Subtilases: Subtilisin-Like Serine Proteases....Pages 75-93
Cloning and Expression of the Gene Encoding a Novel Proteinase from TritiraChium Album Limber....Pages 95-104
Studies of Binding Sites in the Subtilisin from Bacillus Lentus by Means of Site Directed Mutagenesis and Kinetic Investigations....Pages 105-112
Laundry Performance Of Subtilisin Proteases....Pages 113-120
Free Energy Perturbation Techniques Applied To Subtilisin BPN’ Stability....Pages 121-131
Thermitase — Kinetic Differentiation to the Subtilisins....Pages 133-140
Fluorescence Decay of Tryptophans in Serine Proteinases from Microorganisms: Relation to X-Ray Models....Pages 141-145
Propeptide-Mediated Folding in Subtilisin: The Intramolecular Chaperone Concept....Pages 147-154
Roles of The N- and C- Terminal Pro-Sequences of Aqualysin I Precursor in the Processing and Extracellular Secretion of the Enzyme....Pages 155-157
Crystal Structure Analysis of Subtilisin BPN’ Mutants Engineered for Studying Thermal Stability....Pages 159-169
Random Mutagenesis of the Weak Calcium Binding Site in Subtilisin Carlsberg and Screening for Thermostability by Temperature-Gradient Gel Electrophoresis....Pages 171-182
Inhibition of Proteinase K by Mercury....Pages 183-189
Crystallographic Investigations of Subtilisin BPN’ Mutants Engineered for Studying Thermal Stability....Pages 191-201
Structural and Functional Consequences of Engineering the High Alkaline Serine Protease PB92....Pages 203-218
Engineering Surface Charges in a Subtilisin....Pages 219-228
PH Dependence of the Catalytic Activity of a Subtilisin-Like Proteinase....Pages 229-233
An Investigation of the Savinase Water Channel: Implications of Cavity Mutations....Pages 235-241
Substrate Specificity of Natural Variants and Genetically Engineered Intermediates of Bacillus Lentus Alkaline Proteases....Pages 243-256
Unusual Ligand Binding at the Active Site Domain of an Engineered Mutant of Subtilisin BL....Pages 257-268
Studies on the Structure and Function of Subtilisin E by Protein Engineering....Pages 269-275
Structural Changes Leading to Increased Enzymatic Activity in an Engineered Variant of Bacillus Lentus Subtilisin....Pages 277-283
Back Matter....Pages 285-288