Subchronic Inhalation Toxicity 90-Day Study

OECD GUIDELINE FOR THE TESTING OF CHEMICALS
SUMMARY
This revised Test Guideline 413 (TG 413) has been designed to fully characterize test article toxicity by the inhalation route for a subchronic duration (90 days), and to provide robust data for quantitative inhalation risk assessments.  Groups of 10 ...
INTRODUCTION
INITIAL CONSIDERATIONS
DESCRIPTION OF THE METHOD
	Selection of Animal Species
	Preparation of Animals
	Animal Husbandry
	Inhalation Chambers
TOXICITY STUDIES
	Limit Concentrations
	Range-Finding Study
	Main Study
	Interim Sacrifices
	Satellite (Reversibility) Study
	Control Animals
EXPOSURE CONDITIONS
	Administration of Concentrations
19. Animals are exposed to the test article as a gas, vapour, aerosol, or a mixture thereof.  The physical state to be tested depends on the physico-chemical properties of the test article, the selected concentrations, and/or the physical form most li...
	Particle-Size Distribution
	Test Article Preparation in a Vehicle
MONITORING OF EXPOSURE CONDITIONS
	Chamber Airflow
	Chamber Temperature and Relative Humidity
	Test Article:  Nominal Concentration
	Test Article:  Actual Concentration
	Test Article:  Particle Size Distribution
OBSERVATIONS
BODY WEIGHTS
FOOD AND WATER CONSUMPTION
CLINICAL PATHOLOGY
OPHTHALMOLOGICAL EXAMINATION
GROSS PATHOLOGY AND ORGAN WEIGHTS
HISTOPATHOLOGY
DATA AND REPORTING
	Data
	Test Report
		Test animals and husbandry
		Test article
		Vehicle
		Inhalation chamber
		Exposure data
		Test conditions
		Results
		Discussion and interpretation of results