Structural and Contractile Proteins Part C: The Contractile Apparatus and the Cytoskeleton

Content: 
Contributors to volume 134
Pages xi-xv

Preface
Pages xvii-xviii
Richard B. Vallee

Volumes in series
Pages xix-xxxi

[1] Preparation and assay of cytoplasmic and secreted gelsolin Original Research Article
Pages 3-9
H.L. Yin

[2] Purification of severin Original Research Article
Pages 9-13
Susan S. Brown

[3] Isolation of fascin, and actin-bundling protein, and SU45, an actin-severing/capping protein from sea urchin eggs Original Research Article
Pages 13-23
Joseph Bryan

[4] Purification of the intestnal microvillus cytoskeletal proteins villin, fimbrin, and ezrin Original Research Article
Pages 24-37
Anthony Bretscher

[5] Purification of caldesmon Original Research Article
Pages 37-42
William Lynch, Anthony Bretscher

[6] Purification of fodrin from mammalian brain Original Research Article
Pages 42-54
Richard Cheney, Joel Levine, Mark Willard

[7] Purification of brain analogs of red blood cell membrane skeletal proteins: Ankyrin, protein 4.1 (synapsin), spectrin, and spectrin subunits Original Research Article
Pages 55-69
Vann Bennett, Anthony J. Baines, Jonathan Davis

[8] Purification and assay of vinculin, metavinculin, and talin Original Research Article
Pages 69-77
T. O'Halloran, L. Molonoy, K. Burridge

[9] Isolation of metavinculin from chicken smooth muscle Original Research Article
Pages 78-85
Janet D'Angelo Siliciano, Susan W. Craig

[10] Reversible assembly purification of microtubules without assemly-promoting agents and further purification of tubulijn, microtubule-associated proteins, and MAP fragments Original Research Article
Pages 89-104
Richard B. Vallee

[11] Purification of brain microtubules and microtubule-associated protein 1 using taxol Original Research Article
Pages 104-115
Richard B. Vallee

[12] Purification of microtubules and microtubule-associated proteins from sea urchin eggs and cultured mammalian cells using taxol, and use of exogenous taxol-stabilized brain microtubules for purifying microtubule-associated proteins Original Research Article
Pages 116-127
Richard B. Vallee, Christine A. Collins

[13] Isolation of sea urchin egg tubulin Original Research Article
Pages 128-138
H. William Detrich III

[14] Direct identification of microtubule-associated proteins be selective extraction of cultured cells Original Research Article
Pages 139-147
Frank Solomon

[15] Purification and assay of primate 210K microtubule-associated protein Original Research Article
Pages 147-156
Jeannette ChloГ« Bulinski

[16] Purification of tau protein from brain Original Research Article
Pages 156-160
David Drubin, Marc Kirschner

[17] Purification and assay of cold-stable microtubules and STOP protein Original Research Article
Pages 160-170
Robert L. Margolis, Charles T. Rauch, Didier Job

[18] Isolation and purification of tubulin tyrosine ligase Original Research Article
Pages 170-179
Jürgen Wehland, Heinz C. Schröder, Klaus Weber

[19] Proteolytic modification of tubulin Original Research Article
Pages 179-190
Luis Serrano, Francisco Wandosell, Javier de la Torre, JesГєs Avila

[20] Isolation of sea urchin spindles and cytasters Original Research Article
Pages 190-199
Ryoko Kuriyama

[21] Isolation of native, membrane-containing mitotic apparatus from sea urchin embryos Original Research Article
Pages 200-217
Robert B. Silver

[22] Isolation of mitotic spindles from mammalian cells Original Research Article
Pages 217-225
R.C. Brady, M.J. Schibler, F. Cabral

[23] Isolation of mitotic spindles and spindle pole bodies from yeast Original Research Article
Pages 225-232
Jeremy S. Hyams, Stephen M. King

[24] Isolation of the erythrocyte marginal band Original Research Article
Pages 232-252
William D. Cohen, Mary F. Ginsburg

[25] Isolation of basal bodies from Chlamydomonas Original Research Article
Pages 252-261
William J. Snell

[26] Isolation of mammalian centrosomes Original Research Article
Pages 261-268
T.J. Mitchison, M.W. Kirschner

[27] Isolation of a kinetochore—centromere fraction from HeLa metaphase chromosomes Original Research Article
Pages 268-280
Manuel M. Valdivia, B.R. Brinkley

[28] Isolation of Chlamydomonas flagella and flagellar axonemes Original Research Article
Pages 280-290
George B. Witman

[29] Purification and characterization of Chlamydomonas flagellar dyneins Original Research Article
Pages 291-306
Stephen M. King, Tim Otter, George B. Witman

[30] Preparation and properties of dynein from Tetrahymena cilia Original Research Article
Pages 306-317
Kenneth A. Johnson

[31] Extraction and properties of dyneins from bull spermatozoa Original Research Article
Pages 318-324
Claude Gagnon

[32] Purification of cytoplasmic dynein from Strongylocentrotus sea urchin eggs Original Research Article
Pages 325-337
M.M. Pratt

[33] Purification of cytoplasmic dynein from sea urchin eggs Original Research Article
Pages 337-351
Shin-ichi Hisanaga, Hikoichi Sakai

[34] Separation of cytokeratin polypeptides by gel electrophoretic and chromatographic techniques and their identification by immunoblotting Original Research Article
Pages 355-371
Thomas Achtstaetter, Mechthild Hatzfeld, Roy A. Quinlan, David C. Parmelee, Werner W. Franke

[35] Isolation of intermediate filaments from neuronal tissues by cycles of assembly/disassembly Original Research Article
Pages 371-379
Robert V. Zackroff

[36] Purification of neurofilaments and their constituent polypeptides Original Research Article
Pages 380-388
Ronald K.H. Liem

[37] Isolation of paired helical filaments and amyloid fibers from human brain Original Research Article
Pages 388-404
Dennis J. Selkoe, Carmela R. Abraham

[38] Methods for studying spasmin Original Research Article
Pages 404-408
W. Brad Amos

[39] Isolation of the calcium-modulated contractile protein of striated flagellar roots Original Research Article
Pages 408-414
J.L. Salisbury, K.W. Aebig, D.E. Coling

[40] Isolation of nematode major sperm proteins Original Research Article
Pages 414-420
Samuel Ward, Michael R. Klass

[41] Antibody inhibitors of nonmuscle myosin function and assembly Original Research Article
Pages 423-453
Daniel P. Kiehart, Donald A. Kaiser, Thomas D. Pollard

[42] Preparation of antibodies reactive with specific regions of cytoskeletal proteins Original Research Article
Pages 453-460
Jeannette ChloГ« Bulinski, Gregg G. Gundersen

[43] Purification of isoform-selective actin antibody from polyclonal antiserum Original Research Article
Pages 460-467
Susan W. Craig, Sheila K. Sanders, JosГ©V. Pardo

[44] Analysis of cytoskeletal structures using blot-purified monospecific antibodies Original Research Article
Pages 467-472
J.B. Olmsted

[45] Preparation of N-ethylmaleimide-modified heavy meromyosin and its use as a functional probe of actomyosin-based motility Original Research Article
Pages 473-477
W. Zacheus Cande

[46] Erythro-9-[3-(2-hydroxynonyl)]adenine and Vanadate as probes for microtubule-based cytoskeletal mechanochemistry Original Research Article
Pages 477-487
Stephen M. Penningroth

[47] Preparation of a fluorescent analog: Acetamidofluoresceinyl-labeled Dictyostelium discoideum О±-actinin Original Research Article
Pages 487-507
John R. Simon, D. Lansing Taylor

[48] Preparation, assay, and microinjection of fluorescently labeled cytoskeletal proteins: Actin, О±-actinin, and vinculin Original Research Article
Pages 507-519
Thomas E. Kreis

[49] Preparation and characterization of fluorescent analogs of tubulin Original Research Article
Pages 519-528
Patricia Wadsworth, E.D. Salmon

[50] Myosin movement in Vitro: A quantitative assay using oriented actin cables from Nitella Original Research Article
Pages 531-544
Michael P. Sheetz, Steven M. Block, James A. Spudich

[51] Methods to study the microtubule-neurofilament network in Vitro Original Research Article
Pages 544-555
Eric J. Aamodt, Robley C. Williams Jr.

[52] Gel overlay methods for detecting specific protein-protein interactions Original Research Article
Pages 555-560
Joann J. Otto

[53] Viewing single microtubules by video light microscopy Original Research Article
Pages 561-573
Bruce J. Schnapp

[54] Agar overlay method: High-resolution immunofluorescence for the study of the contractile apparatus Original Research Article
Pages 573-580
Yoshio Fukui, Shigehiko Yumura, Toshiko K. Yumura, Hiroshi Mori

[55] Polyethylene glycol embedding protocol for immunofluorescence microscopy Original Research Article
Pages 580-591
John J. Wolosewick

[56] Preembedding for localization by electron microscopy of cytoskeletal antigens in cultured cell monolayers using gold-labeled antibodies Original Research Article
Pages 592-597
J. De Mey, G. Langanger, G. Geuens, R. Nuydens, M. De Brabander

[57] Quick freeze, deep etch of the cytoskeleton Original Research Article
Pages 598-612
Nobutaka Hirokawa

[58] Cryo-electron microscopy of unstained frozen-hydrated microtubules Original Research Article
Pages 612-633
Eva-Maria Mandelkow, Eckhard Mandelkow

[59] X-ray diffraction of cytoskeletal fibers Original Research Article
Pages 633-657
Eckhard Mandelkow

[60] Time-resolved x-ray scattering of microtubule assembly using synchrotron radiation Original Research Article
Pages 657-676
Eckhard Mandelkow, Joan Bordas

[61] Rapid kinetic analysis of mechanochemical adenosinetriphosphatases Original Research Article
Pages 677-705
Kenneth A. Johnson

Author index
Pages 707-727

Subject index
Pages 729-748