Structural and Contractile Proteins Part B: The Contractile Apparatus and the Cytoskeleton

Content: 
Contributors to volume 85
Pages ix-xii

Preface
Page xiii
Dixie W. Frederiksen, Leon W. Cunningham

Volumes in series
Pages xviii-xxiii

[1] Introduction
Pages 1-6
John Gergely

[2] Preparation of myofibrils Original Research Article
Pages 9-12
Peter J. Knight, John A. Trinick

[3] Preparation of native thin filaments Original Research Article
Pages 12-15
Peter J. Knight, Stephen J. Lovell

[4] Hybridization and reconstitution of the thin filament Original Research Article
Pages 15-17
John M. Murray

[5] Preparation of native thick filaments Original Research Article
Pages 17-20
John Atrinick

[6] Hybridization and reconstitution of thick-filament structure Original Research Article
Pages 20-55
Jane F. Koretz

[7] Preparation of myosin and its subfragments from rabbit skeletal muscle Original Research Article
Pages 55-71
Sarkis S. Margossian, Susan Lowey

[8] Preparation and fractionation of myosin light chains and exchange of the essential light chains Original Research Article
Pages 72-81
Paul D. Wagner

[9] Preparation of light chains from scallop myosin Original Research Article
Pages 81-84
Andrew G. Szent-Györgyi, Regina Niebieski

[10] Sulfhydryl modification and labeling of myosin Original Research Article
Pages 84-93
Emil Reisler

[11] Chemical modification of myosin by active-site trapping of metal-nucleotides with thiol crosslinking reagents Original Research Article
Pages 93-116
James A. Wells, Ralph G. Yount

[12] Myosin active-site trapping with vanadate ion Original Research Article
Pages 116-123
Charles C. Goodno

[13] Assays for myosin Original Research Article
Pages 123-130
Thomas D. Pollard

[14] Preparation of C-Protein, H-Protein, X-Protein, and phosphofructokinase Original Research Article
Pages 130-138
Roger Starr, Gerald Offer

[15] Preparation and properties of M-line proteins Original Research Article
Pages 139-149
Hans M. Eppenberger, Emanuel Strehler

[16] Preparation and assay of paramyosin Original Research Article
Pages 149-160
Rhea J.C. Levine, Myra J. Elfvin, Vitaly Sawyna

[17] Preparation problems unique to Mercenaria paramyosin Original Research Article
Pages 160-164
Sonja Krause, Nancy Letko Munson

[18] Purification of muscle actin Original Research Article
Pages 164-181
Joel D. Pardee, James Aspudich

[19] Methods to measure actin polymerization Original Research Article
Pages 182-210
John A. Cooper, Thomas D. Pollard

[20] Methods to characterize actin filament networks Original Research Article
Pages 211-233
Thomas D. Pollard, John A. Cooper

[21] Preparation and identification of О±- and ОІ-tropomyosins Original Research Article
Pages 234-241
L.B. Smillie

[22] Preparation of troponin and its subnits Original Research Article
Pages 241-263
James D. Potter

[23] Purification of titin and nebulin Original Research Article
Pages 264-274
Kuan Wang

[24] Preparation of isolated thick and thin filaments Original Research Article
Pages 277-284
Peter Cooke

[25] Preparation of individual smooth muscle cells from the stomach of Bufo marinus Original Research Article
Pages 284-292
F.S. Fay, R. Hoffmann, S. Leclair, P. Merriam

[26] Preparation of smooth muscle myosin Original Research Article
Pages 292-298
Dixie W. Frederiksen, Dianne Drees

[27] Purification of smooth muscle myosin light-chain kinase Original Research Article
Pages 298-308
Robert S. Adelstein, Claude B. Klee

[28] Purification of smooth muscle phosphatases Original Research Article
Pages 308-315
Mary Pato, Robert S. Adelstein

[29] Preparation of smooth muscle О±-actinin Original Research Article
Pages 316-321
Susan W. Craig, Christine L. Lancashire, John A. Cooper

[30] Purification and properties of avian and mammalian filamins Original Research Article
Pages 322-328
Peter J.A. Davies, Yutaka Shizuta, Ira Pastan

[31] Purification of nonmuscle myosins Original Research Article
Pages 331-356
Thomas D. Pollard

[32] Myosins from Acanthamoeba castellanii Original Research Article
Pages 357-363
Edward D. Korn, Jimmy H. Collins, Hiroshi Maruta

[33] Phosphorylation of nonmuscle myosin and stabilization of thick filament structure Original Research Article
Pages 364-370
John Kendrick-Jones, K.A. Taylor, J.M. Scholey

[34] Preparation of cytoplasmic actin Original Research Article
Pages 371-373
Jerry P. Weir, Dixie W. Frederiksen

[35] Cytoplasmic tropomyosins Original Research Article
Pages 373-375
Isaac Cohen

[36] Preparation of tubulin from brain Original Research Article
Pages 376-385
Robley C. Williams Jr., James C. Lee

[37] Isolation of tubulin from nonneural sources Original Research Article
Pages 385-393
Kevin W. Farrell

[38]Physical properties of purified calf brain tubulin Original Research Article
Pages 393-408
George C. Na, Serge N. Timasheff

[39] Purification and assay of microtubule-associated proteins (MAPs) Original Research Article
Pages 409-416
Roger D. Sloboda, Joel L. Rosenbaum

[40] Characterization of tubulin and microtubule-associated protein interactions with guanine nucleotides and their nonhydrolyzable analogs Original Research Article
Pages 416-433
Daniel L. Purich, Brian J. Terry, Robert K. MacNeal, Timothy L. Karr

[41] Techniques for the study of microtubule assembly in vitro Original Research Article
Pages 433-439
Felicia Gaskin

[42] Microtubule disassembly: A quantitative kinetic approach for defining endwise linear depolymerization Original Research Article
Pages 439-450
Daniel L. Purich, Timothy L. Karr, David Kristofferson

[43] Preparation and purification of dynein Original Research Article
Pages 450-474
Christopher W. Bell, Clarence L. Fraser, Winfield S. Sale, Wending Y. Tang, I.R. Gibbons

[44] Preparation of spectrin Original Research Article
Pages 475-480
W.B. Gratzer

[45] Macrophage actin-binding protein Original Research Article
Pages 480-488
John H. Hartwig, Thomas P. Stossel

[46] Preparation and assay of the intermediate filament proteins desmin and vimentin Original Research Article
Pages 488-508
E. Lazarides, B.L. Granger

[47] Optical diffraction and filtering of electron micrographs of contractile proteins Original Research Article
Pages 511-514
Pauline M. Bennett

[48] Fluorescent localization of contractile proteins in tissue culture cells Original Research Article
Pages 514-562
Kuan Wang, James R. Feramisco, John F. Ash

[49] Preparation of contractile proteins for photon correlation spectroscopic and classical light-scattering studies Original Research Article
Pages 562-570
Charles Montague, Francis D. Carlson

[50] Hydrodynamic approaches to the study of high molecular weight proteins and large macromolecular assemblies Original Research Article
Pages 570-573
Walter F. Stafford III

[51] Fluorescence as a probe of contractile systems Original Research Article
Pages 574-593
Roger Cooke

[52] Electron paramagnetic resonance of contractile systems Original Research Article
Pages 594-624
John C. Seidel

[53] Phosphorus-31 nuclear magnetic resonance of contractile systems Original Research Article
Pages 624-676
Michael BГЎrГЎny, Thomas Glonek

[54] Optical activity measurements for elucidating structure—Function relationships in muscle protein systems Original Research Article
Pages 677-698
William D. McCubbin, Cyril M. Kay

[55] Special instrumentation and techniques for kinetic studies of contractile systems Original Research Article
Pages 698-708
Howard D. White

[56] Interaction of actin and myosin in the presence and the absence of ATP Original Research Article
Pages 709-724
Lois E. Greene, Evan Eisenberg

Author index
Pages 725-744

Subject index
Pages 745-774