Streptococcus pneumoniae: Methods and Protocols

Front Matter ....Pages i-xv
Front Matter ....Pages 1-1
 Optimal Conditions for Streptococcus pneumoniae Culture: In Solid and Liquid Media  (Norma Suárez, Esther Texeira)....Pages 3-10
Front Matter ....Pages 11-11
 Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell  (Sven Hammerschmidt, Manfred Rohde)....Pages 13-33
 Immunofluorescent Staining and High-Resolution Microscopy to Study the Pneumococcal Cell  (Federico Iovino, Birgitta Henriques-Normark)....Pages 35-39
 Construction of Fluorescent Pneumococci for In Vivo Imaging and Labeling of the Chromosome  (Morten Kjos)....Pages 41-51
 High-Resolution and Super-Resolution Immunofluorescent Microscopy Ex Vivo to Study Pneumococcal Interactions with the Host  (Federico Iovino, Birgitta Henriques-Normark)....Pages 53-59
Front Matter ....Pages 61-61
 Natural Genetic Transformation: A Direct Route to Easy Insertion of Chimeric Genes into the Pneumococcal Chromosome  (Isabelle Mortier-Barrière, Nathalie Campo, Mathieu A. Bergé, Marc Prudhomme, Patrice Polard)....Pages 63-78
 Gene Expression Analysis in the Pneumococcus  (Rory A. Eutsey, Carol A. Woolford, Surya D. Aggarwal, Rolando A. Cuevas, N. Luisa Hiller)....Pages 79-88
 Transcriptional Knockdown in Pneumococci Using CRISPR Interference  (Morten Kjos)....Pages 89-98
Front Matter ....Pages 99-99
 Protein Expression Analysis by Western Blot and Protein–Protein Interactions  (María Dolores Cima-Cabal, Fernando Vazquez, Juan R. de los Toyos, María del Mar García-Suárez)....Pages 101-111
 Mass Spectrometry to Study the Bacterial Proteome from a Single Colony  (Jianwei Zhou, Lu Zhang, Huixia Chuan, Angela Sloan, Raymond Tsang, Keding Cheng)....Pages 113-121
 Bead-Based Flow-Cytometric Cell Counting of Live and Dead Bacteria  (Fang Ou, Cushla McGoverin, Joni White, Simon Swift, Frédérique Vanholsbeeck)....Pages 123-134
Front Matter ....Pages 135-135
 In Vitro Adhesion, Invasion, and Transcytosis of Streptococcus pneumoniae with Host Cells  (Terry Brissac, Carlos J. Orihuela)....Pages 137-146
 Growing and Characterizing Biofilms Formed by Streptococcus pneumoniae  (Yashuan Chao, Caroline Bergenfelz, Anders P. Hakansson)....Pages 147-171
 In Vivo Mouse Models to Study Pneumococcal Host Interaction and Invasive Pneumococcal Disease  (Federico Iovino, Vicky Sender, Birgitta Henriques-Normark)....Pages 173-181
 Two-Photon Intravital Imaging of Leukocytes in the Trachea During Pneumococcal Infection  (Miguel Palomino-Segura, Santiago F. Gonzalez)....Pages 183-194
 IVIS Spectrum CT to Image the Progression of Pneumococcal Infections In Vivo  (Adam Sierakowiak, Birgitta Henriques-Normark, Federico Iovino)....Pages 195-202
Front Matter ....Pages 203-203
 The Pneumococcus and Its Critical Role in Public Health  (Godwin Oligbu, Norman K. Fry, Shamez N. Ladhani)....Pages 205-213
 The Epidemiology and Biostatistics of Pneumococcus  (Godwin Oligbu, Norman K. Fry, Shamez N. Ladhani)....Pages 215-224
Back Matter ....Pages 225-227