Skin Stem Cells: Methods and Protocols

Front Matter ....Pages i-xv
 In Vivo Genetic Alteration and Lineage Tracing of Single Stem Cells by Live Imaging  (Olivia Farrelly, Paola Kuri, Panteleimon Rompolas)....Pages 1-14
 Ex Vivo Imaging and Genetic Manipulation of Mouse Hair Follicle Bulge Stem Cells  (Daniel Haensel, Melissa A. McNeil, Xing Dai)....Pages 15-29
 Quantitative Detection of Low-Abundance Transcripts at Single-Cell Level in Human Epidermal Keratinocytes by Digital Droplet Reverse Transcription-Polymerase Chain Reaction  (Frédéric Auvré, Julien Coutier, Michèle T. Martin, Nicolas O. Fortunel)....Pages 31-41
 Qualitatively Monitoring Binding and Expression of the Transcription Factors Sp1 and NFI as a Useful Tool to Evaluate the Quality of Primary Cultured Epithelial Stem Cells in Tissue Reconstruction  (Gaëtan Le-Bel, Sergio Cortez Ghio, Danielle Larouche, Lucie Germain, Sylvain L. Guérin)....Pages 43-73
 Ribonucleoproteins Mediated Efficient In Vivo Gene Editing in Skin Stem Cells  (Wenbo Wu, Ting Chen)....Pages 75-86
 Isolation and Characterization of Cutaneous Epithelial Stem Cells  (Stephanie R. Gillespie, David M. Owens)....Pages 87-99
 Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin  (Mariana T. Cerqueira, Ana M. Frias, Rui L. Reis, Alexandra P. Marques)....Pages 101-110
 Whole-Mount Immunofluorescent Staining Coupled to Multicolor Lineage Tracing Model for Analyzing the Spatiotemporal Organization of Epidermal Stem Cells  (Edwige Roy, Kiarash Khosrotehrani)....Pages 111-118
 Isolation and Enrichment of Newborn and Adult Skin Stem Cells of the Interfollicular Epidermis  (Stefano Sol, Dario Antonini, Caterina Missero)....Pages 119-132
 Isolation and Cultivation of Epidermal (Stem) Cells  (Xusheng Wang, Shiyang Dong, Yaojiong Wu)....Pages 133-138
 One-Step Simple Isolation Method to Obtain Both Epidermal and Dermal Stem Cells from Human Skin Specimen  (Hua Qian, Xue Leng, Jie Wen, Qian Zhou, Xin Xu, Xunwei Wu)....Pages 139-148
 Isolation and Cultivation of Skin-Derived Precursors  (Xiaoxiao Wang, Shiyang Dong, Yaojiong Wu)....Pages 149-152
 Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells  (Petek Korkusuz, Sevil Köse, Nilgün Yersal, Selin Önen)....Pages 153-163
 Isolation of Human Skin Epidermal Stem Cells Based on the Expression of Endothelial Protein C Receptor  (Meilang Xue, Suat Dervish, Christopher J. Jackson)....Pages 165-174
 Decellularized bSIS-ECM as a Regenerative Biomaterial for Skin Wound Repair  (Mahmut Parmaksiz, Ayşe Eser Elçin, Yaşar Murat Elçin)....Pages 175-185
 Protocols for Full Thickness Skin Wound Repair Using Prevascularized Human Mesenchymal Stem Cell Sheet  (Lei Chen, Daniel Radke, Shaohai Qi, Feng Zhao)....Pages 187-200
 Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering  (Ehsan Taghiabadi, Bahareh Beiki, Nasser Aghdami, Amir Bajouri)....Pages 201-210
 Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration  (Ehsan Taghiabadi, Bahareh Beiki, Nasser Aghdami, Amir Bajouri)....Pages 211-219
 Skin Wound Healing: Refractory Wounds and Novel Solutions  (Gabriel M. Virador, Lola de Marcos, Victoria M. Virador)....Pages 221-241
 Isolation, Culture, and Motility Measurements of Epidermal Melanocytes from GFP-Expressing Reporter Mice  (Lina Dagnino, Melissa Crawford)....Pages 243-256
 Melanoblasts as Multipotent Cells in Murine Skin  (Tsutomu Motohashi, Takahiro Kunisada)....Pages 257-266
 Regeneration of Mouse Skin Melanocyte Stem Cells In Vivo and In Vitro  (Ke Yang, Weiming Qiu, Pei-Rong Gu, Mingxing Lei)....Pages 267-284
 Interactions Between Epidermal Keratinocytes, Dendritic Epidermal T-Cells, and Hair Follicle Stem Cells  (Krithika Badarinath, Abhik Dutta, Akshay Hegde, Neha Pincha, Rupali Gund, Colin Jamora)....Pages 285-297
 Isolating Immune Cells from Mouse Embryonic Skin  (Ambika S. Kurbet, Srikala Raghavan)....Pages 299-305
 Direct Conversion of Mouse Embryonic Fibroblasts into Neural Crest Cells  (Tsutomu Motohashi, Takahiro Kunisada)....Pages 307-321
 High-Titer Production of HIV-Based Lentiviral Vectors in Roller Bottles for Gene and Cell Therapy  (Hazal Banu Olgun, Hale M. Tasyurek, Ahter Dilsad Sanlioglu, Salih Sanlioglu)....Pages 323-345
 High-Grade Purification of Third-Generation HIV-Based Lentiviral Vectors by Anion Exchange Chromatography for Experimental Gene and Stem Cell Therapy Applications  (Hazal Banu Olgun, Hale M. Tasyurek, Ahter D. Sanlioglu, Salih Sanlioglu)....Pages 347-365
 Full-Thickness Human Skin Equivalent Models of Atopic Dermatitis  (Gopu Sriram, Paul Lorenz Bigliardi, Mei Bigliardi-Qi)....Pages 367-383
 Human Hair Follicle Associated-Pluripotent (hHAP) Stem Cells Differentiate to Cardiac Muscle Cells  (Robert M. Hoffman)....Pages 385-392
 Isolation of Normal Fibroblasts and Their Cancer-Associated Counterparts (CAFs) for Biomedical Research  (BarboraBarbora Dvořánková, Lukáš Lacina, Karel Smetana Jr.)....Pages 393-406
 Isolation of Cancer Stem Cells from Squamous Cell Carcinoma  (Silvia Fontenete, Mirna Perez-Moreno)....Pages 407-414
 Identification of Human Cutaneous Squamous Cell Carcinoma Cancer Stem Cells  (Carlotta Olivero, Huw Morgan, Girish K. Patel)....Pages 415-433
 Identification of Human Cutaneous Basal Cell Carcinoma Cancer Stem Cells  (Huw Morgan, Carlotta Olivero, Girish K. Patel)....Pages 435-450
Back Matter ....Pages 451-463