Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods

Content: 
Series Page Page ii  
Copyright Page iv  
Contributors Pages xi-xv  
Preface Pages xvii-xviii Maria Spies, Yann R. Chemla 
Chapter One - Direct Fluorescent Imaging of Translocation and Unwinding by Individual DNA Helicases Pages 1-32 T.L. Pavankumar, J.C. Exell, S.C. Kowalczykowski 
Chapter Two - Single-Molecule Imaging With One Color Fluorescence Pages 33-51 Y. Qiu, S. Myong 
Chapter Three - Measuring Membrane Protein Dimerization Equilibrium in Lipid Bilayers by Single-Molecule Fluorescence Microscopy Pages 53-82 R. Chadda, J.L. Robertson 
Chapter Four - Fluorescent Labeling of Proteins in Whole Cell Extracts for Single-Molecule Imaging Pages 83-104 S.R. Hansen, M.L. Rodgers, A.A. Hoskins 
Chapter Five - Quantifying the Assembly of Multicomponent Molecular Machines by Single-Molecule Total Internal Reflection Fluorescence Microscopy Pages 105-145 E.M. Boehm, S. Subramanyam, M. Ghoneim, M. Todd Washington, M. Spies 
Chapter Six - How to Measure Separations and Angles Between Intramolecular Fluorescent Markers Pages 147-185 K.I. Mortensen, J. Sung, J.A. Spudich, H. Flyvbjerg 
Chapter Seven - Precisely and Accurately Inferring Single-Molecule Rate Constants Pages 187-225 C.D. Kinz-Thompson, N.A. Bailey, R.L. Gonzalez Jr. 
Chapter Eight - Quantification of Functional Dynamics of Membrane Proteins Reconstituted in Nanodiscs Membranes by Single Turnover Functional Readout Pages 227-256 M.E. Moses, P. Hedegård, N.S. Hatzakis 
Chapter Nine - Putting Humpty–Dumpty Together: Clustering the Functional Dynamics of Single Biomolecular Machines Such as the Spliceosome Pages 257-283 C.E. Rohlman, M.R. Blanco, N.G. Walter 
Chapter Ten - Single-Molecule FRET to Measure Conformational Dynamics of DNA Mismatch Repair Proteins Pages 285-315 J.W. Gauer, S. LeBlanc, P. Hao, R. Qiu, B.C. Case, M. Sakato, M.M. Hingorani, D.A. Erie, K.R. Weninger 
Chapter Eleven - Single-Molecule Confocal FRET Microscopy to Dissect Conformational Changes in the Catalytic Cycle of DNA Topoisomerases Pages 317-351 S. Hartmann, D. Weidlich, D. Klostermeier 
Chapter Twelve - Probing the Conformational Landscape of DNA Polymerases Using Diffusion-Based Single-Molecule FRET Pages 353-378 J. Hohlbein, A.N. Kapanidis 
Chapter Thirteen - Methods for Investigating DNA Accessibility with Single Nucleosomes Pages 379-415 M.D. Gibson, M. Brehove, Y. Luo, J. North, M.G. Poirier 
Chapter Fourteen - Single-Molecule Fluorescence Studies of Fast Protein Folding Pages 417-459 Z. Wang, L.A. Campos, V. Muñoz 
Chapter Fifteen - Single-Molecule Multicolor FRET Assay for Studying Structural Dynamics of Biomolecules Pages 461-486 S. Lee, Y. Jang, S.-J. Lee, S. Hohng 
Chapter Sixteen - A Multicolor Single-Molecule FRET Approach to Study Protein Dynamics and Interactions Simultaneously Pages 487-516 M. Götz, P. Wortmann, S. Schmid, T. Hugel 
Chapter Seventeen - Interferometric Scattering Microscopy for the Study of Molecular Motors Pages 517-539 J. Andrecka, Y. Takagi, K.J. Mickolajczyk, L.G. Lippert, J.R. Sellers, W.O. Hancock, Y.E. Goldman, P. Kukura 
Chapter Eighteen - Enzyme Kinetics in Femtoliter Arrays Pages 541-560 P. Mogalisetti, D.R. Walt 
Author Index Pages 561-586  
Subject Index Pages 587-598