Signal Transduction — Single Cell Techniques

Front Matter....Pages I-XXVII
Front Matter....Pages 1-1
Maintaining Cells Under the Microscope....Pages 3-8
Simple Perfusion Chambers for Single Cell Measurements....Pages 9-18
Temperature Control on the Stage of the Microscope....Pages 19-23
A Modified U-Tube Drug Application System....Pages 24-30
Laser Microsurgery as a Tool in Single Cell Research....Pages 31-40
Front Matter....Pages 41-41
Significance of Ion Channels and Membrane Potential Changes in Cells....Pages 43-51
Practical Introduction to Patch Clamping by Simulation Experiments with Simple Electrical Circuits....Pages 52-87
Improved Electrophysiological Measurements by Series Resistance Compensation....Pages 88-98
Preparation of Patchable Plant Cell Protoplasts and a Procedure for the Improvement of Gigaseal Formation....Pages 99-108
Recording and Analysis of ATP-Sensitive Potassium Channels in Inside-Out Patches of Rabbit Ventricular Myocytes....Pages 111-124
Mechanotransduction and Mechanosensitive Ion Channels in Osteoblasts....Pages 125-134
The Study of (Plant) Ion Channels Reconstituted in Planar Lipid Bilayers....Pages 135-152
Ion Channel Measurement on the Cell Nucleus....Pages 153-170
Monitoring Cytosolic Calcium and Membrane Potential from Cell-Attached Patch-Clamp Currents in Human T Lymphocytes....Pages 171-184
Membrane Potential and Action Potential Measurements in Whole Cell and Perforated Patch Configurations....Pages 187-206
Perforated Patch-Clamp Technique in Heart Cells....Pages 207-213
Measurement and Analysis of Different Aspects of Potassium Currents in Human Lymphocytes....Pages 214-235
Ionic Conductances in Chicken Osteoclasts....Pages 236-245
GABA A Receptor-Mediated Chloride Currents in Acutely Dissociated Hippocampal Neurons....Pages 246-255
Measurement of Whole Cell Potassium Currents in Protoplasts from Tobacco Cell Suspensions....Pages 256-268
Front Matter....Pages 41-41
Inward Rectifying Potassium Conductance in Barley Aleurone Protoplasts....Pages 269-281
Front Matter....Pages 283-283
An Introduction to the Use of Fluorescent Probes in Ratiometric Fluorescence Microscopy....Pages 285-305
How To Use Video Cameras To Acquire Images....Pages 306-317
Strategies for Studying Intracellular pH Regulation....Pages 318-339
An Introduction to the Working Principles of the Flow Cytometer....Pages 343-347
Flow Cytometric Membrane Potential Measurements....Pages 348-357
Flow Cytometric Measurement of Cytosolic Calcium in Lymphocytes....Pages 358-372
Cytosolic pH and Cell Movement Measurement in Dictyostelium ....Pages 375-397
Analysis of Agonist-Induced Cell Recruitment in Terms of Intracellular Calcium Mobilization in a Population of Enzymatically Dispersed Pancreatic Acinar Cells....Pages 398-406
Quantitative Confocal Fluorescence Measurements in Living Tissue....Pages 409-441
Cytosolic Calcium Measurements with Confocal Microscopy....Pages 442-457
Back Matter....Pages 459-468