Separations Using Aqueous Phase Systems: Applications in Cell Biology and Biotechnology

Front Matter....Pages i-xvii
Front Matter....Pages 1-1
General Aspects of Aqueous Polymer Two-Phase Partition....Pages 3-5
Affinity Partitioning of Enzymes....Pages 7-14
Dye Affinity Partitioning of Serum Proteins....Pages 15-23
Hydrophobic Affinity Partitioning as a Method for Studying Conformational Changes in Proteins....Pages 25-31
Affinity Partition of Enzymes with Dextran-Bound Procion Yellow HE-3G: Effect of Ligand Density....Pages 33-34
Rapid Two-Phase Extraction of the Calvin Cycle Enzymes Aldolase and Phosphoglycerate Kinase....Pages 35-37
Affinity Partitioning of Phosphofructokinase from Rat Erythrocytes in Poly(Ethylene Glycol)-Cibacron Blue/Dextran Two-Phase Systems....Pages 39-40
Studies on Triazine Dye-Enzyme Interaction by Means of Affinity Partitioning....Pages 41-42
Front Matter....Pages 43-43
Separation of Organelles and Membranes by Phase Partitioning — A Short Overview....Pages 45-49
Plasma Membrane Isolation by Phase Partition using a Single Partition Step: A Tool for the in vivo Study of Lipid Transfer to the Plasma Membrane....Pages 51-58
Free-Flow Electrophoresis Resolves Plasma Membrane Vesicles Isolated by Aqueous Two Phase Partition into Two Populations of Different Sidedness....Pages 59-60
Raising Monoclonal Antibodies Against Plasma Membranes from Barley Embryos....Pages 61-62
Subfractionation of Cyanobacterial Thylakoids....Pages 63-64
Front Matter....Pages 65-65
Use of Phase Partitioning in Multidimensional Subcellular Fractionation....Pages 67-80
Comparison of Phase Partition with Alternative Methods for Isolation of Subcellular Structures....Pages 81-94
Analysis of the Domain Structure of Membranes by Fragmentation and Separation....Pages 95-99
Two-Phase Partitioning of Rat Liver Plasma Membranes....Pages 101-107
Plasma Membranes from Hepatomas and Cultured Cells by Aqueous Two-Phase Partition....Pages 109-110
Affinity Partitioning and Centrifugal Countercurrent Distribution of Synaptosomes from Calf Brain Cortex....Pages 111-112
Isolation of Plasma Membrane Vesicles from Fat Cells of Epididymal Fat Pads of the Rat by Aqueous Two-Phase Partition....Pages 113-115
Front Matter....Pages 117-117
Separation and Subfractionation of Cell Populations by Phase Partitioning — An Overview....Pages 119-125
Separation of Chondrocytes in Aqueous Two-Phase Systems....Pages 127-135
Problems of White Cell Separation Techniques in Relation to the Phase Partitioning of Neutrophils....Pages 137-144
T Cell Heterogeneity and Lymphoid Cell Populations in Normal Humans and Rheumatoid Arthritis Probed by Partitioning....Pages 145-148
Surface Characterization of Colloidal Drug Carriers for Drug Targeting by Aqueous Two-Phase Partitioning....Pages 149-155
Use of Partitioning to Study Differentiating Cells of Dictyostelium Discoideum ....Pages 157-164
Partition of Algal Cells of Various Strains in Aqueous Two Phase Systems....Pages 165-166
Antibiotic — Neutrophil Interactions Studied by Phase Partitioning....Pages 167-168
Surface Properties of Klebsiella and E Coli: Role of Capsular Polysaccharide in Protection Against Phagocytosis....Pages 169-170
Fractionation by Countercurrent Distribution (CCD) of Rat Erythrocytes During Animal Development....Pages 171-173
Attempts to Fractionate Rat Bone Marrow Cells by Countercurrent Distribution (CCD) in Dextran/Polyethylene Glycol Two-Phase Systems....Pages 175-177
The Partitioning of Liposomes of Defined Size and Composition....Pages 179-180
Front Matter....Pages 181-181
Second Immunoaffinity Ligands for Cell Separation....Pages 183-191
Cell Separation by Immunoaffinity Partition in Aqueous Polymer Two-Phase Systems....Pages 193-202
New Activated PEG Derivatives for Affinity Partitioning....Pages 203-210
Coupling of PEG to Proteins by Activation with Tresyl Chloride. Applications in Immunoaffinity Cell Partitioning....Pages 211-213
Front Matter....Pages 215-215
An Overview of Theoretical Developments for Protein Partitioning in Aqueous Two-Phase Polymer Systems....Pages 217-227
Protein Transport Processes in the Water-Water Interface in Incompatible Two Phase Systems....Pages 229-232
Water: The Third Component in Polymer Two-Phase Systems....Pages 233-238
Contact Angles as an Analytical Tool for Investigating Two-Phase Interactions with Biological Surfaces: A Review....Pages 239-247
Front Matter....Pages 215-215
Macrophage Surface Affinity Changes During Phagocytosis....Pages 249-259
A Polymer Mixing Approach to Chemoattractant-Stimulated Neutrophil Adhesion....Pages 261-270
Mechanism of Cell Partitioning; Equilibrium Contact Angle Measurements Predict Relative, Kinetic Partitioning Behaviour....Pages 271-280
Demixing Kinetics of Phase Separated Polymer Solutions in Microgravity....Pages 281-286
Dextran Coated Glass Slides as a Model for Cell Surface Interactions with Aqueous Two Phase Polymer Systems....Pages 287-288
A Thermodynamic Study of the Binding of the E. coli F41 Adhesin to its Receptor, Human Glycophorin....Pages 289-290
Temperature Dependence of the Phase Equilibria in the PEG-Dextran System — A Theoretical and Experimental Study....Pages 291-292
Front Matter....Pages 293-293
Large Scale Protein Recovery using Aqueous Phase Systems....Pages 295-297
Continuous Crosscurrent Extraction of Proteins in Process Scale....Pages 299-307
Aqueous Two-Phase Partition in Biochemical Recovery from Mammalian Cell Culture....Pages 309-315
Production of ß-Galactosidase Fused Protein A and the Purification of it in an Aqueous Two-Phase System....Pages 317-324
Continuous Affinity Partitioning in a Poly(Ethylene Glycol)-Reppal Pes Aqueous Two-Phase System....Pages 325-331
Fast Downstream Processing of Proteins by Flocculation and Liquid Extraction....Pages 333-335
Gravity Settling of APS — Characteristics and Process Applications....Pages 337-339
Improved Economics of Extractive Enzyme Recovery by Recycling: A Case Study....Pages 341-343
The use of PEG-NADH/Na 2 Co 3 Aqueous Two-Phase Systems for the Large-Scale Preparation of Technical Grade PEG-(M r 20,000)-N 6 -(2-Aminoethyl)-NADH....Pages 345-347
Front Matter....Pages 349-349
Extractive Bioconversions in Aqueous Phase Systems....Pages 351-360
Process Simulation of Continuous Bioconversions in Aqueous Two-Phase Systems....Pages 361-369
Basic Trials on Extractive Bioconversion of Glucose to Lactic Acid....Pages 371-375
Integration of Bioconversion and Down-Stream Processing — Starch Hydrolysis in an Aqueous Two-Phase System....Pages 377-378
Front Matter....Pages 379-379
Liquid-Liquid Partition Chromatography of Biopolymers in Aqueous Two-Phase Polymer Systems....Pages 381-392
Application of Liquid-Liquid Partition Chromatography (LLPC) in the Preparation of Steroid Binding Proteins....Pages 393-399
Column Based Liquid/Liquid Separation of Cells Using Aqueous Polymeric Two-Phase Systems....Pages 401-406
A New Approach to Countercurrent Distribution Combining Separation with Analysis in the Biomek Automated Laboratory Workstation....Pages 407-415
Elution Countercurrent Distribution....Pages 417-419
Gravimetric Analysis of Phase Systems using a Microwave Oven and the Rapid Determination of Phase Diagrams....Pages 421-422
Rapid Partitioning of Human Neutrophils by CCD....Pages 423-424
Quantitation of the Resolving Power of CCD using Human Erythrocytes....Pages 425-426
Front Matter....Pages 427-427
New Polymers for Aqueous Two-Phase Systems....Pages 429-438
Protein Partitioning Between Microemulsions and Conjugate Aqueous Phases....Pages 439-446
Immiscible Liquid Affinity Supports....Pages 447-453
Magnetically Enhanced Aqueous Two-Phase Separation....Pages 455-461
Application of Partition Technology to Particle Electrophoresis....Pages 463-470
Formation of Beads in Aqueous Two-Phase Systems....Pages 471-472
Comparison of Cell and Macromolecular Partition in PEG 8000/Dextran T500, Aquaphase PPT or Reppal PES 200 Phase Systems....Pages 473-476
Some Rheological Properties of Perfluorocarbon Emulsions and Their Potential use in Countercurrent Chromatography Distribution and Chromatography Separation Systems....Pages 477-478
Back Matter....Pages 479-504