RNA Processing Part A: General Methods

Content: 
Contributors to volume 180
Pages ix-xi

Preface
Page xiii
James E. Dahlberg, John N. Abelson

Volumes in series
Pages xv-xxix

[1] Isolation of RNA Original Research Article
Pages 3-13
Shelby L. Berger, John M. Chirgwin

[2] Purification of RNA molecules gel techniques Original Research Article
Pages 14-25
Toshimichi Ikemura

[3] Purifying RNA by column chromatography Original Research Article
Pages 25-41
N.Kyle Tanner

[4] Synthesis of long, capped transcripts in Vitro by SP6 and T7 RNA polymerases Original Research Article
Pages 42-50
Joel K. Yisraeli, Doug A. Melton

[5] Synthesis of small RNAs using T7 RNA polymerase Original Research Article
Pages 51-62
John F. Milligan, Olke C. Uhlenbeck

[6] In vitro synthesis of end-mature, intron-containing transfer RNAs Original Research Article
Pages 63-69
Vicente M. Reyes, John N. Abelson

[7] Preparation of precursors to mRNA from mammalian cell nuclei Original Research Article
Pages 69-82
Selina Chen-Kiang, Daniel J. Lavery

[8] Pulse labeling of heterogeneous nuclear RNA in isolated nuclei Original Research Article
Pages 82-96
Selina Chen-Kiang, Daniel J. Lavery

[9] Isolation and characterization of yeast ribosomal RNA precursors and preribosomes Original Research Article
Pages 96-109
Jacobus Klootwuk, Rudi J. Planta

[10] Preparation of yeast transfer RNA precursors in Vivo Original Research Article
Pages 110-117
Gayle Knapp

[11] Dideoxy sequencing of RNA using reverse transcriptase Original Research Article
Pages 121-130
Chang Shahn, Ellen G. Strauss, James H. Strauss

[12] RNA fingerprinting Original Research Article
Pages 130-154
Andrea D. Branch, Bonnie J. Benenfeld, Hugh D. Robertson

[13] Enzymatic RNA sequencing Original Research Article
Pages 154-163
Y. Kuchino, S. Nishimura

[14] Characterization of cap structures Original Research Article
Pages 164-176
Yasuhiro Furuichi, Aaron J. Shatkin

[15] Isolation and characterization of branched oligonucleotides from RNA Original Research Article
Pages 177-191
J. David Reilly, John C. Wallace, Randa F. Melhem, David W. Kopp, Mary Edmonds

[16] Enzymatic approaches to probing of RNA secondary and tertiary structure Original Research Article
Pages 192-212
Gayle Knapp

[17] A guide for probing native small nuclear RNA and ribonucleoprotein structures Original Research Article
Pages 212-227
Alain Krol, Philippe Carbon

[18] Phylogenetic comparative analysis of RNA secondary structure Original Research Article
Pages 227-239
Bryan D. James, Gary J. Olsen, Norman R. Pace

[19] Mapping the genetic organization of RNA by electron microscopy Original Research Article
Pages 239-261
Louise T. Chow, Thomas R. Broker

[20] Computer prediction of RNA structure Original Research Article
Pages 262-288
Michael Zuker

[21] RNA pseudoknot: Structure, detection, and prediction Original Research Article
Pages 289-303
C.W.A. Pleij, L. Bosch

[22] absorbance melting curves of RNA Original Research Article
Pages 304-325
Joseph D. Puglisi, Ignacio Tinoco Jr.

[23] Nuclease digestion: A method for mapping introns Original Research Article
Pages 325-334
Shelby L. Berger

[24] Characterization of RNA molecules by S1 nuclease analysis Original Research Article
Pages 334-347
Arnold J. Berk

[25] Primer extension analysis of RNA Original Research Article
Pages 347-369
William R. Boorstein, Elizabeth A. Craig

[26] Modification interference analysis of reactions using RNA substrates Original Research Article
Pages 369-379
Laura Conway, Marvin Wickens

[27] Photoaffinity cross-linking methods for studying RNA-protein interactions Original Research Article
Pages 383-409
Michelle M. Hanna

[28] Ultraviolet-induced cross-linking of rna to proteins in vivo Original Research Article
Pages 410-418
SerafГ­n PiЕ„ol-Roma, Stephen A. Adam, Yang Do Choi, Gideon Dreyfuss

[29] Analysis of ultraviolet-induced RNA-RNA cross-links: A means for probing RNA structure-function relationships Original Research Article
Pages 418-442
Andrea D. Branch, Bonnie J. Benenfeld, Cynthia P. Paul, Hugh D. Robertson

[30] analysis of splicing complexes and small nuclear ribonucleoprotein particles by native gel electrophoresis Original Research Article
Pages 442-453
Maria M. Konarska

[31] Determination of RNA-protein and RNA-ribonucleoprotein interactions by nuclease probing Original Research Article
Pages 454-468
Katherine A. Parker, Joan A. Steitz

[32] Immunoprecipitation of ribonucleoproteins using autoantibodies Original Research Article
Pages 468-481
Joan A. Steitz

[33] Electron microscopy of ribonucleoprotein complexes on nascent RNA using miller chromatin spreading method Original Research Article
Pages 481-509
Yvonne N. Osheim, Ann L. Beyer

[34] Genetic Methods for identification and characterization of RNA-RNA and RNA-protein interactions Original Research Article
Pages 510-517
Roy Parker

[35] Compilation of small nuclear RNA sequences Original Research Article
Pages 521-532
Ram Reddy

[36] Sequences and classification of group I and group II introns Original Research Article
Pages 533-545
John M. Burke

[37] Compilation of self-cleaving sequences from plant virus satellite RNAs and other sources Original Research Article
Pages 546-558
George Bruening

Author index
Pages 559-578

Subject index
Pages 579-600