RNA and Protein Synthesis

Content: 
SELECTED METHODS IN ENZYMOLOGY SERIES, Page ii
Front Matter, Page iii
Copyright, Page iv
List of Contributors, Pages ix-xiii
Foreword, Page xv, SIDNEY P. COLOWICK, NATHAN O. KAPLAN
Preface, Page xvii, KIVIE MOLDAVE
1 - Reversed-Phase Chromatography Systems for Transfer Ribonucleic Acids — Preparatory-Scale Methods, Pages 3-28, A.D. KELMERS, H.O. WEEREN, J.F. WEISS, R.L. PEARSON, M.P. STULBERG, G. DAVID NOVELLI
2 - Miniature Reversed-Phase Chromatography Systems for the Rapid Resolution of Transfer RNA\'s and Ribosomal RNA\'s, Pages 29-32, A.D. KELMERS, D.E. HEATHERLY, B.Z. EGAN
3 - Transfer RNA Isoacceptors in Cultured Chinese Hamster Ovary Cells, Pages 33-43, ARNOLD E. HAMPEL, BRUCE RUEFER
4 - The Use of BD-Cellulose in Separating Transfer RNA\'s, Pages 43-58, I.C. GILLAM, G.M. TENER
5 - Fractionation of Rabbit Liver Methionyl-tRNA Species, Pages 58-65, KALLOL K. BOSE, NANDO K. CHATTERJEE, NABA K. GUPTA
6 - RNase Hydrolysis of Aminoacyl-sRNA to Aminoacyl Adenosine, Pages 66-70, GEORGE ACS, FRITZ LIPMANN
7 - Determination of Adenosine- and Aminoacyl Adenosine-Terminated sRNA Chains by Ion-Exclusion Chromatography, Pages 70-74, RICHARD WOLFENDEN
8 - Enzymic Modification of the C-C-A Terminus of tRNA, Pages 74-83, MATHIAS SPRINZL, HANS STERNBACH
9 - Methods for Determining the Extent of tRNA Aminoacylation in Vivo in Cultured Mammalian Cells, Pages 83-87, IRENE L. ANDRULIS, STUART M. ARFIN
10 - The Chemical Preparation of Acetylaminoacyl-tRNA, Pages 87-90, SARA RAPPOPORT, YEHUDA LAPIDOT
11 - The Synthesis of Oligopeptidyl-tRNA, Pages 90-97, YEHUDA LAPIDOT, SARA RAPPOPORT
12 - Preparation of Aminoacyl-Oligonucleotides and Their Binding to Ribosomes, Pages 98-103, SIDNEY PESTKA
13 - Chemical Modifications of Amino Acids Esterified to tRNA (Other Than Acylations), Pages 103-106, S. CHOUSTERMAN, F. CHAPEVILLE
14 - Use of in Vitro32P Labeling in the Sequence Analysis of Nonradioactive tRNAs, Pages 106-157, MELVIN SILBERKLANG, AMANDA M. GILLUM, UTTAM L. RAJBHANDARY
15 - A New Method for Attachment of Fluorescent Probes to tRNA, Pages 158-168, SCOTT A. REINES, LADONNE H. SCHULMAN
16 - Attachment of Cross-Linking Reagents to tRNA for Protein Affinity Labeling Studies, Pages 168-178, ASOK K. SARKAR, LADONNE H. SCHULMAN
17 - Enzymatic Synthesis of Trinucleoside Diphosphates of Known Sequence, Pages 178-180, PHILIP LEDER
18 - Use of Purified Isoacceptor tRNAs for the Study of Codon-Anticodon Recognition in Vitro with Sequenced Natural Messenger RNA, Pages 180-198, EMANUEL GOLDMAN, G. WESLEY HATFIELD
19 - Rabbit Liver tRNA Nucleotidyltransferase, Pages 198-208, MURRAY P. DEUTSCHER
20 - Bacterial tRNA Methyltransferases, Pages 208-221, ROBERT GREENBERG, BERNARD S. DUDOCK
21 - Assay for AA-tRNA Recognition by the EFTu-GTP Complex of Escherichia coli, Pages 221-227, JAMES OFENGAND
22 - Mapping the Structure of Specific Protein-Transfer RNA Complexes by a Tritium Labeling Method, Pages 227-245, PAUL R. SCHIMMEL, HUBERT J.P. SCHOEMAKER
23 - Aminoacyl-tRNA Synthetase Complex from Rat Liver, Pages 249-255, MURRAY P. DEUTSCHER
24 - Kinetic Techniques for the Investigation of Amino Acid : tRNA Ligases (Aminoacyl-tRNA Synthetases, Amino Acid Activating Enzymes), Pages 256-274, ELIZABETH ANN EIGNER, ROBERT B. LOFTFIELD
25 - Kinetics of Aminoacyl-tRNA Synthetases Catalyzed ATP-PPi Exchange, Pages 275-282, DANIEL V. SANTI, ROBERT W. WEBSTER JR., W.W. CLELAND
26 - Applications of Kinetic Methods to Aminoacyl-tRNA Synthetases, Pages 282-297, CHRISTIAN F. MIDELFOHT, ALAN H. MEHLER
27 - Purification of Protein Synthesis Initiation Factors IF-1, IF-2, and IF-3 from Escherichia coli, Pages 301-309, JOHN W.B. HERSHEY, JOAN YANOV, JOHN L. FAKUNDING
28 - Protein Synthesis Initiation Factors from Rabbit Reticulocytes: Purification, Characterization, and Radiochemical Labeling, Pages 309-329, ROB BENNE, MARIANNE L. BROWN-LUEDI, JOHN W.B. HERSHEY
29 - Initiation Factors for Protein Synthesis from Wheat Germ, Pages 329-341, BENJAMIN V. TREADWELL, LJUBICA MAUSER, WILLIAM G. ROBINSON
30 - Assay of the Binding of the Ternary Complex Met-tRNAf·eIF-2·GTP to the 40 S Ribosomal Subunit by Sucrose Gradient and CsCl Gradient Analysis, Pages 342-347, EDGAR C. HENSHAW
31 - Prokaryotic Ribosome Binding Sites, Pages 347-358, JOAN ARGETSINGER STEITZ
32 - Characterization of Translational Initiation Regions from Eukaryotic Messenger RNAs, Pages 358-373, MARILYN KOZAK, AARON J. SHATKIN
33 - Determination of the Number of Ribosomal Binding Sites on the RNAs of Eukaryotic Viruses, Pages 373-380, LYDA NEELEMAN, LOUS VAN VLOTEN-DOTING
34 - Methods for Analyzing Messenger Discrimination in Eukaryotic Initiation Factors, Pages 381-385, CLAIRE H. BIRGE, FRED GOLINI, ROBERT E. THACH
35 - Isolation of Protein Kinases from Reticulocytes and Phosphorylation of Initiation Factors, Pages 386-401, GARY M. HATHAWAY, TINA S. LUNDAK, STANLEY M. TAHARA, JOLINDA A. TRAUGH
36 - Isolation of the Protein Synthesis Elongation Factors EF-Tu, EF-Ts, and EF-G from Escherichia coli, Pages 405-418, PETER WURMBACH, KNUD H. NIERHAUS
37 - Preparation of Homogeneous Escherichia coli Translocation Factor G, Pages 418-421, PHILIP LEDER
38 - The Binding of Escherichia coli Elongation Factor G to the Ribosome, Pages 422-424, JAMES W. BODLEY, HERBERT WEISSBACH, NATHAN BROT
39 - GTP-Dependent Binding of Aminoacyl-tRNA to Escherichia coli Ribosomes-, Pages 425-435, JOANNE M. RAVEL, ROSEANN L. SHOREY
40 - Polypeptide Chain Elongation Factors from Pig Liver, Pages 435-454, KENTARO IWASAKI, YOSHITO KAZIRO
41 - Separation of Cytoplasmic and Mitochondrial Elongation Factors from Yeast, Pages 454-463, DIETMAR RICHTER, FRITZ LIPMANN
42 - Soluble Protein Factors and Ribosomal Subunits from Yeast. Interactions with Aminoacyl-tRNA, Pages 463-470, A. TORAÑO, A. SANDOVAL, C.F. HEREDIA
43 - Separation and Characterization of Yeast Elongation Factors, Pages 471-480, LAWRENCE SKOGERSON
44 - Release Factors: in Vitro Assay and Purification, Pages 483-491, C.T. CASKEY, E. SCOLNICK, R. TOMPKINS, G. MILMAN, J. GOLDSTEIN
45 - Mammalian Release Factor; in Vitro Assay and Purification, Pages 491-501, C.T. CASKEY, A.L. BEAUDET, W.P. TATE
46 - Sedimentation Velocity Analysis in Accelerating Gradients, Pages 505-511, RAYMOND KAEMPFER, MATTHEW MESELSON
47 - Preparation of Ribosomal Subunits by Large-Scale Zonal Centrifugation, Pages 512-517, PAUL S. SYPHERD, JOHN W. WIREMAN
48 - A Gel Electrophoretic Separation of Bacterial Ribosomal Subunits with and without Protein S1, Pages 517-521, ALBERT E. DAHLBERG
49 - Reconstitution of Ribosomes from Subribosomal Components, Pages 521-538, P. TRAUB, S. MIZUSHIMA, C.V. LOWRY, M. NOMURA
50 - Total Reconstitution of 50 S Subunits from Escherichia coli Ribosomes, Pages 538-544, KNUD H. NIERHAUS, FERDINAND DOHME
51 - Purification of Ribosomal Proteins from Escherichia coli, Pages 545-554, C.G. KURLAND, S.J.S. HARDY, G. MORA
52 - Purification of Ribosomal Proteins from Escherichia coli under Nondenaturing Conditions, Pages 554-575, JAN DIJK, JENNY LITTLECHILD
53 - Two-Dimensional Polyacrylamide Gel Electrophoresis for Separation of Ribosomal Proteins, Pages 576-584, H.G. WITTMANN
54 - A Modified Two-Dimensional Gel System for the Separation and Radioautography of Microgram Amounts of Ribosomal Proteins, Pages 584-597, G.A. HOWARD, R.R. TRAUT
55 - Analytical Methods for Ribosomal Proteins of Rat Liver 40 S and 60 S Subunits by “Three-Dimensional” Acrylamide Gel Electrophoresis, Pages 598-610, KIKUO OGATA, KAZUO TERAO
56 - Cross-Linking of Ribosomes Using 2-Iminothiolane (Methyl 4-Mercaptobutyrimidate) and Identification of Cross-Linked Proteins by Diagonal Polyacrylamide/Sodium Dodecyl Sulfate Gel Electrophoresis, Pages 610-626, JAMES W. KENNY, JOHN M. LAMBERT, ROBERT R. TRAUT
57 - Photoaffinity Labeling of Ribosomes, Pages 627-646, B.S. COOPERMAN, P.G. GRANT, R.A. GOLDMAN, M.A. LUDDY, A. MINNELLA, A.W. NICHOLSON, W.A. STRYCHARZ
58 - Radioactive Chemical Labeling of Ribosomal Proteins and Translational Factors in Vitro, Pages 646-659, CLAUDIO GUALERZI, CYNTHIA L. PON
59 - Peptidyl-Puromycin Synthesis on Polyribosomes from Escherichia coli, Pages 660-669, SIDNEY PESTKA
60 - Separation of Large Quantities of Eukaryotic Ribosomal Subunits by Zonal Ultracentrifugation, Pages 669-682, CORINNE C. SHERTON, RALPH F. DI CAMELLI, IRA G. WOOL
61 - Two-Dimensional Polyacrylamide Gel Electrophoresis of Eukaryotic Ribosomal Proteins, Pages 683-703, CORINNE C. SHERTON, IRA G. WOOL
62 - Isotopic Labeling and Analysis of Phosphoproteins from Mammalian Ribosomes, Pages 703-731, LAWRENCE BITTE, DAVID KABAT
63 - Phosphorylation of Ribosomal Proteins: Preparation of Rat Liver Ribosomal Protein Kinases. Assay of the Phosphorylation of Ribosomal Subunit Proteins. Assay of the Function of Phosphorylated Ribosomes, Pages 731-742, CHARLES EIL, IRA G. WOOL
64 - Protein-RNA Interactions in the Bacterial Ribosome, Pages 743-775, ROBERT A. ZIMMERMANN
65 - The Use of Membrane Filtration to Determine Apparent Association Constants for Ribosomal Protein-RNA Complex Formation, Pages 775-783, JEAN SCHWARZBAUER, GARY R. CRAVEN
66 - Preparation and Analysis of Mitochondrial Ribosomes, Pages 783-795, ALAN M. LAMBOWITZ
Index, Pages 797-812