Rheumatoid Arthritis: Methods and Protocols

Front Matter ....Pages i-ix
Front Matter ....Pages 1-1
 Collagen-Induced Arthritis Models  (Maya Miyoshi, Shuang Liu)....Pages 3-7
 Human Xenograft Model  (Shuang Liu)....Pages 9-15
 Long-Term Constant Subcutaneous Drug Administration  (Shuang Liu, Maya Miyoshi)....Pages 17-22
 Clinical Scoring of Disease Activity in Animal Models  (Maya Miyoshi, Shuang Liu)....Pages 23-28
 Histological Analysis of Arthritic Joints  (Takeshi Kiyoi)....Pages 29-39
 Preparation of Joint Extracts  (Shuang Liu, Erika Takemasa)....Pages 41-45
Front Matter ....Pages 47-47
 Production of Immunizing Antigen Proteoliposome Using Cell-Free Protein Synthesis System  (Wei Zhou, Hiroyuki Takeda)....Pages 49-67
 Reconstruction of Protein/Liposome Complex  (Yasuyuki Suzuki)....Pages 69-77
 Production of Neutralizing Antibody  (Erika Takemasa, Shuang Liu, Hitoshi Hasegawa)....Pages 79-92
 Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen  (Hiroyuki Takeda)....Pages 93-112
 Generation of Specific Aptamers  (Shuang Liu, Yasuyuki Suzuki, Makoto Inui)....Pages 113-121
 Production of Lentiviral Particles  (Shuang Liu)....Pages 123-128
 RNA Interference Ex Vivo  (Shuang Liu)....Pages 129-135
 Lentiviral-Mediated Systemic RNA Interference In Vivo  (Shuang Liu)....Pages 137-144
 Mesenchymal Stem Cell Engineering  (Shuang Liu)....Pages 145-150
Front Matter ....Pages 151-151
 Screening of Ca2+ Influx in Lymphocytes  (Erika Takemasa, Shuang Liu)....Pages 153-159
 Single-Cell Ca2+ Imaging  (Shuang Liu)....Pages 161-168
 Electrophysiological Methods to Measure Ca2+ Current  (Shuang Liu, Miyuki Kuno)....Pages 169-176
 The Functional Assessment of T cells  (Saho Maruyama)....Pages 177-199
 Release of Antibodies and Cytokines from B Cells  (Shuang Liu)....Pages 201-207
 Evaluation of Autoreactive Responses  (Shuang Liu)....Pages 209-213
 Bone Resorption Activity in Mature Osteoclasts  (Takeshi Kiyoi)....Pages 215-222
 Animal Models of Vasculitis  (M. Mogi, Shuang Liu)....Pages 223-232
Front Matter ....Pages 233-233
 Design an Intervention Study  (Hitoshi Hasegawa)....Pages 235-241
 Assessment of Disease Activity, Structural Damage, and Function in Rheumatoid Arthritis  (Hitoshi Hasegawa)....Pages 243-250
 Correction to : Production of Lentiviral Particles  (Shuang Liu)....Pages E1-E1
Back Matter ....Pages 251-253