Retroviral Proteases: Control of maturation and morphogenesis

Front Matter....Pages i-xi
Introduction....Pages 1-3
Characterisation and Inhibition of the Retroviral HIV-Protease....Pages 5-8
The HIV-1 Aspartyl Protease: Maturation and Substrate Specificity....Pages 9-17
Cleavage of RT/RNase H by HIV-1 Protease and Analysis of Substrate Cleavage Sites in vitro....Pages 19-29
Retroviral Protease: Substrate Specificity and Inhibitors....Pages 31-39
HIV pol Expression via a Ribosomal Frameshift....Pages 41-53
Functional Characterisation of HIV-1 gag-pol Fusion Protein....Pages 55-62
Biosynthesis and Processing of the gag and pol Polyproteins of Human Immunodeficiency Virus Type 1 in Escherichia coli....Pages 63-71
Dimerisation of the HIV-1 Protease: Preliminary Analysis Using Gel Permeation Chromatography....Pages 73-78
Three-dimensional Structure and Evolution of HIV-1 Protease....Pages 79-91
X-Ray Analysis of HIV-1 Protease and Its Complexes with Inhibitors....Pages 93-106
Expression of HIV-1 gag and pol Gene Products using Recombinant Baculoviruses....Pages 107-115
Morphogenesis of Retroviruses: A Complex Role for the Matrix (MA) Protein in Assembly, Stability and Intracellular Transport of Capsid Proteins....Pages 117-129
The Action of Retroviral Protease in Various Phases of Virus Replication....Pages 131-139
Terminal Stages of Retrovirus Morphogenesis....Pages 141-148
Analysis of the Assembly of the HIV Core by Electron Microscope Tomography....Pages 149-157
Morphogenesis, Maturation and Fine Structure of Lentiviruses....Pages 159-180
Back Matter....Pages 181-183