Regulators of G-Protein Signaling, Part A

Content: 
Editors-In-Chief
Page ii

Contributors to Volume 389
Pages ix-xii

Preface
Pages xiii-xv

Methods In Enzymology
Pages xvii-xxxix

Quantitative Real-Time Polymerase Chain Reaction Measurement of Regulators of G-Protein Signaling mRNA Levels in Mouse Tissues Original Research Article
Pages 3-15
Deborah M Kurrasch, Jie Huang, Thomas M Wilkie, Joyce J Repa

Regulation of Chemokine-Induced Lymphocyte Migration by RGS Proteins Original Research Article
Pages 15-32
Chantal Moratz, Kathleen Harrison, John H. Kehrl

Role of Palmitoylation in RGS Protein Function Original Research Article
Pages 33-55
Teresa L.Z. Jones

Fluorescence-Based Assays for RGS Box Function Original Research Article
Pages 56-71
Francis S. Willard, Randall J. Kimple, Adam J. Kimple, Christopher A. Johnston, David P. Siderovski

Application of RGS Box Proteins to Evaluate G-Protein Selectivity in Receptor-Promoted Signaling Original Research Article
Pages 71-88
Melinda D Hains, David P Siderovski, T.Kendall Harden

Allosteric Regulation of GAP Activity by Phospholipids in Regulators of G-Protein Signaling Original Research Article
Pages 89-1005
Yaping Tu, Thomas M. Wilkie

Assays of RGS Protein Modulation by Phosphatidylinositides and Calmodulin Original Research Article
Pages 105-118
Masaru Ishii, Yoshihisa Kurachi

Analyses of RGS Protein Control of Agonist-Evoked Ca2+ Signaling Original Research Article
Pages 119-130
Xiang Luo, Wooin Ahn, Shmuel Muallem, Weizhong Zeng

Measuring the Modulatory Effects of RGS Proteins on GIRK Channels Original Research Article
Pages 131-154
Craig A Doupnik, Cristina JaГ©n, Qingli Zhang

Assays for G-Protein-Coupled Receptor Signaling Using RGS-Insensitive GО± Subunits Original Research Article
Pages 155-169
Mary J. Clark, John R. Traynor

Use of RGS-Insensitive GО± Subunits to Study Endogenous RGS Protein Action on G-Protein Modulation of N-Type Calcium Channels in Sympathetic Neurons Original Research Article
Pages 170-189
Stephen R Ikeda, Seong-Woo Jeong

Endogenous RGS Proteins Regulate Presynaptic and Postsynaptic Function: Functional Expression of RGS-Insensitive GО± Subunits in Central Nervous System Neurons Original Research Article
Pages 190-204
Huanmian Chen, Michael A Clark, Nevin A Lambert

In Situ Hybridization Analysis of RGS mRNA Regulation and Behavioral Phenotyping of RGS Mutant Mice Original Research Article
Pages 205-229
Stephen J Gold, Venetia Zachariou

RGS-Insensitive G-Protein Mutations to Study the Role of Endogenous RGS Proteins Original Research Article
Pages 229-243
Ying Fu, Huailing Zhong, Masakatsu Nanamori, Richard M Mortensen, Xinyan Huang, Kengli Lan, Richard R Neubig

Ribozyme- and siRNA-Mediated Suppression of RGS-Containing RhoGEF Proteins Original Research Article
Pages 244-265
Qin Wang, Min Liu, Tohru Kozasa, Jeffrey D Rothestein, Paul C Sternweis, Richard R Neubig

Structure-Based Design, Synthesis, and Activity of Peptide Inhibitors of RGS4 GAP Activity Original Research Article
Pages 266-277
Yafei Jin, Huailing Zhong, John R. Omnaas, Richard R. Neubig, Henry I. Mosberg

Yeast-Based Screening for Inhibitors of RGS Proteins Original Research Article
Pages 277-301
Kathleen H. Young, Yuren Wang, Corey Bender, Seena Ajit, Fernando Ramirez, Adam Gilbert, Bart W. Nieuwenhuijsen

Genetic Analysis of RGS Protein Function in Caenorhabditis elegans Original Research Article
Pages 305-320
Daniel L Chase, Michael R Koelle

Purification and In Vitro Functional Analysis of the Arabidopsis thaliana Regulator of G-Protein Signaling-1 Original Research Article
Pages 320-338
Francis S Willard, David P Siderovski

AtRGS1 Function in Arabidopsis thaliana Original Research Article
Pages 338-350
Jin-Gui Chen, Alan M Jones

Identification and Functional Analysis of the Drosophila Gene loco Original Research Article
Pages 350-363
Sebastian Granderath, Christian Klämbt

Analysis of the Roles of Pins and Heterotrimeric G Proteins in Asymmetric Division of Drosophila Neuroblasts Original Research Article
Pages 364-382
Fengwei Yu

Mathematical Modeling of RGS and G-Protein Regulation in Yeast Original Research Article
Pages 383-398
Necmettin Yildirim, Nan Hao, Henrik G. Dohlman, Timothy C. Elston

Identification of Yeast Pheromone Pathway Modulators by High-Throughput Agonist Response Profiling of a Yeast Gene Knockout Strain Collection Original Research Article
Pages 399-409
Scott A. Chasse, Henrik G. Dohlman

Author Index
Pages 411-426

Subject Index
Pages 427-434