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Refractory organic substances (ROS) are an essential part of
the biogeochemical carbon cycle. Wherever there is life on
earth, there will also be ROS in the form of poorly
biodegradable leftovers of organisms and as a source for new
life. Furthermore, it is now beyond doubt that ROS are closely
related to the carbon intensity identified as one of the
driving forces in the dynamics of green house gas emission,
such that ROS play a key role in sustainable development.
Refractory Organic Substances in the Environment
provides the results of six years of top-priority research,
funded by the Deutsche Forschungsgemeinschaft (DFG). This
research program investigated the structure and function of
ROS in different parts of the environment, from a chemical,
physical, biological, and soil scientific point of view. It
included the first systematic study of a set of reference
samples from Central Europe, originating from a bog lake,
soil seepage water, groundwater, and from the wastewaters of
a brown coal processing plant and a secondary effluent. Thus,
this work not only highlights the structural features
obtained from the application of advanced analytical tools,
but also the function in anthropogenically influenced aquatic
systems and soils. Of special interest to students and
researchers in life sciences.
Content:
Chapter 1 Setting the Scene: The Relevance of Reference
Materials ? Isolation and General Characterization (pages
1–38): Dr. G. Abbt?Braun and Prof. Dr. F. H. Frimmel
Chapter 1 Setting the Scene: Element Determination and its
Quality Control in Fractions of Refractory Organic Substances
and the Corresponding Original Water Samples (pages 39–53):
Prof. Dr. K. G. Heumann, Dr. G. Abbt?Braun, K. Behrens, Dr. P.
Burba, Prof. Dr. F. H. Frimmel, Dr. B. Jakubowski, Professor A.
Knochel, Dr. J. Mielcke, Dr. G. Radlinger, Dr. G. Marx and Dr.
J. Vogl
Chapter 2 Structural Investigations: Heavy Metal and Halogen
Interactions with Fractions of Refractory Organic Substances
Separated by Size?exclusion Chromatography (pages 55–72): Prof.
Dr. K. G. Heumann, Dr. G. Marx, Dr. G. Radlinger and Dr. J.
Vogl
Chapter 2 Structural Investigations: Characterization of
Refractory Organic Substances and their Metal Species by
Combined Analytical Procedures (pages 73–88): Dr. P. Burba, Dr.
B. Jakubowski and J. Van den Bergh
Chapter 2 Structural Investigations: Application of Nuclear
Magnetic Resonance Spectroscopy to Structural Investigations of
Refractory Organic Substances – Principles and Definitions
(pages 89–95): Dr. J. Lambert and Dr. U. Lankes
Chapter 2 Structural Investigations: Structural
Characterization of Refractory Organic Substances by
Solid?state High?resolution 13C and 15N Nuclear Magnetic
Resonance (pages 96–114): Dr. U. Lankes and Professor H.?D.
Ludemann
Chapter 2 Structural Investigations: Quantification of
Substructures of Refractory Organic Substances by Means of
Nuclear Magnetic Resonance (pages 115–128): Dr. S. Haiber, H.
Herzog, Professor J. Buddrus, Dr. P. Burba and Dr. J.
Lambert
Chapter 2 Structural Investigations: Nuclear Magnetic Resonance
Spectroscopy Investigations of Silylated Refractory Organic
Substances (pages 129–145): Dr. N. Hertkorn, Dr. A. Gunzl, Dr.
D. Freitag and Professor A. Kettrup
Chapter 2 Structural Investigations: Isotopic Evidence for the
Origin and Formation of Refractory Organic Substances (pages
146–162): Dr. G. Gleixner, Dr. O. Kracht, Dr. H.?L. Schmidt and
Dr. E.?D. Schulze
Chapter 2 Structural Investigations: Analytical Pyrolysis of
Humic Substances and Dissolved Organic Matter in Water (pages
163–187): Professor H.?R. Schulten, Professor P. Leinweber and
Dr. G. Jandl
Chapter 2 Structural Investigations: Characterization of
Refractory Organic Substances by HPLC/MS (pages 188–199):
Professor M. Spiteller, Dr. U. Klaus and Dr. T. Pfeifer
Chapter 2 Structural Investigations: UV?Visible Spectroscopy
and the Potential of Fluorescent Probes (pages 200–214):
Professor H. Langhals
Chapter 2 Structural Investigations: Stationary and
Time?resolved Fluorescence for Refractory Organic Substances
Characterization (pages 215–231): Dr. M. U. Kumke and Prof. Dr.
F. H. Frimmel
Chapter 2 Structural Investigations: Structural
Characterization of Refractory Organic Substances by
Pyrolysis?GC/FTIR (pages 232–238): Dr. A. N. Davies, Dr. W.
Hill and Dipl.?Ing. R. Kuckuk
Chapter 2 Structural Investigations: X?Ray Microscopy Studies
of Refractory Organic Substances (pages 239–248): Dr. J.
Thieme, Dr. C. Schmidt, Dr. G. Abbt?Braun, C. Specht and Prof.
Dr. F. H. Frimmel
Chapter 2 Structural Investigations: Fractionation of
Refractory Organic Substances by Electrophoresis (pages
249–263): Dr. H. H. Ruttinger and Dr. R. Dunkel
Chapter 2 Structural Investigations: Occurrence of Amino Acids,
Carbohydrates, and Low?Molecular?weight Organic Acids in
Refractory Organic Substances (pages 264–281): Dr. J. B.
Jahnel, T. Brinkmann, Dr. G. Abbt?Braun and Prof. Dr. F. H.
Frimmel
Chapter 2 Structural Investigations: Serological
Characterization of Refractory Organic Substances by Serotyping
(pages 282–301): Dr. R. Muller?Starck, M. Kania, Dr. A.
Dankwardt and Professor B. Hock
Chapter 2 Structural Investigations: Chemical and Spectroscopic
Data of the Reference Samples – Comparison and Evaluation
(pages 302–309): Dr. G. Abbt?Braun, Dr. U. Lankes, Dr. J. B.
Jahnel, Dr. J. Lambert, Professor H.?D. Ludemann and Prof. Dr.
F. H. Frimmel
Chapter 3 Biochemical and Biological Characterization:
Formation, Utilization, and Transformation of Some Refractory
Organic Substances by Aquatic Microorganisms (pages 311–320):
Professor Z. Filip and Dr. H. Claus
Chapter 3 Biochemical and Biological Characterization: Effect
of Microorganisms on the Formation and Transformation of Iodine
Species of Refractory Organic Substances (pages 321–329):
Professor K. G. Heumann, Dr. G. Radlinger, Dr. H. Claus,
Professor Z. Filip, M. Erbes, I. Heiber and PD Dr. U.
Obst
Chapter 3 Biochemical and Biological Characterization: The
Influence of Refractory Organic Substances on Bacterial
Colonization and Diversity Patterns (pages 330–345): Dr. T.
Koch, S. Honschopp, O. Janssen?Weets and Professor A.
Nehrkorn
Chapter 3 Biochemical and Biological Characterization:
Influence of Refractory Organic Substances on Enzyme Activity
in?vivo and DNA Damage of Aquatic Microorganisms (pages
346–360): I. Heiber, Dr. M. Wiegand?Rosinus, M. Erbes and PD
Dr. U. Obst
Chapter 3 Biochemical and Biological Characterization: Effects
of Dissolved Organic Matter on the Bioconcentration of Organic
Contaminants and on Reproduction in Aquatic Invertebrates
(pages 361–381): Dr. M. Haitzer, Dr. S. Hoss, Professor W.
Traunspurger, Professor J. Kukkonen, Dr. B. K. Burnison and
Professor C. E. W. Steinberg
Chapter 4 Molecular Interactions: Sorption of Dissolved Organic
Matter on Soil Particles and its Dependence on their
Surface?charge Properties (pages 383–393): Professor W. R.
Fischer and Dr. F. Buttchereit
Chapter 4 Molecular Interactions: Dissolved Organic Carbon in
Seepage Water – Production and Transformation During Soil
Passage (pages 394–410): PD Dr. B. Ludwig, Dr. B. Heil, H.
Flessa and Professor F. Beese
Chapter 4 Molecular Interactions: Refractory Organic Substances
in Aggregated Forest Soils – Retention versus Translocation
(pages 411–434): Dr. K. Kaiser, PD Dr. G. Guggenberger,
Professor M. Kaupenjohann and Professor W. Zech
Chapter 4 Molecular Interactions: Refractory Organic Substances
Derived from Organic Amendments in Soil – Formation,
Translocation, and Interaction with Xenobiotics (pages
435–445): Dr. Th. Putz, Professor F. Fuhr and S. Brandt
Chapter 4e Molecular Interactions: Analysis of the Binding of
Amitrole and Anilazine to Aquatic and Terrestrial Refractory
Organic Substances (pages 446–474): Professor M. Spiteller, Dr.
U. Klaus and Dr. T. Pfeifer
Chapter 4 Molecular Interactions: Sorption and Chemical
Reactions of Polycyclic Aromatic Hydrocarbons with Dissolved
Refractory Organic Substances and Related Model Polymers (pages
475–515): Professor F.?D. Kopinke, Dr. A. Georgi, Dr. K.
Mackenzie and Dr. M. U. Kumke
Chapter 4 Molecular Interactions: Investigation of the
Interactions between Polycyclic Aromatic Compounds and
Refractory Organic Substances with Stationary and Time?Resolved
Fluorescence and Absorption Spectroscopy (pages 516–534):
Professor H.?G. Lohmannsroben, Dr. U. Schultze and Dr. T.
Skrivanek