Recombinant Protein Expression in Mammalian Cells: Methods and Protocols

Front Matter ....Pages i-xi
 Transient Gene Expression in Suspension HEK293-EBNA1 Cells  (Denis L’Abbé, Louis Bisson, Christian Gervais, Eric Grazzini, Yves Durocher)....Pages 1-16
 Transient Expression of Recombinant Membrane-eGFP Fusion Proteins in HEK293 Cells  (Joanna Pieprzyk, Samuel Pazicky, Christian Löw)....Pages 17-31
 PEI-Mediated Transient Gene Expression in CHO Cells  (Yashas Rajendra)....Pages 33-42
 Stable Expression by Lentiviral Transduction of Cells  (Natascha Gödecke, Hansjörg Hauser, Dagmar Wirth)....Pages 43-55
 Inducible Protein Production in 293 Cells Using the piggyBac Transposon System  (Iacovos P. Michael, Andras Nagy)....Pages 57-68
 Recombinant CHO Cell Pool Generation Using piggyBac Transposon System  (Sowmya Balasubramanian)....Pages 69-78
 Genome Engineering of Hybridomas to Generate Stable Cell Lines for Antibody Expression  (Cristina Parola, Derek M. Mason, Andreas Zingg, Daniel Neumeier, Sai T. Reddy)....Pages 79-111
 Protein Expression via Transient Transfection of Mammalian Cells in a WAVE Bioreactor  (David I. Fisher)....Pages 113-121
 CHO and HEK293 Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors  (David L. Hacker, Laurence Durrer, Soraya Quinche)....Pages 123-131
 Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary (CHO) Cells in Suspension  (Dominique T. Monteil, Jeffrey Kuan)....Pages 133-145
 Continuous and Integrated Expression and Purification of Recombinant Antibodies  (Sebastian Vogg, Moritz Kai Florian Wolf, Massimo Morbidelli)....Pages 147-178
 High Throughput Transfection of HEK293 Cells for Transient Protein Production  (Tia A. Arena, Peter D. Harms, Athena W. Wong)....Pages 179-187
 Microfluidic Transfection for High-Throughput Mammalian Protein Expression  (Kristina Woodruff, Sebastian J. Maerkl)....Pages 189-208
 Genome-Wide High-Throughput RNAi Screening for Identification of Genes Involved in Protein Production  (Sarah Inwood, Michael J. Betenbaugh, Madhu Lal, Joseph Shiloach)....Pages 209-219
 Targeting miRNAs with CRISPR/Cas9 to Improve Recombinant Protein Production of CHO Cells  (Kevin Kellner, Ankur Solanki, Thomas Amann, Nga Lao, Niall Barron)....Pages 221-235
 Application of the CRISPR/Cas9 Gene Editing Method for Modulating Antibody Fucosylation in CHO Cells  (Qiong Wang, Cheng-Yu Chung, Julian N. Rosenberg, Geng Yu, Michael J. Betenbaugh)....Pages 237-257
 Scalable Production and Purification of Adeno-Associated Viral Vectors (AAV)  (Daniel Blessing, Nicole Déglon, Bernard L. Schneider)....Pages 259-274
 Considerations in the Use of Codon Optimization for Recombinant Protein Expression  (Vincent P. Mauro, Stephen A. Chappell)....Pages 275-288
 Versatile Cell-Free Protein Synthesis Systems Based on Chinese Hamster Ovary Cells  (Lena Thoring, Stefan Kubick)....Pages 289-308
Back Matter ....Pages 309-311