Receptor Molecular Biology

Content: 
Contributors to volume 25
Pages ix-xii

Preface
Page xiii
Stuart C. Sealfon

Methods in neurosciences
Pages xv-xvi

[1] Approaches to receptor cloning Original Research Article
Pages 3-8
Stuart C. Sealfon

[2] Purification of receptors Original Research Article
Pages 9-43
David I. Schuster, Randall B. Murphy

[3] Protein and peptide microsequencing: Applications in neuroscience and receptor research Original Research Article
Pages 44-66
Michael J. Walsh

[4] Xenopus oocytes: A system for expression cloning and structure-function studies of ion channels and receptors Original Research Article
Pages 67-89
Roger D. Zühlke, Hui-Juan Zhang, Rolf H. Joho

[5] Cloning of G protein-coupled opioid receptors using degenerate PCR and low-stringency homology screening Original Research Article
Pages 90-104
David K. Grandy, Qun-Yong Zhou, Claudia Bouvier, Carmen Saez, James R. Bunzow

[6] Hybrid arrest screening in oocytes Original Research Article
Pages 105-125
Manami Tsutsumi, Boaz Gillo

[7] Receptor cloning: High-throughput sequencing of cDNA tags for identification of novel genes Original Research Article
Pages 126-141
Ewen F. Kirkness, J. Craig Venter

[8] Ligand binding and second-messenger assays for cloned Gq/G11-coupled neuropeptide receptors: The GnRH receptor Original Research Article
Pages 145-162
R.P. Millar, J. Davidson, I. Wakefield, C. Flanagan

[9] Receptor expression in mammalian cells Original Research Article
Pages 163-174
Rachael L. Neve, Kim A. Neve

[10] Baculovirus expression of receptors and channels Original Research Article
Pages 175-200
Michael Cascio

[11] Real-time measurements of receptor activity: Applications of microphysiometric techniques to receptor biology Original Research Article
Pages 201-224
John A. Salon, John C. Owicki

[12] Regulation of Na+-H+ exchange by G protein-coupled receptors Original Research Article
Pages 225-241
Kim A. Neve, Mary P. Rosser, Diane L. Barber

[13] Receptor-activated tyrosine phosphatases: Activity assays and molecular cloning Original Research Article
Pages 242-260
Philip J.S. Stork, Anita Misra-Press, Ming-Gui Pan

[14] Site-directed mutagenesis Original Research Article
Pages 263-277
Tung Ming Fong, Mari R. Candelore, Catherine D. Strader

[15] Receptor chimers Original Research Article
Pages 278-301
Sankuratri Suryanarayana, Brian K. Kobilka

[16] Mapping G protein coupling domains by site-specific peptides Original Research Article
Pages 302-321
Dieter Palm, Gerald Münch, Daria Malek

[17] Synthesis and expression of synthetic genes: Applications to structure-function studies of receptors Original Research Article
Pages 322-344
Cynthia J.L. Carruthers, Thomas P. Sakmar

[18] Use of receptors expressed in Escherichia coli to study autoimmunity against G protein-coupled membrane proteins Original Research Article
Pages 345-365
Johan Hoebeke, Jean-Gérard Guillet, A. Donny Strosberg

[19] Integrated methods for the construction of three-dimensional models and computational probing of structure-function relations in G protein-coupled receptors Original Research Article
Pages 366-428
Juan A. Ballesteros, Harel Weinstein

[20] Anti-fusion protein antibodies specific for receptor subtypes Original Research Article
Pages 431-454
Brian J. Ciliax, Craig Heilman, Sharon Edmunds, Steven M. Hersch, Allan I. Levey

[21] Development of antireceptor antibodies using synthetic peptides Original Research Article
Pages 455-469
Marjorie A. Ariano, David R. Sibley

[22] Receptor mRNA measurement by multiplex nuclease protection assay Original Research Article
Pages 470-491
Moshe Jakubowski

[23] Antisense DNA/RNA-based strategies to analysis of signal transduction via G proteins Original Research Article
Pages 492-509
Meiling Shih, Christopher M. Moxham, Craig C. Malbon

Index
Pages 511-519