Rapid Methods and Automation in Microbiology and Immunology

Front Matter....Pages I-XVI
The Evaluation of Vaccines....Pages 1-5
Evaluation of Hepatitis B Vaccine....Pages 6-12
Concepts in the Development of Vaccines Against Genital Herpes Simplex Virus Infections....Pages 13-23
Development of a Live Attenuated Vaccine Against Varicella....Pages 24-29
The Use of DNA Hybridization for the Identification of Pathogenic Bacteria....Pages 30-33
Microbial Diagnosis by Nucleic Acid Sandwich Hybridization....Pages 34-42
Diagnosis of Papillomavirus Infection by Nucleic Acid Hybridization....Pages 43-47
Detection of Epstein-Barr Virus DNA by Nucleic Acid Hybridization....Pages 48-56
Diagnosis of Viroid-Caused Plant Diseases by Nucleic Acid Hybridization....Pages 57-67
DNA Technology Applied to the Detection and Epidemiology of Enteric Pathogens....Pages 68-72
New Developments in Nucleic Acid Hybridization....Pages 73-82
Variability of Antigen Epitopes of Monotypic Viruses....Pages 83-93
Immunoblotting with Monoclonal Antibodies: A Highly Specific System for Detection and Identification of Bacterial Outer Membrane Proteins....Pages 94-102
Immunoblotting and Dot Immunobinding....Pages 103-114
Fluorescence-Activated Cell Sorter and Monoclonal Antibodies: Complementary Tools in Immunodiagnosis and Immunotherapy....Pages 115-122
A Microfilter Assay for the Detection of Antibody-Producing Cells In Vitro....Pages 123-128
A Biotin Avidin Amplified ELISA Using Exclusively Monoclonal Antibodies for the Detection of Respiratory Syncytial Viral Antigens in Nasopharyngeal Secretions....Pages 129-134
Identification and Serotyping of Vibrio cholerae 0:1 by Monoclonal Antibody Based Enzyme-Linked Immunosorbent Assays....Pages 135-140
The Role of Specific Antibodies in Different Immunoglobulin Classes in Rapid Diagnosis of Virus Infections....Pages 141-145
Problems of Rubella Diagnosis by Various IgM Techniques and the Need for Test Combinations....Pages 146-161
The Role of Specific Antibodies of Different Immunoglobulin Classes in the Rapid Diagnosis of Systemic Mycotic Infections....Pages 162-168
The Role of Specific Antibodies in Different Immunoglobulin Classes in the Rapid Diagnosis of Diseases Caused by Treponemes....Pages 169-176
NMR in the Study of Cells and Tissues....Pages 177-183
Rapid Microbial Identification by Circular Intensity Differential Scattering....Pages 184-193
An Instrument for Virus Identification by Polarized Light Scattering: A Preliminary Report....Pages 194-197
A New Method for the Rapid Identification and Differentiation of Pathogenic Microorganisms Using Fourier Transform Infrared Spectroscopy....Pages 198-206
The Use of Microcalorimetry and Impedance in Clinical Microbiology....Pages 207-214
The Photometric Analysis of Microbial Growth for the Identification and Antimicrobial Susceptibility Testing of Bacterial Pathogens....Pages 215-221
The Application of Impediometry to Rapid Antibiotic Susceptibility Testing and Early Detection of Bacterial Growth....Pages 222-230
Chromatographic Analysis for Identification of Microorganisms and Diagnosis of Infection: An Introduction....Pages 231-231
Chromatographic Analysis of Bacterial Cellular Components....Pages 232-238
Analysis of Bacterial Metabolites and Components by Computerized GC/MS System — From Shorter Chain Acids to Very Long-Chain Compounds up to C 80 ....Pages 239-247
Gas Chromatographic Analysis of Clinical Samples for Rapid Diagnosis of Infection....Pages 248-254
Pyrolysis Gas-Liquid Chromatography for Identification of Microorganisms....Pages 255-264
Taxonomic Calculations Based on Fatty Acid Spectra of Bacteria. Requirements for Chromatographic Analysis of Fatty Acids....Pages 265-273
Extraction and Chromatographic Analysis of Characteristic Mycobacterial Lipids....Pages 274-282
Progress in Methods for More Rapid Isolation of Agents Responsible for Bacteremia....Pages 283-290
Mechanised Plate Spreading — From Concept to Realisation....Pages 291-296
COBAS Bact: The Centrifugal Approach to Clinical Bacteriology....Pages 297-304
Short-Term Methods for Isolation and Enumeration of Bacteria in the Respiratory Tract....Pages 305-308
Some New Media for Isolation and Rapid Identification of Bacteria....Pages 309-316
Current Trends and Practical Application in Rapid Viral Diagnosis....Pages 317-328
Respiratory Viral Antigen Detection by Solid-Phase Immunoassays: Comparison with Other Diagnostic Tests and Application for Routine Diagnosis....Pages 329-337
Coagglutination Procedures and Affinity Chromatography in Antigen and Antibody Detection....Pages 338-351
Instrumental Methods for Antigen Detection....Pages 352-356
Detection of Group A Streptococcal Antigen by Coagglutination and Latex Agglutination....Pages 357-366
Growth-Independent Rapid Automated Enzyme Identification System....Pages 367-376
An Automatic Micromethod for the Identification of Gram-Negative Bacilli by Carbon Substrate Assimilation Tests....Pages 377-389
Rapid Typing of Pili of Pathogenic Escherichia coli by Dispersive Immunoelectron Microscopy....Pages 390-400
Enzyme Immunoassays Using Fluorescent Substrates....Pages 401-407
Identification of Bacteria by Flow Immunofluorescence....Pages 408-413
The Principle of Time-Resolved Fluorometry....Pages 414-421
Microbiologic Applications of Fluoroimmunoassay....Pages 422-428
Detection of Viral Antigens by Time-Resolved Fluoroimmunoassay....Pages 429-437
Monitoring Microbial Growth by Bioluminescent ATP Assay....Pages 438-447
Bioluminescent Assay of Bacterial ATP as a Tool in Clinical Microbiology....Pages 448-454
Bacteriuria Detection in an Outpatient Setting: Comparison of Several Methods Including an Improved Assay of Bacterial ATP....Pages 455-460
Analytical Use of Luminous Bacteria in Veterinary and Medical Microbiology....Pages 461-466
Antimicrobial Susceptibility Testing Using Fluorogenic Enzyme Substrates....Pages 467-474
Antimicrobial Susceptibility Tests: Automation and Mechanization....Pages 475-483
Techniques for In Vitro Tests on New Antibiotics....Pages 484-489
MIC Reading with the Biology Laboratory Computer....Pages 490-496
The Alteration of Microbial Growth Curves by Antibiotics....Pages 497-503
Clinical Needs for Determination of Antimicrobial Agents in Fluids and Tissues....Pages 504-509
Methods for Detection of Antimicrobial-Induced Changes in the Normal Human Oropharyngeal and Colon Microflora....Pages 510-514
Chromatographic Methods for Determination of Antimicrobial Agents in Fluids and Tissues....Pages 515-522
Microbiological and Immunological Assays of Antimicrobials....Pages 523-528
Computerized Pharmacokinetic Evaluation of Antibiotics in Body Fluids and Tissues: An Overview....Pages 529-537
Data Handling and Retrieval in Clinical Virology by Small Decentralized Computers....Pages 538-556
Application of Small Decentralized Computers in the Management of Bacteriologic Data in the Clinical Laboratory....Pages 557-563
MicroLab and MicroSeek — A New Approach to Computing in Medical Microbiology....Pages 564-569
The Use of the Computer to Aid Decision Making by the Food Microbiologist....Pages 570-579
Computer-Assisted Reading System for Microbiological Tests....Pages 580-588
Problems in Operation and Data Transfer in a Multicomputer Environment....Pages 589-592
A Philosophical Approach to Rapid Methods for Industrial Food Control....Pages 593-602
Essentials of Enumeration and Identification Methods for Microorganisms Which Interfere with the Safety of Foods with Particular Reference to Simplification Avoiding the Use of Nonconventional Instrumentation....Pages 603-621
Membrane Filter Techniques for Food Microbiological Analysis....Pages 622-628
Status and Future of the Direct Epifluorescent Filter Technique....Pages 629-637
Application of the Limulus Test for Detection of Gram-Negative Microorganisms in Milk and Dairy Products....Pages 638-648
Enzyme-Linked Immunosorbent Assay of Staphylococcal Enterotoxins in Foods....Pages 649-655
Procedures and Methods for One-Day Analysis of Microbial Loads in Foods....Pages 656-664
The Potential of Chromatographic Techniques for the Manipulation of Viable Micro-Organisms....Pages 665-678
Electrical Impedance Method for Determining Microbial Quality of Foods....Pages 679-687
Are Rapid and Sensitive Microbiological Procedures Necessary for Monitoring the Environment?....Pages 688-695
Searching for Rapid Methods in Environmental Bacteriology....Pages 696-707
Application of Automated and Rapid Microbiologic Assays in Various Health-Care Environments....Pages 708-715
Problems in Detecting Water-Borne Viruses by Rapid Methods....Pages 716-726
Detection and Identification of Viruses in Water by Immunoadsorbance and Energy Transfer....Pages 727-730