Rapid Cycle Real-Time PCR — Methods and Applications: Microbiology and Food Analysis

Front Matter....Pages I-IX
Introduction for Microbiology Volume of Rapid Cycle Real-Time PCR....Pages 1-2
Applications and Challenges of Real-Time PCR for the Clinical Microbiology Laboratory....Pages 3-27
Front Matter....Pages 29-30
Rapid Detection and Simultaneous Differentiation of Bordetella pertussis and Bordetella parapertussis in Clinical Specimens by LightCycler PCR....Pages 31-43
Rapid Detection and Simultaneous Differentiation of Legionella spp. and L. pneumophila in Potable Water Samples and Respiratory Specimens by LightCycler PCR....Pages 45-57
Homogeneous Assays for the Rapid PCR detection of Burkholderia pseudomallei 16S rRNA gene on a Real-Time Fluorometric Capillary Thermocycler....Pages 59-69
Rapid Detection of Toxigenic Corynebacterium diphtheriae by LightCycler PCR....Pages 71-81
Rapid Detection of Resistance Associated Mutations in Mycobacterium tuberculosis by LightCycler PCR....Pages 83-91
Duplex LightCycler PCR Assay for the Rapid Detection of Methicillin-Resistant Staphylococcus aureus and Simultaneous Species Confirmation....Pages 93-105
Rapid Detection of Group B Streptococci Using the LightCycler Instrument....Pages 107-114
Rapid Detection and Quantification of Chlamydia trachomatis in Clinical Specimens by LightCycler PCR....Pages 115-132
Quantification of Toxoplasma gondii in Amniotic Fluid by Rapid Cycle Real-Time PCR....Pages 133-138
Genospecies-Specific Melting Temperature of the recA PCR Product for the Detection of Borrelia burgdorferi sensu lato and Differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi sensu stricto....Pages 139-147
Rapid and Specific Detection of Coxiella burnetii by LightCycler PCR....Pages 149-154
Molecular Identification of Campylobacter coli and Campylobacter jejuni Using Automated DNA Extraction and Real-Time PCR with Species-Specific TaqMan Probes....Pages 155-159
Rapid and Specific Detection of Plesiomonas shigelloides Directly from Stool by LightCycler PCR....Pages 161-169
Quantification of Thermostable Direct Hemolysin-Producing Vibrio Parahaemolyticus from Foods Assumed to Cause Food Poisoning Using the LightCycler Instrument....Pages 171-176
Front Matter....Pages 177-177
Quantification and Speciation of Fungal DNA in Clinical Specimens Using the LightCycler Instrument....Pages 179-186
Front Matter....Pages 187-187
Development, Implementation, and Optimization of LightCycler PCR Assays for Detection of Herpes Simplex Virus and Varicella-Zoster Virus from Clinical Specimens....Pages 189-200
Qualitative Detection of Herpes Simplex Virus DNA in the Routine Diagnostic Laboratory....Pages 201-209
Use of Real-Time PCR to Monitor Human Herpesvirus 6 (HHV-6) Reactivation....Pages 211-216
Front Matter....Pages 187-187
Simultaneous Identification of Five HCV Genotypes in a Single Reaction....Pages 217-225
Rapid Detection of West Nile Virus....Pages 227-234
Use of Rapid Cycle Real-Time PCR for the Detection of Rabies Virus....Pages 235-240
Development of One-Step RT-PCR for the Detection of an RNA Virus, Dengue, on the Capillary Real-Time Thermocycler....Pages 241-248
Front Matter....Pages 249-249
Variation Analysis of Seven LightCycler Based Real-Time PCR Systems to Detect Genetically Modified Products (RRS, Bt176, Bt11, Mon810, T25, Lectin, Invertase)....Pages 251-258