Quorum Sensing: Methods and Protocols

Front Matter ....Pages i-xvi
Front Matter ....Pages 1-1
 Use of Whole-Cell Bioassays for Screening Quorum Signaling, Quorum Interference, and Biofilm Dispersion  (Starla G. Thornhill, Robert J. C. McLean)....Pages 3-24
 Detection of 2-Alkyl-4-Quinolones Using Biosensors  (Matthew P. Fletcher, Stephen P. Diggle, Miguel Cámara, Paul Williams)....Pages 25-34
 “Hot Stuff”: The Many Uses of a Radiolabel Assay in Detecting Acyl-Homoserine Lactone Quorum-Sensing Signals  (Amy L. Schaefer, Caroline S. Harwood, E. Peter Greenberg)....Pages 35-47
 Liquid Chromatography/Mass Spectrometry (LC/MS) for the Detection and Quantification of N-Acyl-L-Homoserine Lactones (AHLs) and 4-Hydroxy-2-Alkylquinolines (HAQs)  (François Lépine, Sylvain Milot, Marie-Christine Groleau, Eric Déziel)....Pages 49-59
 Detection of the Bacterial Quorum-Sensing Signaling Molecules N-Acyl-Homoserine Lactones (HSL) and N-Acyl-Homoserine (HS) with an Enzyme-Linked Immunosorbent Assay (ELISA) and via Ultrahigh-Performance Liquid Chromatography Coupled to Mass Spectrometry (UHPLC-MS)  (Michael Rothballer, Jenny Uhl, Josie Kunze, Philippe Schmitt-Kopplin, Anton Hartmann)....Pages 61-72
 Biosensors for the Detection and Quantification of AI-2 Class Quorum-Sensing Compounds  (Sathish Rajamani, Richard Sayre)....Pages 73-88
 Detection of Agr-Type Autoinducing Peptides Produced by Staphylococcus aureus  (Ewan J. Murray, Paul Williams)....Pages 89-96
 Ultra-Performance Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of Diffusible Signal Factor (DSF) Family Quorum-Sensing Signals  (Lian Zhou, Shuang Sun, Wei Zhang, Ya-Wen He)....Pages 97-105
 Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode  (Alyah Buzid, John H. T. Luong, F. Jerry Reen, Fergal O’Gara, Jeremy D. Glennon, Gerard P. McGlacken)....Pages 107-116
 Detection and Quantification of Butyrolactones from Streptomyces  (Marc Biarnes-Carrera, Rainer Breitling, Eriko Takano)....Pages 117-128
Front Matter ....Pages 129-129
 Fluorescence Quenching Studies of γ-Butyrolactone-Binding Protein (CprB) from Streptomyces coelicolor A3(2)  (Jessy Mariam, Ruchi Anand)....Pages 131-143
 Methods to Study Solo/Orphan Quorum-Sensing Receptors  (Vittorio Venturi, Sujatha Subramoni, Anice Sabag-Daigle, Brian M. M. Ahmer)....Pages 145-159
 Enzymatic Assays to Investigate Acyl-Homoserine Lactone Autoinducer Synthases  (Daniel Shin, Rajesh Nagarajan)....Pages 161-176
 Global Expression Analysis of Quorum Sensing-Controlled Genes by RNAseq  (Charlotte D. Majerczyk)....Pages 177-192
 Identification of AHL- and BDSF-Controlled Proteins in Burkholderia cenocepacia by Proteomics  (Yilei Liu, Gabriella Pessi, Katharina Riedel, Leo Eberl)....Pages 193-202
 Imaging N-Acyl Homoserine Lactone Quorum Sensing In Vivo  (Louise Dahl Hultqvist, Maria Alhede, Tim Holm Jakobsen, Michael Givskov, Thomas Bjarnsholt)....Pages 203-212
 Assessing Pseudomonas aeruginosa Autoinducer Effects on Mammalian Epithelial Cells  (Jake Everett, Rebecca Gabrilska, Kendra P. Rumbaugh, Elena Vikström)....Pages 213-225
 Animal Models for Pseudomonas aeruginosa Quorum Sensing Studies  (Damien Maura, Arunava Bandyopadhaya, Laurence G. Rahme)....Pages 227-241
 Methods to Study Quorum Sensing-Dependent Virulence and Movement of Phytopathogens In Planta  (Shulamit Manulis-Sasson, Laura Chalupowicz)....Pages 243-252
 Differential Equations Models to Study Quorum Sensing  (Judith Pérez-Velázquez, Burkhard A. Hense)....Pages 253-271
Front Matter ....Pages 273-273
 Qualitative and Quantitative Determination of Quorum Sensing Inhibition In Vitro  (Tim Holm Jakobsen, Maria Alhede, Louise Dahl Hultqvist, Thomas Bjarnsholt, Michael Givskov)....Pages 275-285
 A Coculture-Based Approach for Screening Campaigns Aimed at Identifying Novel Pseudomonas aeruginosa Quorum Sensing Inhibitors  (Giordano Rampioni, Giulia Giallonardi, Francesca D’Angelo, Livia Leoni)....Pages 287-296
 A Culture-Dependent Method for the Identification of Quorum Quenching Enzymes of Microbial Origin  (Kaihao Tang, Xiao-Hua Zhang)....Pages 297-309
 Directed Evolution of Quorum-Quenching Enzymes: A Method for the Construction of a Directed Evolution Platform and Characterization of a Quorum-Quenching Lactonase from Geobacillus kaustophilus  (Maybelle Kho Go, Jeng Yeong Chow, Wen Shan Yew)....Pages 311-323
 Generation of High-Sensitivity Monoclonal Antibodies Specific for Homoserine Lactones  (Soumya Palliyil)....Pages 325-352
 Identification of AI-2 Quorum Sensing Inhibitors in Vibrio harveyi Through Structure-Based Virtual Screening  (Tianyu Jiang, Peng Zhu, Lupei Du, Minyong Li)....Pages 353-362
 Identification of Staphylococcal Quorum Sensing Inhibitors by Quantification of õ-Hemolysin with High Performance Liquid Chromatography  (Cassandra L. Quave, Alexander R. Horswill)....Pages 363-370
Back Matter ....Pages 371-375