Purine Metabolism in Man-IV: Part B: Biochemical, Immunological, and Cancer Research

Front Matter....Pages i-xvi
Evidence of a New Type Of Phosphoribosylpyrophosphate Synthetase With Abnormal Kinetic Properties in Hyperuricemias....Pages 1-4
Radioimmunoassay Studies of Human Phosphoribosylpyrophosphate Synthetase....Pages 5-10
Synthesis of ( 14 C)-Ribose-5-Phosphate and ( 14 C)-Phosphoribosyl-Pyrophosphate and Their use in New Enzyme Assays....Pages 11-13
Purine Synthesis During Amino Acid Starvation of Lymphoblasts With HPRT Deficiency or PP-Ribose-P Synthetase Overactivity Decreases Less Than in Normal Cells....Pages 15-19
Purine Synthesis and Salvage in Brain and Liver....Pages 21-26
Kinetic Studies of Hypoxanthine-Guanine Phosphoribosyltransferase in Intact Cells....Pages 27-31
Hypoxanthine-Guanine Phosphoribosyltransferase in Human Lymphoblastoid Cells: Confirmation of Four Structural Variants and Demonstration of a New Variant (HPRT Ann Arbor )....Pages 33-38
The Primary Structure and Posttranslational Modification of Human Hypoxanthine-Guanine Phosphoribosyltransferase....Pages 39-44
Studies of the Catalytically-Active Form of Hypoxanthine-Guanine Phosphoribosyltransferase from Yeast....Pages 45-50
Human Lymphocytic Ecto-5′-NT: Its Determination and Partial Characterization....Pages 51-54
Distribution of Ecto-5′-Nucleotidase in Plasmamembrane Subfractions of Human Lymphoblastoid Cells....Pages 55-58
AMP Deaminase Isozymes in Human Blood Cells....Pages 59-62
Immunohistochemical Localization of Adenosine Deaminase in Rat and Calf Tissues....Pages 63-66
Characterization of Adenosine Deaminating Activity in Normal and Adenosine Deaminase Deficient Human Tissue....Pages 67-70
S-Adenosylhomocysteine Hydrolase Activity in Defects of Enzymes of Purine Metabolism and Inhibition by Various Purine Compounds....Pages 71-74
Deoxyguanosine Kinase from Human Placenta....Pages 75-78
Phosphorylation of Deoxyguanosine in Rat Liver Mitochondria....Pages 79-82
5′-Methylthioadenosine is the Major Source of Adenine in Human Cells....Pages 83-88
Determination of a Histidine Residue at the Yeast Orotate Phosphoribosyltransferase Active-Site....Pages 89-92
Changes in Purine Salvage Pathway Enzyme Activities During Human Lymphocyte Differentiation Induced by Thymosin Fraction 5....Pages 93-98
Purine Metabolism in Human Thymocyte Subsets: Relevance for Lymphocytic Differentiation....Pages 99-106
Purine Metabolism in Splenocytes and Thymocytes of Various Mammalian Species....Pages 107-110
Purine Interconversion Pathways in T, B, Tγ and T-Tγ Cells from Human Peripheral Blood....Pages 111-118
Metabolism of Guanosine and Deoxyguanosine in Mammalian Lymphocytes and their Influence on Mitogenic Stimulation....Pages 119-124
Concentration of Nucleotides in Peripheral Blood Lymphocytes of Various Mammalian Species....Pages 125-128
Plasma Membrane 5′-Nucleotidase and other Purine Enzymes in Murine Lymphocytes....Pages 129-132
Biochemical Basis for Lymphocyte Dysfunction in Adenosine Deaminase and Purine Nucleoside Phosphorylase Deficiencies....Pages 133-139
Absence of Lymphocyte Ecto-5′-Nucleotidase in Infants with Reticuloendotheliosis and Immunodeficiency....Pages 141-146
Regulation of Human Lymphoblast Ecto-5′-Nucleotidase by Zinc....Pages 147-152
Decreased T Cell Ecto-5′-Nucleotidase Activity in Agammaglobulinemia Patients is Not Due to Altered Proportions of Helper and Suppressor T Cells as Defined by the Monoclonal Antibodies Okt4 and Okt8....Pages 153-158
Metabolic Findings in a Patient with Adenosine Deaminase Deficiency and Severe Combined Immunodeficiency....Pages 159-162
Purine Metabolism in Intact Cells from a Purine Nucleoside Phosphorylase Deficient Child....Pages 163-166
Inosine Formation from Hypoxanthine by Intact Erythrocytes and Fibroblasts of an Immunodeficient Child with Purine Nucleoside Phosphorylase Deficiency....Pages 167-170
Mononuclear Cells in S-Phase in a Patient with Purine Nucleoside Phosphorylase Deficiency....Pages 171-174
Involvement of Dietary Nucleotides in T Lymphocyte Function....Pages 175-178
Nucleotide Levels in Peripheral Blood Mononuclear Cells of Immunodeficient Children: Problems of Measurement....Pages 179-182
Inhibition of Murine Suppressor T Cell Development by 2′-Deoxyguanosine in Vivo ....Pages 183-188
Biochemical and Immunological Evaluation of Long Term Coformycin Administration in the Mouse....Pages 189-192
Increased Incidence of Homogeneous Immunoglobulins in Irradiated, Reconstituted Mice After Prolonged Treatment with 2′-Deoxyguanosine....Pages 193-197
S-Adenosylhomocysteine Hydrolase as a Pharmacological Target for the Inhibition of Transmethylation....Pages 199-203
Selective Inhibition of Various Mitogen Responses in Human Lymphocytes....Pages 205-210
Sensitivity of Human T and B Lymphoblasts to Immunoregulatory Drugs....Pages 211-214
Inhibition of in Vitro Lymphocyte Proliferation and B Cell Differentiation by Deoxyguanosine: Evidence for Separate Mechanisms....Pages 215-220
Effect of Purine Nucleosides on Rat Macrophage Function....Pages 221-226
Purine Metabolism in Rat Macrophages....Pages 227-232
Purine Metabolizing Enzyme Activities in Lymphoblastic Leukemia....Pages 233-240
Purine and Pyrimidine Enzyme Markers in Human Lymphoid Malignancies....Pages 241-247
Ecto-5′-Nucleotidase as a Leukemia Marker....Pages 249-253
Immunohistochemical Localization of Adenosine Deaminase in Human Lymphoid Tissues and Lymphomas....Pages 255-259
5′Nucleotidase and Adenosine Deaminase Activities in Human Lymphocytes and Lymphoblastoid Cell Lines....Pages 261-266
Properties and Activities of Phosphoribomutase in Human Leukemic Cells....Pages 267-272
Uptake of AMP into K 562 and HL-60 Leukemic Cell Lines....Pages 273-278
5′-Methylthioadenosine Phosphorylase Deficiency in Malignant Cells: Recessive Expression of the Defective Phenotype in Intra-Species (Mouse X Mouse) Hybrids....Pages 279-283
The Activity of Adenosine Desaminase in Serum and Leukocytes of Leukemic Patients....Pages 285-288
Uptake of Thymidine by Plasma Membrane Vesicles of L5178Y Murine Leukemic Cells....Pages 289-292
Adenosine Deaminase Isozymes in Transformed Cells....Pages 293-298
Adenosine Deaminase (ADA; E.C. no. 3.5.4.4.) in Colorectal Adenocarcinoma in Man....Pages 299-303
Biochemical Basis for the Differential Effects of Deoxycoformycin on Human Leukemias....Pages 305-308
Sensitivity of T-Leukemic Cells to Deoxyguanosine and Arabinosyl Guanine....Pages 309-314
Exploiting Biochemical Differences in Leukemia....Pages 315-320
Effects of 3-Deazaguanosine and 3-Deazaguanine on the Growth and Maturation of the Human Promyelocytic Leukemia Cell Line, HL-60....Pages 321-325
Intralymphocytic Adenosine Deaminase (ADA) and Purine Nucleoside Phosphorylase (PNPase) Activity in Chronic Lymphocytic Leukemia (CLL) : Effect of Thymostimulin....Pages 327-332
Deoxyadenosine Induced G 1 Phase Arrest in Leukemic T Cells....Pages 333-338
Poly(A) + RNA as a Possible Target for Deoxyadenosine Induced G 1 /G 0 Lymphotoxicity....Pages 339-344
Modulation of Arabinosyladenine Metabolism by 2′-Deoxycoformycin in the Therapy of Human Acute Leukemia....Pages 345-350
Cell Cycle Independent Lymphocytotoxicity of 2-Chlorodeoxyadenosine....Pages 351-356
Ecto-Adenosine Triphosphatase: An Enzyme Correlating with Resistance to Deoxynucleoside Growth Inhibition....Pages 357-362
Purine Deoxyribonucleoside Induced Hepatotoxicity in the Mouse....Pages 363-366
6-Mercaptopurine: Pharmacokinetics in Animals and Preliminary Results in Children....Pages 367-370
Enzyme Inhibition by Phosphonate Analogues of dTTP....Pages 371-374
Inhibition of Thymidylate Synthetase by the New Quinazoline Antifolate, CB 3717; Enzyme Purification and Kinetics....Pages 375-378
Plasma Nucleoside and Base Levels Following Treatment with the New Thymidylate Synthetase Inhibitor CB 3717....Pages 379-382
Influence of Cytostatics on ATP-Levels of Leukemic Cells....Pages 383-388
Thymidine Sensitivity and Deoxynucleotide Pools of Human Lymphoid and Melanoma Cells in Vitro....Pages 389-393
High Uridine Catabolic Activity in Cultured Human Melanoma Cells....Pages 395-399
Synergism Between Purines and Thymidine (TDR) in Reversal of Methotrexate (MTX) Toxicity in Mice Using Continuous Infusions....Pages 401-405
Cell-Cycle Dependent Variation in the Levels of Deoxyribonucleoside Triphosphate in Mouse T-Lymphoma Cells....Pages 407-410
Tumor Specific Conversion of a Pyrimidine Antimetabolite....Pages 411-414
Isotachophoretic Analysis of Some Compounds Involved in Energy Metabolism in Normal and Pathological Human Muscle Extracts....Pages 415-418
Adenine Metabolism in Rat Skeletal Muscle....Pages 419-424
Purine Nucleotide Metabolism in Primary Rat Skeletal Muscle Cultures....Pages 425-429
Metabolism of AMP in Muscle Extracts from Patients with Deficient Activity of Myoadenylate Deaminase....Pages 431-436
Effect of Hypoxia and Exercise on Nucleotide Metabolism in Man....Pages 437-442
Malignant Hyperthermia: Adenine Incorporation and Adenine Metabolism in Human Platelets, Influenced by Halothane....Pages 443-446
Enzymes of Purine Metabolism in Muscle Specimens from Patients with Duchenne-Type Muscular Dystrophy....Pages 447-450
X-Linked Recessive (Duchenne) Muscular Dystrophy (DMD) and Purine Metabolism: Effects of Oral Allopurinol and Adenylate....Pages 451-456
Non-Specific Adenylate Deaminase from Snail Foot Muscle....Pages 457-464
Potential Role of Non-Specific Snail Muscle Adenylate Deaminase in Operating of NADH-Dependent Dehydrogenases....Pages 465-468
Adenine Nucleotide Biosynthesis in Cardiac Muscle: Regulation and Intervention....Pages 469-475
Ribose Prevents the Propranolol-Induced Reduction of Myocardial Adenine Nucleotide Biosynthesis....Pages 477-481
Balance of Purine Nucleotides and Catabolites in the Isolated Ischemic Rat Heart....Pages 483-486
Inosine Incorporation into Adenine- and Guanine Nucleotides in the Isolated Perfused Rat Heart is Stimulated After Ischemia....Pages 487-490
The Ca-Antagonist Nifedipine Reduces Purine Nucleoside and Oxypurine Release from Ischemic Heart....Pages 491-496
Combined Use of Radioenzymatic Assay and High Pressure Liquid Chromatography for the Detection of Myocardial Xanthine Oxidase/Dehydrogenase....Pages 497-500
Purification and Characterization of Pig Heart AMP-Deaminase....Pages 501-504
AMP Deaminase from Necrotic Rat Heart....Pages 505-507
Back Matter....Pages 509-520